LOCUS       sequence-378114-       14172 bp    DNA     circular SYN 15-APR-2025
DEFINITION  To act as a 2-input state of the NOR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14172)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 14172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
FEATURES             Location/Qualifiers
     source          1..14172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          978..1166
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     protein_bind    2193..2226
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2261..2435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2823..4475
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2889..2907)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4495..4695
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5038..5093
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5098..5118
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          5896..6084
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7046..7220
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    7562..7617
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7625..7645
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          8329..8517
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    9141..9315
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    9385..9406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9421..9426,9427..9444,9445..9451)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9459..9475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9464..9486
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9483..9499
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9483..9499
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9495..9818
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    9512..9568
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9569..9586)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9569..9585)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9578..9600)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9794..9813)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    9950..9974
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      10062..10772
                     /label=oriV
                     /note="incP origin of replication"
     promoter        10793..10897
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10898..11692
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11863..12451
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12352..12371
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12787..13935
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    14081..14105
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac ccaacaaaca acattacaat tactatttac aattacaatg acttcaaagg
      961 tgtatgatcc tgagcaggta agtttctgct tctacctttg atatatatat aataattatc
     1021 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     1081 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     1141 tatgaccaaa atttgttgat gtgcagagaa aaagaatgat aactggtcca caatggtggg
     1201 caagatgtaa acagatgaac gtgcttgatt ctttcattaa ttactacgat tcagaaaagc
     1261 acgctgagaa tgcagttatc tttcttcacg gaaacgctgc atcttcatat ttgtggagac
     1321 atgttgtgcc tcacattgaa ccagtggcta ggtgtattat cccagatctt atcggaatgg
     1381 gaaagtctgg taaatctgga aatggttctt acagactttt ggatcattat aagtacctca
     1441 cagcttggtt cgaacttctc aacttaccta agaagattat tttcgttgga cacgattggg
     1501 gtgcttgctt ggcattccat tactcatacg aacaccaaga taagatcaaa gctatagttc
     1561 atgcagagag tgttgtggat gtgatagaat cttgggatga gtggccagat attgaagagg
     1621 atattgcttt gatcaagtct gaagagggag aaaagatggt tctcgaaaat aactttttcg
     1681 tggaaactat gcttccttca aagattatga gaaaattgga accagaagag tttgctgcat
     1741 atctcgaacc tttcaaggaa aaaggagagg ttagaaggcc aacactcagt tggcctaggg
     1801 aaataccatt agttaaggga ggtaaacctg atgttgtgca gatcgtgaga aattataacg
     1861 cttaccttag ggcatcagat gatttgccta agatgttcat cgagtcagat ccaggatttt
     1921 tcagtaatgc tatagttgaa ggtgcaaaga aatttccaaa caccgagttc gttaaggtga
     1981 aaggattgca tttctcacaa gaagatgctc ctgatgagat gggaaagtat attaagtctt
     2041 ttgttgaaag agtgctcaaa aacgagcagg gttctcgatc tgatgcttct gaagtaacgt
     2101 caacaagctc ccagtctgag gtatgcacag tggaaacgcc aggttgtgtt ggttcttaag
     2161 gttgcttaag aataagtaat tcttaagcaa ccgaagttcc tattctctag aaagtatagg
     2221 aacttcgctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     2281 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     2341 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2401 tcctaaaacc aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc
     2461 aacactcgaa tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta
