LOCUS       sequence-378256-       12785 bp    DNA     circular SYN 15-APR-2025
DEFINITION  To act as a 1-input state of the OR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP745_L2pV1_OR_B3RT_FRT_Rluc_1IB_lacZ
FEATURES             Location/Qualifiers
     source          1..12785
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      912..1619
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     protein_bind    1620..1653
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          1753..1941
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    2969..3143
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             3531..5183
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(3597..3615)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5203..5403
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     misc_feature    5746..5801
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             5806..5826
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          6604..6792
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     polyA_signal    7754..7928
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    7998..8019
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8034..8039,8040..8057,8058..8064)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8072..8088
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8077..8099
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8096..8112
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8096..8112
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8108..8431
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8125..8181
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8182..8199)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8182..8198)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8191..8213)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8407..8426)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    8563..8587
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      8675..9385
                     /label=oriV
                     /note="incP origin of replication"
     promoter        9406..9510
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9511..10305
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      10476..11064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10965..10984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             11400..12548
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    12694..12718
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac cctgctttaa tgagatatgc gagaagccta tgatcgcatg atatttgctt
      961 tcaattctgt tgtgcacgtt gtaaaaaacc tgagcatgtg tagctcagat ccttaccgcc
     1021 ggtttcggtt cattctaatg aatatatcac ccgttactat cgtattttta tgaataatat
     1081 tctccgttca atttactgat tgtaccctac tacttatatg tacaatatta aaatgaaaac
     1141 aatatattgt gctgaatagg tttatagcga catctatgat agagcgccac aataacaaac
     1201 aattgcgttt tattattaca aatccaattt taaaaaaagc ggcagaaccg gtcaaaccta
     1261 aaagactgat tacataaatc ttattcaaat ttcaaaagtg ccccaggggc tagtatctac
     1321 gacacaccga gcggcgaact aataacgctc actgaaggga actccggttc cccgccggcg
     1381 cgcatgggtg agattccttg aagttgagta ttggccgtcc gctctaccga aagttacggg
     1441 caccattcaa cccggtccag cacggcggcc gggtaaccga cttgctgccc cgagaattat
     1501 gcagcatttt tttggtgtat gtgggcccca aatgaagtgc aggtcaaacc ttgacagtga
     1561 cgacaaatcg ttgggcgggt ccagggcgaa ttttgcgaca acatgtcgag gctcagcagg
     1621 aagttcctat tctctagaaa gtataggaac ttcggttgct taagaataag taattcttaa
     1681 gcaacccaac aaacaacatt acaattacta tttacaatta caatgacttc aaaggtgtat
     1741 gatcctgagc aggtaagttt ctgcttctac ctttgatata tatataataa ttatcattaa
     1801 ttagtagtaa tataatattt caaatatttt tttcaaaata aaagaatgta gtatatagca
     1861 attgcttttc tgtagtttat aagtgtgtat attttaattt ataacttttc taatatatga
     1921 ccaaaatttg ttgatgtgca gagaaaaaga atgataactg gtccacaatg gtgggcaaga
     1981 tgtaaacaga tgaacgtgct tgattctttc attaattact acgattcaga aaagcacgct
     2041 gagaatgcag ttatctttct tcacggaaac gctgcatctt catatttgtg gagacatgtt
     2101 gtgcctcaca ttgaaccagt ggctaggtgt attatcccag atcttatcgg aatgggaaag
     2161 tctggtaaat ctggaaatgg ttcttacaga cttttggatc attataagta cctcacagct
