LOCUS       sequence-378281-        9553 bp    DNA     circular SYN 15-APR-2025
DEFINITION  To act as the 0-input state of the NOR gate..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9553)
  AUTHORS   Lloyd JPB, Ly F, Gong P, Pflueger J, Swain T, Pflueger C, Fourie E, 
            Khan MA, Kidd BN, Lister R
  TITLE     Synthetic memory circuits for stable cell reprogramming in plants.
  JOURNAL   Nat Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2.
  PUBMED    35788565
REFERENCE   2  (bases 1 to 9553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Jul 4. pii: 10.1038/s41587-022-01383-2. doi: 
            10.1038/s41587-022-01383-2."
COMMENT     Sequence Label: LLP768_L2pV1_NOR_B3RT_FRT_Rluc_0I_lacZ
FEATURES             Location/Qualifiers
     source          1..9553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    845..878
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     intron          978..1166
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     protein_bind    2193..2226
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    2261..2435
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             2823..4475
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(2889..2907)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      4495..4695
                     /label=gene 7 terminator
                     /note="transcription terminator for octopine-type Ti 
                     plasmid gene 7"
     protein_bind    4766..4787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4802..4807,4808..4825,4826..4832)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4840..4856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4845..4867
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4864..4880
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4864..4880
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4876..5199
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    4893..4949
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4950..4967)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4950..4966)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4959..4981)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(5175..5194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    5331..5355
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      5443..6153
                     /label=oriV
                     /note="incP origin of replication"
     promoter        6174..6278
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6279..7073
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7244..7832
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7733..7752
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             8168..9316
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     misc_feature    9462..9486
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gtgccgaatt cggatccgga gtcgacaaaa tttagaacga acttaattat gatctcaaat
       61 acattgatac atatctcatc tagatctagg ttatcattat gtaagaaagt tttgacgaat
      121 atggcacgac aaaatggcta gactcgatgt aattggtatc tcaactcaac attatactta
      181 taccaaacat tagttagaca aaatttaaac aactattttt tatgtatgca agagtcagca
      241 tatgtataat tgattcagaa tcgttttgac gagttcggat gtagtagtag ccattattta
      301 atgtacatac taatcgtgaa tagtgaatat gatgaaacat tgtatcttat tgtataaata
      361 tccataaaca catcatgaaa gacactttct ttcacggtct gaattaatta tgatacaatt
      421 ctaatagaaa acgaattaaa ttacgttgaa ttgtatgaaa tctaattgaa caagccaacc
      481 acgacgacga ctaacgttgc ctggattgac tcggtttaag ttaaccacta aaaaaacgga
      541 gctgtcatgt aacacgcgga tcgagcaggt cacagtcatg aagccatcaa agcaaaagaa
      601 ctaatccaag ggctgagatg attaattagt ttaaaaatta gttaacacga gggaaaaggc
      661 tgtctgacag ccaggtcacg ttatctttac ctgtggtcga aatgattcgt gtctgtcgat
      721 tttaattatt tttttgaaag gccgaaaata aagttgtaag agataaaccc gcctatataa
      781 attcatatat tttcctctcc gctttgaata ctgtattttt acaacaatta ccaacaacaa
      841 caaagaagtt cctattctct agaaagtata ggaacttcgg ttgcttaaga ataagtaatt
      901 cttaagcaac ccaacaaaca acattacaat tactatttac aattacaatg acttcaaagg
      961 tgtatgatcc tgagcaggta agtttctgct tctacctttg atatatatat aataattatc
     1021 attaattagt agtaatataa tatttcaaat atttttttca aaataaaaga atgtagtata
     1081 tagcaattgc ttttctgtag tttataagtg tgtatatttt aatttataac ttttctaata
     1141 tatgaccaaa atttgttgat gtgcagagaa aaagaatgat aactggtcca caatggtggg
     1201 caagatgtaa acagatgaac gtgcttgatt ctttcattaa ttactacgat tcagaaaagc
     1261 acgctgagaa tgcagttatc tttcttcacg gaaacgctgc atcttcatat ttgtggagac
     1321 atgttgtgcc tcacattgaa ccagtggcta ggtgtattat cccagatctt atcggaatgg
     1381 gaaagtctgg taaatctgga aatggttctt acagactttt ggatcattat aagtacctca
     1441 cagcttggtt cgaacttctc aacttaccta agaagattat tttcgttgga cacgattggg