     2521 tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc
     2581 caaaattgta atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga
     2641 ctataaatac caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc
     2701 gcaaccttcg attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac
     2761 ctactacaaa caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag
     2821 taatggagga cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg
     2881 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2941 ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt
     3001 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     3061 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     3121 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca
     3181 tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     3241 aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg
     3301 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     3361 attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg
     3421 gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct
     3481 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     3541 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     3601 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt
     3661 acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc
     3721 tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt
     3781 cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat
     3841 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3901 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3961 aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg
     4021 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     4081 ctggagacat agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt
     4141 ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac
     4201 aacaccccaa catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac
     4261 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     4321 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     4381 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     4441 taaaggccaa gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc
     4501 tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg
     4561 taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt
     4621 tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta
     4681 tatttctttc aagatcgcta ctagaattcg agctcggagc caacactcga atccccaccc
     4741 gttggaccaa acccggctca ttaagcgtcg gttcagattt atttccttta tttaaaaaaa
     4801 aggaaagggt aaaaaataga aaattggaaa cagttaaagc ccaaaattgt aatttaccga
     4861 gaattgtaaa tttacctgaa aaccctacgc tatagtttcg actataaata ccaaacttag
     4921 gacctcactt cagaatcccc tcgtcgctgc gtctctctcc cgcaaccttc gattttcgtt
     4981 tattcgcatc catcggagag agaaaacaat caataagcga cctactgtat ttttacaaca
     5041 attaccaaca acaacaaaca acaaacaaca ttacaattac tatttacaat tacaatgccc
     5101 aagaaaaagc ggaaggtgtc cagctacatg gacctggtgg acgacgagcc cgccaccctg
     5161 taccacaagt tcgtggaatg cctgaaggcc ggcgagaact tctgcggcga taagctgagc
     5221 ggcatcatca ccatggccat tctgaaggcc atcaaggccc tgaccgaagt gaagaaaacc
     5281 accttcaaca agtacaagac caccatcaag cagggcctgc agtacgacgt gggcagcagc
     5341 accatcagct tcgtgtacca cctgaaggac tgcgacgagc tgagcagagg cctgagcgac
     5401 gccttcgagc cctacaagtt caagatcaag agcaacaaag aggccaccag cttcaagacc
     5461 ctgttcaggg gccctagctt cggcagccag aagaactggc ggaagaaaga ggtggaccgc
     5521 gaggtggaca acctgttcca cagcaccgag acagacgaga gcatcttcaa gttcatcctg
     5581 aacaccctgg acagcatcga aacccagacc aacaccgacc ggcagaaaac cgtgctgacc
     5641 tttatcctgc tgatgacctt cttcaactgc tgccggaaca acgacctgat gaacgtggac
     5701 cccagcacct tcaagatcgt gaagaacaag tttgtgggct acctgctgca ggctgaagtg
     5761 aagcagacca agaccagaaa gagccggaat atcttcttct tccccatccg ggaaaaccgc
     5821 ttcgacctgt tcctggccct gcacgacttc ttcagaacct gccagcccac ccccaagagc
     5881 agactgagcg atcaggtaag tttctgcttc tacctttgat atatatataa taattatcat
     5941 taattagtag taatataata tttcaaatat ttttttcaaa ataaaagaat gtagtatata
     6001 gcaattgctt ttctgtagtt tataagtgtg tatattttaa tttataactt ttctaatata
     6061 tgaccaaaat ttgttgatgt gcaggtgtcc gagcagaagt ggcagctgtt ccgggacagc
     6121 atggtcatcg actacaaccg gttctttcgg aagttccccg ccagccccat cttcgccatt
     6181 aagcacggcc ccaagtccca cctgggccgg catctgatga acagctttct gcacaagaac
     6241 gagctggaca gctgggccaa cagcctgggc aattggagca gctcccagaa ccagagagag
     6301 agcggcgcca gactgggcta cacacacggc ggaagagatc tgccccagcc cctgtttggc
     6361 ttcctggccg gatactgcgt gcggaacgaa gagggccaca tcgtgggcct gggcctggaa
     6421 aaggacatca acgatctgtt cgacggcatc atggaccccc tgaacgagaa agaggacacc
