LOCUS       sequence-37869-e         931 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  TITLE     Linda Hicke lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LHP1480 - TOPO-TAP-tag
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..264)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             complement(265..438)
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             complement(478..498)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(526..603)
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     complement(687..706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(713..730)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(713..729)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(722..744)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctcggaccac tagtaacggc cgccagtgtg ctggaattcg cccttcccaa tgcggccgcc
       61 ggttgacttc cccgcggaat tcgcgtctac tttcggcgcc tgagcaccat ttagcttttt
      121 agcttctgct aaaaggttag cgctttggct tgggtcatct tttaaacttt ggatgaaggc
      181 gtttcgttgt tcttcgttta agttaggtaa atgtaagatc tcatagaacg cgttttgttg
      241 ttctttgttg aatttgttgt ctactttcgg cgcctgagca tcatttagct ttttagcttc
      301 tgctaaaagg ttagcgcttt ggcttgggtc atcttttaaa ctttggatga aggcgtttcg
      361 ttgttcttcg tttaagttag gtaaatgtaa gatctcatag aacgcgtttt gttgttcttt
      421 gttgaatttg ttgtccacgg cttcatcgtg ttgcgcaaga gccgcggttt tgagctcacc
      481 ctgaaaatac aaattctcgc tagcagtagt tggaatatca taatcaagtg ccccggagga
      541 tgagattttc ttaaagcggt tggctgctga gacggctatg aaattctttt tccatcttct
      601 cttgcggccg cattgggaag ggcgaattct gcagatatcc atcacactgg cggccgctcg
      661 agcatgcatc tagagggccc aattcgccct atagtgagtc gtattacaat tcactggccg
      721 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
      781 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
      841 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
      901 gggtgtggtg gttacgcgca gcgtgaccgc t
//