     2221 tggttcgaac ttctcaactt acctaagaag attattttcg ttggacacga ttggggtgct
     2281 tgcttggcat tccattactc atacgaacac caagataaga tcaaagctat agttcatgca
     2341 gagagtgttg tggatgtgat agaatcttgg gatgagtggc cagatattga agaggatatt
     2401 gctttgatca agtctgaaga gggagaaaag atggttctcg aaaataactt tttcgtggaa
     2461 actatgcttc cttcaaagat tatgagaaaa ttggaaccag aagagtttgc tgcatatctc
     2521 gaacctttca aggaaaaagg agaggttaga aggccaacac tcagttggcc tagggaaata
     2581 ccattagtta agggaggtaa acctgatgtt gtgcagatcg tgagaaatta taacgcttac
     2641 cttagggcat cagatgattt gcctaagatg ttcatcgagt cagatccagg atttttcagt
     2701 aatgctatag ttgaaggtgc aaagaaattt ccaaacaccg agttcgttaa ggtgaaagga
     2761 ttgcatttct cacaagaaga tgctcctgat gagatgggaa agtatattaa gtcttttgtt
     2821 gaaagagtgc tcaaaaacga gcagggttct cgatctgatg cttctgaagt aacgtcaaca
     2881 agctcccagt ctgaggtatg cacagtggaa acgccaggtt gtgttggttc ttaagcttct
     2941 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
     3001 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
     3061 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
     3121 aatccagtac taaaatccag atcgctgcaa gaattcaagc ttggagccaa cactcgaatc
     3181 cccacccgtt ggaccaaacc cggctcatta agcgtcggtt cagatttatt tcctttattt
     3241 aaaaaaaagg aaagggtaaa aaatagaaaa ttggaaacag ttaaagccca aaattgtaat
     3301 ttaccgagaa ttgtaaattt acctgaaaac cctacgctat agtttcgact ataaatacca
     3361 aacttaggac ctcacttcag aatcccctcg tcgctgcgtc tctctcccgc aaccttcgat
     3421 tttcgtttat tcgcatccat cggagagaga aaacaatcaa taagcgacct actacaaaca
     3481 agtaagtaag agaaaaacca aaagaagaag agaaacaaca agaagaagta atggaggacg
     3541 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     3601 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     3661 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     3721 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     3781 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     3841 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     3901 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     3961 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     4021 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     4081 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga tctactgggt
     4141 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     4201 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     4261 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     4321 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     4381 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     4441 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     4501 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     4561 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     4621 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     4681 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt tatgtaaaca
     4741 atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct ggagacatag
     4801 cttactggga cgaggacgaa cacttcttca tagttgaccg cttgaagtct ttaattaaat
     4861 acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa caccccaaca
     4921 tcttcgacgc gggcgtggca ggtctgcccg acgatgacgc cggtgaactt cccgccgccg
     4981 ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat tacgtcgcca
     5041 gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac gaagtaccga
     5101 aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata aaggccaaga
     5161 agggcggaaa gtccaaattg taagcttgat cccccgtcga cagctagcta tatcatcaat
     5221 ttatgtatta cacataatat cgcactcagt ctttcatcta cggcaatgta ccagctgata
     5281 taatcagtta ttgaaatatt tctgaattta aacttgcatc aataaattta tgtttttgct
     5341 tggactataa tacctgactt gttattttat caataaatat ttaaactata tttctttcaa
     5401 gatcgctact agaattcgag ctcggagcca acactcgaat ccccacccgt tggaccaaac
     5461 ccggctcatt aagcgtcggt tcagatttat ttcctttatt taaaaaaaag gaaagggtaa
     5521 aaaatagaaa attggaaaca gttaaagccc aaaattgtaa tttaccgaga attgtaaatt
     5581 tacctgaaaa ccctacgcta tagtttcgac tataaatacc aaacttagga cctcacttca
     5641 gaatcccctc gtcgctgcgt ctctctcccg caaccttcga ttttcgttta ttcgcatcca
     5701 tcggagagag aaaacaatca ataagcgacc tactgtattt ttacaacaat taccaacaac
