LOCUS       sequence-378717-        3622 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#87.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM67
FEATURES             Location/Qualifiers
     source          1..3622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1324..1335
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(1509..1608)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1665..1681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1665..1681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1678..1700)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1853..2441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1933..1952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2577..3392)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2577..3392)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3300..3319
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3384..3403
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     3513..3529
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3513..3529
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      3530..3576
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gatagatttg agccaatacc caacagagct
      301 cgttagaaac ttctcaatca ttgcttacgt tgatcatggc aagttcacac tcgctgatcg
      361 gcttctagaa ctcactggaa ccattaagaa aggccatggc caaccccagt atcttgataa
      421 actgcaggtg gagagagaaa gaggaatcac agttaaagcc cagacagcaa caatgtttta
      481 taagcacagt gtaaatggca atgattgcaa tgatggaaag gaaggaaatg agtcacctaa
      541 gtttttgctg aatcttattg acactcctgg acatgttgat tttagttatg aagtgtcgag
      601 cccgagattg accatgcaca agattgacca gccaacttct gatcctgatt gcgctaaagc
      661 ccaattgaaa tcatatgctg atggatctga ttttgaagct ctaaatcatg caatagagag
      721 gctgacatgc aatgatgcca gtgtctctat tactaaagaa actagcactg ctctaggtct
      781 ggggttcagg tgtgggtttt taggccttct gcacatggat gtttttcatc aacggcttga
      841 acaggagtat ggagctcatg ttatatcaac tgttcctact gtgccttatg tatttgagtt
      901 tcatgatgga agcaagttag aagttcagaa tcccgcttca ttaccctcta atcccaagca
      961 taaagtgaca gcctgttggg aacctacagt gttagctact ataatcattc ctagtgaata
     1021 tgtgggacct gttattaccc ttgtctctga gcgtaggggt cagcaattgg agtactcatt
     1081 cattgacagt caacgggtat tcatgaagta ctgcctgcct ttgagagaaa ttgttgttga
     1141 cttttataat gaattgaaga gtataacatc aggctatgca tcatttgatt atgaggactc
     1201 agattatcaa caagctgata tggtgaaact ggatattctc ttgaacggac aaccagttga
     1261 tgcaatggct accatagttc ataacacgaa agcatatcgt gttgggcgtg aactgacaga
     1321 gaaattgaag ggcgtcttag acaggatttc cgctatgagg aaaaatgtcc ttgctaagtg
     1381 ttatggtggt gatgtttctc gaaaaaggaa gttattggag aagcaaaagg aaggaaagaa
     1441 gagaatgaag cgtgttggtt ccgtcgatgt accacaagaa gcatttcacg aattattgaa
     1501 ggtttctgac ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     1561 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgag gctaggtgga
     1621 ggctcagtga tgataagtct gcgatggtgg atgcatgtgt catggtcata gctgtttcct
     1681 gtgtgaaatt gttatccgct cagagggcac aatcctattc cgcgctatcc gacaatctcc
     1741 aagacattag gtggagttca gttcggcgta tggcatatgt cgctggaaag aacatgtgag
     1801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     2041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     2101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     2161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     2221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     2281 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     2341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2461 ctacggggtc tgacgctcta ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg
     2521 tagggggctt caaatcgtcc gctctgccag tgttacaacc aattaacaaa ttctgattag
     2581 aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca
     2641 tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg
     2701 atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt
     2761 aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa
     2821 tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca
     2881 ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc
     2941 tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc
     3001 aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct
     3061 tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca
     3121 ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt
     3181 ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac
     3241 tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta
     3301 tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc
     3361 gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa
     3421 gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga
     3481 ttttgagaca caacgtggct ttcccccgcc gctctagaac tagtggatcc aaataaaacg
     3541 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgc attatacgag
     3601 acgtccaggt tgggatacct ga
//
