LOCUS       sequence-37865-e         820 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Linda Hicke lab unpublished Ubiquitin constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP2691 - pET30-Ub-K63A
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             32..49
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             59..76
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             83..127
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             158..169
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             170..397
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQAESTLHLVLRLRGG"
     CDS             479..496
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(549..567)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      563..610
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(734..753)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttgtttactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtct
       61 ggtgccacgc ggttctggta tgaaagaaac cgctgctgct aaattcgaac gccagcacat
      121 ggacagccca gatctgggta ccggtggtgg ctccggtatt gagggtcgca tgcagatctt
      181 cgtcaagacg ttaaccggta aaaccataac tctagaagtt gaatcttccg ataccatcga
      241 caacgttaag tcgaaaattc aagacaagga aggcattcca cctgatcaac aaagattgat
      301 ctttgccggt aagcagctcg aggacggtag aacgctgtct gattacaaca ttcaggcgga
      361 gtcgacctta catcttgtct taagactaag aggtggttga ggctctaact ctcctctggc
      421 catggcgata tcggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      481 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      541 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      601 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      661 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      721 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      781 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
//
