LOCUS       sequence-378562-        9599 bp    DNA     circular SYN 15-APR-2025
DEFINITION  lentiviral transduction of human ZBTB7B gene with a point mutation 
            (R363L) in the first ZF domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR,
            Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, 
            Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi
            HY
  TITLE     Dual targeting of brain region-specific kinases potentiates 
            neurological rescue in Spinocerebellar ataxia type 1.
  JOURNAL   EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. 
            Epub 2021 Mar 11.
  PUBMED    33709453
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2021-04-1"; volume: "40(7):e106106. doi"; pages: " 
            10.15252/embj.2020106106. Epub 2021 Mar 11"
COMMENT     Sequence Label: W118-1_hZBTB7B_R363L
FEATURES             Location/Qualifiers
     source          1..9599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     23..42
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     276..293
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    410..431
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(446..451,452..469,470..476)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     489..511
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     508..524
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     508..524
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     543..563
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        545..563
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        591..817
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             818..998
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1045..1170
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1663..1896
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2081..2125
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2392..2508
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2531..2834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2835..3038
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2989..3009
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3035..3059
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3143..3167
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(3687..3698)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(4794..4818)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4849..5437
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4902..4922)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5320..5331)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5451..5950
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5486..5509)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5883..5902
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5971..6570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5971..5990)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6467..6487
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6708..6941
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7013..7134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7050..7069)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7104..7123
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7174..7309
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7329..7348)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7330..7348)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7358..7375)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7358..7374)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7367..7389)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7516..7971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7603..7622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7813..7834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7997..8101
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8102..8170,8171..8962)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8320..8339)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9133..9599,1..122)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
       61 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      121 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      181 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      241 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      301 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      361 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      421 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      481 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
      541 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
      601 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
      661 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
      721 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
      781 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
      841 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      901 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      961 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     1021 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     1081 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     1141 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     1201 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     1261 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     1321 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     1381 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     1441 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     1501 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1561 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1621 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1681 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1741 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1801 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1861 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1921 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1981 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     2041 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     2101 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     2161 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     2221 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     2281 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     2341 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa cttttaaaag
     2401 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2461 catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat cgataagctt
     2521 gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2581 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2641 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     2701 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2761 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2821 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2881 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2941 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     3001 gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg
     3061 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc tagtccagtg
     3121 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatggggag
     3181 ccccgaggat gacctgattg ggattccatt cccggaccac agcagtgagc tcctgagctg
     3241 cctcaatgag cagcgccagc tgggccacct atgtgacctc accatccgga cgcagggcct
     3301 tgaataccgc acccacaggg ctgtgctagc tgcctgtagc cactacttca agaagctttt
     3361 cactgagggc ggtggcggag ctgtcatggg ggccgggggt agcgggacgg ccactggggg
     3421 agcaggggcc ggtgtgtgtg agctggactt tgtagggcca gaggcactag gcgccctcct
     3481 tgaatttgcc tatacagcca cactgaccac cagcagcgcc aacatgccag ctgtgctcca
     3541 ggctgcccgc ctgctggaga tcccgtgtgt catcgctgct tgcatggaga ttctgcaggg
     3601 cagtgggcta gaagctccca gcccggacga ggatgactgt gagcgagccc gccagtatct
     3661 ggaggccttt gccacagcca cggcctctgg agttcccaat ggtgaagaca gtcctccaca
     3721 ggtgcccctc ccaccacctc cgccaccgcc acctcggcct gttgcccgcc gcagccgcaa
     3781 gccccggaaa gctttcctgc aaaccaaggg ggccagagca aaccacctag tccctgaggt
     3841 gcccacagtg cccgcccatc ccttgaccta tgaggaggag gaggtggcgg gcagagtggg
     3901 cagcagtggg ggcagtgggc cgggggacag ctacagccct cccacaggaa ctgcctcccc
     3961 tcctgagggt ccccagagct acgaacccta tgagggtgag gaagaagaag aggagctggt
     4021 atatccccca gcctatgggc tggcgcaggg tggcgggccc ccgctgtccc cagaggagct
     4081 gggctcagat gaggatgcca tcgatcctga cctgatggcc tacctaagct ccctgcacca
     4141 ggacaacctg gcaccaggcc tggacagcca agacaagctg gtgcgcaaac gccgctccca
     4201 gatgcctcag gagtgccctg tctgccacaa gatcatccat ggggcaggca aactgcctct
     4261 gcacatgagg acccacacag gcgagaagcc ctttgcctgc gaggtctgcg gtgttcgatt
     4321 caccaggaac gacaagctga agatccacat gcggaagcac acgggagagc gcccctactc
     4381 atgcccgcac tgcccagccc gcttcctgca cagctacgac ctcaagaacc acatgcacct
     4441 gcacacaggg gaccggccct atgagtgcca cctgtgccac aaggctttcg ccaaggagga
     4501 ccacctgcag cgccacctca aaggccagaa ctgcctggag gtgcgcaccc gacggcgccg
     4561 caaggacgat gcaccacccc actacccacc accctctacc gctgctgcat cccccgctgg
     4621 cctcgacctc tccaatggcc acctggacac cttccgcctc tctctagctc gattctggga
     4681 gcagtcagcc cccactgggc ccccggtctc taccccaggg ccccctgatg acgatgagga
     4741 ggaaggggca cccaccacac cccaggctga aggtgccatg gagtcctctt aggacccagc
     4801 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg
     4861 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4921 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4981 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     5041 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     5101 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     5161 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     5221 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     5281 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     5341 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     5401 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattctacc gggtagggga
     5461 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     5521 ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc
     5581 tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     5641 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5701 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5761 ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg
     5821 gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc
     5881 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5941 gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc
     6001 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     6061 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     6121 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     6181 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     6241 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     6301 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     6361 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     6421 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     6481 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     6541 tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc
     6601 gaaaggagcg cacgacccca tgcatctcga gggcccggta cctttaagac caatgactta
     6661 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctagc
     6721 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6781 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6841 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6901 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6961 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7021 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7081 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7141 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7201 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7261 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac
     7321 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     7381 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     7441 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     7501 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7561 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7621 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7681 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7741 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7801 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7861 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7921 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     7981 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8041 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8101 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8161 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8221 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8281 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8341 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8401 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8461 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8521 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8581 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     8641 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     8701 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     8761 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     8821 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     8881 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     8941 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9001 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9061 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9121 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9181 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9241 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9301 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9361 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     9421 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     9481 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     9541 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggag
//