     6481 gagatctgcg agagctacgg cgagtgggcc aagattgtgt ccaaggacgt gctgatcttc
     6541 ctgaagagat accacagcaa gaacgcctgt cggagatacc agaacagcac cctgtatgcc
     6601 cggaccttcc tgaaaaccga gagcgtgacc ctgagcggct ccaagggcag cgaggaacct
     6661 tctagccctg tgcggatccc catcctgagc atgggaaagg ccagcccctc cgagggaaga
     6721 aagctgagag ccagcgagca cgccaacgac gacaacgaga tcgagaagat cgacagcgac
     6781 agcagccaga gcgaagagat ccctatcgag atgagcgact ccgaggacga gacaaccgcc
     6841 agcaacatca gcggcatcta cctggacatg agcaaggcca actccaacgt ggtgtacagc
     6901 ccccctagcc agacaggcag agctgctggc gccggaagaa aaagaggcgt gggaggcaga
     6961 cggaccgtgg aaagcaagcg gagaagagtg ctggccccca tcaaccggtg agcttctcta
     7021 gctagagtcg atcgacaagc tcgagtttct ccataataat gtgtgagtag ttcccagata
     7081 agggaattag ggttcctata gggtttcgct catgtgttga gcatataaga aacccttagt
     7141 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     7201 ccagtactaa aatccagatc gctttacgaa ttcccatggg gagccaacac tcgaatcccc
     7261 acccgttgga ccaaacccgg ctcattaagc gtcggttcag atttatttcc tttatttaaa
     7321 aaaaaggaaa gggtaaaaaa tagaaaattg gaaacagtta aagcccaaaa ttgtaattta
     7381 ccgagaattg taaatttacc tgaaaaccct acgctatagt ttcgactata aataccaaac
     7441 ttaggacctc acttcagaat cccctcgtcg ctgcgtctct ctcccgcaac cttcgatttt
     7501 cgtttattcg catccatcgg agagagaaaa caatcaataa gcgacctact gtatttttac
     7561 aacaattacc aacaacaaca aacaacaaac aacattacaa ttactattta caattacaat
     7621 ggctcctaag aagaagagga aggtgatgag ccagttcgac atcctgtgca agaccccccc
     7681 caaggtgctg gtgcggcagt tcgtggagag attcgagagg cccagcggcg agaagatcgc
     7741 cagctgtgcc gccgagctga cctacctgtg ctggatgatc acccacaacg gcaccgccat
     7801 caagagggcc accttcatga gctacaacac catcatcagc aacagcctga gcttcgacat
     7861 cgtgaacaag agcctgcagt tcaagtacaa gacccagaag gccaccatcc tggaggccag
     7921 cctgaagaag ctgatccccg cctgggagtt caccatcatc ccttacaacg gccagaagca
     7981 ccagagcgac atcaccgaca tcgtgtccag cctgcagctg cagttcgaga gcagcgagga
     8041 ggccgacaag ggcaacagcc acagcaagaa gatgctgaag gccctgctgt ccgagggcga
     8101 gagcatctgg gagatcaccg agaagatcct gaacagcttc gagtacacca gcaggttcac
     8161 caagaccaag accctgtacc agttcctgtt cctggccaca ttcatcaact gcggcaggtt
     8221 cagcgacatc aagaacgtgg accccaagag cttcaagctg gtgcagaaca agtacctggg
     8281 cgtgatcatt cagtgcctgg tgaccgagac taagacaagc gtgtccaggt aagtttctgc
     8341 ttctaccttt gatatatata taataattat cattaattag tagtaatata atatttcaaa
     8401 tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta gtttataagt
     8461 gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga tgtgcaggca
     8521 catctacttt ttcagcgcca gaggcaggat cgaccccctg gtgtacctgg acgagttcct
     8581 gaggaacagc gagcccgtgc tgaagagagt gaacaggacc ggcaacagca gcagcaacaa
     8641 gcaggagtac cagctgctga aggacaacct ggtgcgcagc tacaacaagg ccctgaagaa
     8701 gaacgccccc taccccatct tcgctatcaa gaacggccct aagagccaca tcggcaggca
     8761 cctgatgacc agctttctga gcatgaaggg cctgaccgag ctgacaaacg tggtgggcaa
     8821 ctggagcgac aagagggcct ccgccgtggc caggaccacc tacacccacc agatcaccgc
     8881 catccccgac cactacttcg ccctggtgtc caggtactac gcctacgacc ccatcagcaa
     8941 ggagatgatc gccctgaagg acgagactaa ccccatcgag gagtggcagc acatcgagca
     9001 gctgaagggc agcgccgagg gcagcatcag ataccccgcc tggaacggca tcatcagcca
     9061 ggaggtgctg gactacctga gcagctacat caacaggcgg atctgagctt ctctagctag
     9121 agtcgatcga caagctcgag tttctccata ataatgtgtg agtagttccc agataaggga
     9181 attagggttc ctatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta
     9241 tttgtatttg taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt
     9301 actaaaatcc agatcgctca gatgagaccg cagctggcac gacaggtttc ccgactggaa
     9361 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9421 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9481 cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg tcgactctag
     9541 aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt cgtgactggg
     9601 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9661 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9721 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     9781 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     9841 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     9901 ctgtgacggt ctcagggaca cgaagtgatc cgtttaaact atcagtgttt gacaggatat
     9961 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg
    10021 cgtgaaaagg tttatccgtt cgtccatttg tatgtgccag ccgcctttgc gacgctcacc
    10081 gggctggttg ccctcgccgc tgggctggcg gccgtctatg gccctgcaaa cgcgccagaa
    10141 acgccgtcga agccgtgtgc gagacaccgc ggccgccggc gttgtggata cctcgcggaa
    10201 aacttggccc tcactgacag atgaggggcg gacgttgaca cttgaggggc cgactcaccc
    10261 ggcgcggcgt tgacagatga ggggcaggct cgatttcggc cggcgacgtg gagctggcca