     1501 gtgcttgctt ggcattccat tactcatacg aacaccaaga taagatcaaa gctatagttc
     1561 atgcagagag tgttgtggat gtgatagaat cttgggatga gtggccagat attgaagagg
     1621 atattgcttt gatcaagtct gaagagggag aaaagatggt tctcgaaaat aactttttcg
     1681 tggaaactat gcttccttca aagattatga gaaaattgga accagaagag tttgctgcat
     1741 atctcgaacc tttcaaggaa aaaggagagg ttagaaggcc aacactcagt tggcctaggg
     1801 aaataccatt agttaaggga ggtaaacctg atgttgtgca gatcgtgaga aattataacg
     1861 cttaccttag ggcatcagat gatttgccta agatgttcat cgagtcagat ccaggatttt
     1921 tcagtaatgc tatagttgaa ggtgcaaaga aatttccaaa caccgagttc gttaaggtga
     1981 aaggattgca tttctcacaa gaagatgctc ctgatgagat gggaaagtat attaagtctt
     2041 ttgttgaaag agtgctcaaa aacgagcagg gttctcgatc tgatgcttct gaagtaacgt
     2101 caacaagctc ccagtctgag gtatgcacag tggaaacgcc aggttgtgtt ggttcttaag
     2161 gttgcttaag aataagtaat tcttaagcaa ccgaagttcc tattctctag aaagtatagg
     2221 aacttcgctt ctctagctag agtcgatcga caagctcgag tttctccata ataatgtgtg
     2281 agtagttccc agataaggga attagggttc ctatagggtt tcgctcatgt gttgagcata
     2341 taagaaaccc ttagtatgta tttgtatttg taaaatactt ctatcaataa aatttctaat
     2401 tcctaaaacc aaaatccagt actaaaatcc agatcgctgc aagaattcaa gcttggagcc
     2461 aacactcgaa tccccacccg ttggaccaaa cccggctcat taagcgtcgg ttcagattta
     2521 tttcctttat ttaaaaaaaa ggaaagggta aaaaatagaa aattggaaac agttaaagcc
     2581 caaaattgta atttaccgag aattgtaaat ttacctgaaa accctacgct atagtttcga
     2641 ctataaatac caaacttagg acctcacttc agaatcccct cgtcgctgcg tctctctccc
     2701 gcaaccttcg attttcgttt attcgcatcc atcggagaga gaaaacaatc aataagcgac
     2761 ctactacaaa caagtaagta agagaaaaac caaaagaaga agagaaacaa caagaagaag
     2821 taatggagga cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg
     2881 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     2941 ttgcttttac agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt
     3001 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     3061 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     3121 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca
     3181 tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     3241 aaaaaaaatt accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg
     3301 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     3361 attttgtacc agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg
     3421 gatctactgg gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct
     3481 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     3541 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     3601 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tttacgatcc cttcaggatt
     3661 acaaaattca aagtgcgttg ctagtaccaa ccctattttc attcttcgcc aaaagcactc
     3721 tgattgacaa atacgattta tctaatttac acgaaattgc ttctgggggc gcacctcttt
     3781 cgaaagaagt cggggaagcg gttgcaaaac gcttccatct tccagggata cgacaaggat
     3841 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     3901 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     3961 aaacgctggg cgttaatcag agaggcgaat tatgtgtcag aggacctatg attatgtccg
     4021 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     4081 ctggagacat agcttactgg gacgaggacg aacacttctt catagttgac cgcttgaagt
     4141 ctttaattaa atacaaagga tatcaggtgg cccccgctga attggaatcg atattgttac
     4201 aacaccccaa catcttcgac gcgggcgtgg caggtctgcc cgacgatgac gccggtgaac
     4261 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     4321 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     4381 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     4441 taaaggccaa gaagggcgga aagtccaaat tgtaagcttg atcccccgtc gacagctagc
     4501 tatatcatca atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg
     4561 taccagctga tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt
     4621 tatgtttttg cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta
     4681 tatttctttc aagatcgcta ctatgagacc gcagctggca cgacaggttt cccgactgga
     4741 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     4801 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     4861 acacaggaaa cagctatgac catgattacg ccaagcttgc atgcctgcag gtcgactcta
     4921 gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg
     4981 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     5041 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5101 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     5161 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     5221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     5281 gctgtgacgg tctcagggac acgaagtgat ccgtttaaac tatcagtgtt tgacaggata
     5341 tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat atttaaaagg
     5401 gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcca gccgcctttg cgacgctcac
     5461 cgggctggtt gccctcgccg ctgggctggc ggccgtctat ggccctgcaa acgcgccaga