     5761 aacaaacaac aaacaacatt acaattacta tttacaatta caatgcccaa gaaaaagcgg
     5821 aaggtgtcca gctacatgga cctggtggac gacgagcccg ccaccctgta ccacaagttc
     5881 gtggaatgcc tgaaggccgg cgagaacttc tgcggcgata agctgagcgg catcatcacc
     5941 atggccattc tgaaggccat caaggccctg accgaagtga agaaaaccac cttcaacaag
     6001 tacaagacca ccatcaagca gggcctgcag tacgacgtgg gcagcagcac catcagcttc
     6061 gtgtaccacc tgaaggactg cgacgagctg agcagaggcc tgagcgacgc cttcgagccc
     6121 tacaagttca agatcaagag caacaaagag gccaccagct tcaagaccct gttcaggggc
     6181 cctagcttcg gcagccagaa gaactggcgg aagaaagagg tggaccgcga ggtggacaac
     6241 ctgttccaca gcaccgagac agacgagagc atcttcaagt tcatcctgaa caccctggac
     6301 agcatcgaaa cccagaccaa caccgaccgg cagaaaaccg tgctgacctt tatcctgctg
     6361 atgaccttct tcaactgctg ccggaacaac gacctgatga acgtggaccc cagcaccttc
     6421 aagatcgtga agaacaagtt tgtgggctac ctgctgcagg ctgaagtgaa gcagaccaag
     6481 accagaaaga gccggaatat cttcttcttc cccatccggg aaaaccgctt cgacctgttc
     6541 ctggccctgc acgacttctt cagaacctgc cagcccaccc ccaagagcag actgagcgat
     6601 caggtaagtt tctgcttcta cctttgatat atatataata attatcatta attagtagta
     6661 atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc aattgctttt
     6721 ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg accaaaattt
     6781 gttgatgtgc aggtgtccga gcagaagtgg cagctgttcc gggacagcat ggtcatcgac
     6841 tacaaccggt tctttcggaa gttccccgcc agccccatct tcgccattaa gcacggcccc
     6901 aagtcccacc tgggccggca tctgatgaac agctttctgc acaagaacga gctggacagc
     6961 tgggccaaca gcctgggcaa ttggagcagc tcccagaacc agagagagag cggcgccaga
     7021 ctgggctaca cacacggcgg aagagatctg ccccagcccc tgtttggctt cctggccgga
     7081 tactgcgtgc ggaacgaaga gggccacatc gtgggcctgg gcctggaaaa ggacatcaac
     7141 gatctgttcg acggcatcat ggaccccctg aacgagaaag aggacaccga gatctgcgag
     7201 agctacggcg agtgggccaa gattgtgtcc aaggacgtgc tgatcttcct gaagagatac
     7261 cacagcaaga acgcctgtcg gagataccag aacagcaccc tgtatgcccg gaccttcctg
     7321 aaaaccgaga gcgtgaccct gagcggctcc aagggcagcg aggaaccttc tagccctgtg
     7381 cggatcccca tcctgagcat gggaaaggcc agcccctccg agggaagaaa gctgagagcc
     7441 agcgagcacg ccaacgacga caacgagatc gagaagatcg acagcgacag cagccagagc
     7501 gaagagatcc ctatcgagat gagcgactcc gaggacgaga caaccgccag caacatcagc
     7561 ggcatctacc tggacatgag caaggccaac tccaacgtgg tgtacagccc ccctagccag
     7621 acaggcagag ctgctggcgc cggaagaaaa agaggcgtgg gaggcagacg gaccgtggaa
     7681 agcaagcgga gaagagtgct ggcccccatc aaccggtgag cttctctagc tagagtcgat
     7741 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
     7801 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     7861 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     7921 tccagatcgc tttactgaga ccgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     7981 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     8041 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     8101 aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc tagaggatcc
     8161 ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     8221 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     8281 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     8341 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     8401 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8461 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     8521 ggtctcaggg acacgaagtg atccgtttaa actatcagtg tttgacagga tatattggcg
     8581 ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa
     8641 aggtttatcc gttcgtccat ttgtatgtgc cagccgcctt tgcgacgctc accgggctgg
     8701 ttgccctcgc cgctgggctg gcggccgtct atggccctgc aaacgcgcca gaaacgccgt
     8761 cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg atacctcgcg gaaaacttgg
     8821 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     8881 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     8941 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     9001 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     9061 gatccttgac acttgagggg cagagtgctg acagatgagg ggcgcaccta ttgacatttg