    10321 gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt tcccacagat gatgtggaca
    10381 agcctgggga taagtgccct gcggtattga cacttgaggg gcgcgactac tgacagatga
    10441 ggggcgcgat ccttgacact tgaggggcag agtgctgaca gatgaggggc gcacctattg
    10501 acatttgagg ggctgtccac aggcagaaaa tccagcattt gcaagggttt ccgcccgttt
    10561 ttcggccacc gctaacctgt cttttaacct gcttttaaac caatatttat aaaccttgtt
    10621 tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc cgaagggggg tgccccccct
    10681 tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc ccccaggggc tgcgcccctc
    10741 ggccgcgaac ggcctcaccc caaaaatggc agcgctggcc aattcccgag tgcgcggaac
    10801 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10861 ctgataaatg cttcaataat attgaaaaag gaagagtatg gctaaaatga gaatatcacc
    10921 ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc
    10981 tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag
    11041 ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga
    11101 aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct
    11161 gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc
    11221 tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc
    11281 ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa
    11341 taacgatctg gccgatgtgg attgcgaaaa ctgggaagag gacactccat ttaaagatcc
    11401 gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca
    11461 cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga
    11521 tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgct
    11581 cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa
    11641 gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt agctgtcaga
    11701 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
    11761 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
    11821 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
    11881 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
    11941 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
    12001 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
    12061 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
    12121 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
    12181 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
    12241 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
    12301 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
    12361 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
    12421 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
    12481 cctggcagat cctagatgtg gcgcaacgat gccggcgaca agcaggagcg caccgacttc
    12541 ttccgcatca agtgttttgg ctctcaggcc gaggcccacg gcaagtattt gggcaagggg
    12601 tcgctggtat tcgtgcaggg caagattcgg aataccaagt acgagaagga cggccagacg
    12661 gtctacggga ccgacttcat tgccgataag gtggattatc tggacaccaa ggcaccaggc
    12721 gggtcaaatc aggaataagg gcacattgcc ccggcgtgag tcggggcaat cccgcaagga
    12781 gggtgaatga atcggacgtt tgaccggaag gcatacaggc aagaactgat cgacgcgggg
    12841 ttttccgccg aggatgccga aaccatcgca agccgcaccg tcatgcgtgc gccccgcgaa
    12901 accttccagt ccgtcggctc gatggtccag caagctacgg ccaagatcga gcgcgacagc
    12961 gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg ccgtggagcg ttcgcgtcgt
    13021 cttgaacagg aggcggcagg tttggcgaag tcgatgacca tcgacacgcg aggaactatg
    13081 acgaccaaga agcgaaaaac cgccggcgag gacctggcaa aacaggtcag cgaggccaag
    13141 caggccgcgt tgctgaaaca cacgaagcag cagatcaagg aaatgcagct ttccttgttc
    13201 gatattgcgc cgtggccgga cacgatgcga gcgatgccaa acgacacggc ccgctctgcc
    13261 ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc tgcaaaacaa ggtcattttc
    13321 cacgtcaaca aggacgtgaa gatcacctac accggcgtcg agctgcgggc cgacgatgac
    13381 gaactggtgt ggcagcaggt gttggagtac gcgaagcgca cccctatcgg cgagccgatc
    13441 accttcacgt tctacgagct ttgccaggac ctgggctggt cgatcaatgg ccggtattac
    13501 acgaaggccg aggaatgcct gtcgcgccta caggcgacgg cgatgggctt cacgtccgac
    13561 cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct tccgcgtcct ggaccgtggc
    13621 aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa tcgtcgtgct gtttgctggc
    13681 gaccactaca cgaaattcat atgggagaag taccgcaagc tgtcgccgac ggcccgacgg
    13741 atgttcgact atttcagctc gcaccgggag ccgtacccgc tcaagctgga aaccttccgc
    13801 ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc gcgagcaggt cggcgaagcc
    13861 tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct gggtcaatga tgacctggtg
    13921 cattgcaaac gctagggcct tgtggggtca gttccggctg ggggttcagc agcccctgct
    13981 cggatctgtt ggaccggaca gtagtcatgg ttgatgggct gcctgtatcg agtggtgatt
    14041 ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg
    14101 taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta atgtactggg
    14161 gttgaacact ct
//