     5521 aacgccgtcg aagccgtgtg cgagacaccg cggccgccgg cgttgtggat acctcgcgga
     5581 aaacttggcc ctcactgaca gatgaggggc ggacgttgac acttgagggg ccgactcacc
     5641 cggcgcggcg ttgacagatg aggggcaggc tcgatttcgg ccggcgacgt ggagctggcc
     5701 agcctcgcaa atcggcgaaa acgcctgatt ttacgcgagt ttcccacaga tgatgtggac
     5761 aagcctgggg ataagtgccc tgcggtattg acacttgagg ggcgcgacta ctgacagatg
     5821 aggggcgcga tccttgacac ttgaggggca gagtgctgac agatgagggg cgcacctatt
     5881 gacatttgag gggctgtcca caggcagaaa atccagcatt tgcaagggtt tccgcccgtt
     5941 tttcggccac cgctaacctg tcttttaacc tgcttttaaa ccaatattta taaaccttgt
     6001 ttttaaccag ggctgcgccc tgtgcgcgtg accgcgcacg ccgaaggggg gtgccccccc
     6061 ttctcgaacc ctcccggccc gctaacgcgg gcctcccatc cccccagggg ctgcgcccct
     6121 cggccgcgaa cggcctcacc ccaaaaatgg cagcgctggc caattcccga gtgcgcggaa
     6181 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     6241 cctgataaat gcttcaataa tattgaaaaa ggaagagtat ggctaaaatg agaatatcac
     6301 cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc
     6361 ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca
     6421 gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg
     6481 aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc
     6541 tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc
     6601 ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat
     6661 cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga
     6721 ataacgatct ggccgatgtg gattgcgaaa actgggaaga ggacactcca tttaaagatc
     6781 cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc
     6841 acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg
     6901 atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcgc
     6961 tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca
     7021 agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt tagctgtcag
     7081 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7141 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7201 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7261 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7321 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7381 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7441 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7501 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7561 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     7621 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     7681 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7741 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7801 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7861 tcctggcaga tcctagatgt ggcgcaacga tgccggcgac aagcaggagc gcaccgactt
     7921 cttccgcatc aagtgttttg gctctcaggc cgaggcccac ggcaagtatt tgggcaaggg
     7981 gtcgctggta ttcgtgcagg gcaagattcg gaataccaag tacgagaagg acggccagac
     8041 ggtctacggg accgacttca ttgccgataa ggtggattat ctggacacca aggcaccagg
     8101 cgggtcaaat caggaataag ggcacattgc cccggcgtga gtcggggcaa tcccgcaagg
     8161 agggtgaatg aatcggacgt ttgaccggaa ggcatacagg caagaactga tcgacgcggg
     8221 gttttccgcc gaggatgccg aaaccatcgc aagccgcacc gtcatgcgtg cgccccgcga
     8281 aaccttccag tccgtcggct cgatggtcca gcaagctacg gccaagatcg agcgcgacag
     8341 cgtgcaactg gctccccctg ccctgcccgc gccatcggcc gccgtggagc gttcgcgtcg
     8401 tcttgaacag gaggcggcag gtttggcgaa gtcgatgacc atcgacacgc gaggaactat
     8461 gacgaccaag aagcgaaaaa ccgccggcga ggacctggca aaacaggtca gcgaggccaa
     8521 gcaggccgcg ttgctgaaac acacgaagca gcagatcaag gaaatgcagc tttccttgtt
     8581 cgatattgcg ccgtggccgg acacgatgcg agcgatgcca aacgacacgg cccgctctgc
     8641 cctgttcacc acgcgcaaca agaaaatccc gcgcgaggcg ctgcaaaaca aggtcatttt
     8701 ccacgtcaac aaggacgtga agatcaccta caccggcgtc gagctgcggg ccgacgatga
     8761 cgaactggtg tggcagcagg tgttggagta cgcgaagcgc acccctatcg gcgagccgat
     8821 caccttcacg ttctacgagc tttgccagga cctgggctgg tcgatcaatg gccggtatta
     8881 cacgaaggcc gaggaatgcc tgtcgcgcct acaggcgacg gcgatgggct tcacgtccga
     8941 ccgcgttggg cacctggaat cggtgtcgct gctgcaccgc ttccgcgtcc tggaccgtgg
     9001 caagaaaacg tcccgttgcc aggtcctgat cgacgaggaa atcgtcgtgc tgtttgctgg
     9061 cgaccactac acgaaattca tatgggagaa gtaccgcaag ctgtcgccga cggcccgacg
     9121 gatgttcgac tatttcagct cgcaccggga gccgtacccg ctcaagctgg aaaccttccg
     9181 cctcatgtgc ggatcggatt ccacccgcgt gaagaagtgg cgcgagcagg tcggcgaagc
     9241 ctgcgaagag ttgcgaggca gcggcctggt ggaacacgcc tgggtcaatg atgacctggt
     9301 gcattgcaaa cgctagggcc ttgtggggtc agttccggct gggggttcag cagcccctgc
     9361 tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc gagtggtgat
     9421 tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt
     9481 gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg
     9541 ggttgaacac tct
//