     9121 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     9181 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     9241 agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg gggtgccccc ccttctcgaa
     9301 ccctcccggc ccgctaacgc gggcctccca tccccccagg ggctgcgccc ctcggccgcg
     9361 aacggcctca ccccaaaaat ggcagcgctg gccaattccc gagtgcgcgg aacccctatt
     9421 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9481 atgcttcaat aatattgaaa aaggaagagt atggctaaaa tgagaatatc accggaattg
     9541 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     9601 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     9661 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     9721 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     9781 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     9841 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     9901 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
     9961 ctggccgatg tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag
    10021 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
    10081 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
    10141 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gctcagggag
    10201 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
    10261 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt
    10321 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    10381 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    10441 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    10501 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    10561 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    10621 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    10681 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    10741 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    10801 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10861 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10921 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10981 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    11041 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggca
    11101 gatcctagat gtggcgcaac gatgccggcg acaagcagga gcgcaccgac ttcttccgca
    11161 tcaagtgttt tggctctcag gccgaggccc acggcaagta tttgggcaag gggtcgctgg
    11221 tattcgtgca gggcaagatt cggaatacca agtacgagaa ggacggccag acggtctacg
    11281 ggaccgactt cattgccgat aaggtggatt atctggacac caaggcacca ggcgggtcaa
    11341 atcaggaata agggcacatt gccccggcgt gagtcggggc aatcccgcaa ggagggtgaa
    11401 tgaatcggac gtttgaccgg aaggcataca ggcaagaact gatcgacgcg gggttttccg
    11461 ccgaggatgc cgaaaccatc gcaagccgca ccgtcatgcg tgcgccccgc gaaaccttcc
    11521 agtccgtcgg ctcgatggtc cagcaagcta cggccaagat cgagcgcgac agcgtgcaac
    11581 tggctccccc tgccctgccc gcgccatcgg ccgccgtgga gcgttcgcgt cgtcttgaac
    11641 aggaggcggc aggtttggcg aagtcgatga ccatcgacac gcgaggaact atgacgacca
    11701 agaagcgaaa aaccgccggc gaggacctgg caaaacaggt cagcgaggcc aagcaggccg
    11761 cgttgctgaa acacacgaag cagcagatca aggaaatgca gctttccttg ttcgatattg
    11821 cgccgtggcc ggacacgatg cgagcgatgc caaacgacac ggcccgctct gccctgttca
    11881 ccacgcgcaa caagaaaatc ccgcgcgagg cgctgcaaaa caaggtcatt ttccacgtca
    11941 acaaggacgt gaagatcacc tacaccggcg tcgagctgcg ggccgacgat gacgaactgg
    12001 tgtggcagca ggtgttggag tacgcgaagc gcacccctat cggcgagccg atcaccttca
    12061 cgttctacga gctttgccag gacctgggct ggtcgatcaa tggccggtat tacacgaagg
    12121 ccgaggaatg cctgtcgcgc ctacaggcga cggcgatggg cttcacgtcc gaccgcgttg
    12181 ggcacctgga atcggtgtcg ctgctgcacc gcttccgcgt cctggaccgt ggcaagaaaa
    12241 cgtcccgttg ccaggtcctg atcgacgagg aaatcgtcgt gctgtttgct ggcgaccact
    12301 acacgaaatt catatgggag aagtaccgca agctgtcgcc gacggcccga cggatgttcg
    12361 actatttcag ctcgcaccgg gagccgtacc cgctcaagct ggaaaccttc cgcctcatgt
    12421 gcggatcgga ttccacccgc gtgaagaagt ggcgcgagca ggtcggcgaa gcctgcgaag
    12481 agttgcgagg cagcggcctg gtggaacacg cctgggtcaa tgatgacctg gtgcattgca
    12541 aacgctaggg ccttgtgggg tcagttccgg ctgggggttc agcagcccct gctcggatct
    12601 gttggaccgg acagtagtca tggttgatgg gctgcctgta tcgagtggtg attttgtgcc
    12661 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacaa
    12721 attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact ggggttgaac
    12781 actct
//
