LOCUS       sequence-378791-        4416 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Human wild-type short-form UBASH3A in Gateway pDONR221 (for 
            C-terminal tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Ge Y, Paisie TK, Chen S, Concannon P
  TITLE     UBASH3A Regulates the Synthesis and Dynamics of TCR-CD3 Complexes.
  JOURNAL   J Immunol. 2019 Dec 1;203(11):2827-2836. doi: 
            10.4049/jimmunol.1801338. Epub 2019 Oct 28.
  PUBMED    31659016
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801338"; journalName: "J Immunol"; date: 
            "2019-12-1- 1"; volume: "203"; issue: "11"; pages: "2827-2836"
COMMENT     Sequence Label: pDONR221-WT-short-UBASH3A-Ctag
FEATURES             Location/Qualifiers
     source          1..4416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(126..153)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(245..331)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     380..402
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     394..411
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     395..411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    427..526
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2399..2498)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2515..2534)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2516..2534)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2539..2555)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2539..2555)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2657..2676)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2668..3477
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2735..2754)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3624..4212
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4113..4132
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4366..4383
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
       61 gcgggcagtg agcgcaacgc aattaatacg cgtaccgcta gccaggaaga gtttgtagaa
      121 acgcaaaaag gccatccgtc aggatggcct tctgcttagt ttgatgcctg gcagtttatg
      181 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac aacgttcaaa tccgctcccg
      241 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
      301 agtcttccga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcgtta
      361 acgctagcat ggatgttttc ccagtcacga cgttgtaaaa cgacggccag tcttaagctc
      421 gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
      481 gagcaatgct tttttataat gccaactttg tacaaaaaag caggcttcat ggcagcgggg
      541 gagacgcagc tctacgccaa ggtctccaac aagctcaaga gccgcagcag cccctcgctc
      601 ctggagcccc tcctggccat gggcttcccg gtgcacaccg cgctgaaagc gttggcagcc
      661 acggggagga agacggcgga ggaggccttg gcctggctgc atgatcattg caatgaccct
      721 tccctagacg accccatccc ccaggagtat gcccttttcc tctgtccaac ggggcccctg
      781 ctggaaaaac ttcaagagtt ctggagagag agcaagcgcc agtgtgcaaa gaacagagct
      841 catgaggtct tcccacacgt gacactctgt gacttcttca cgtgtgaaga ccagaaggtg
      901 gaatgcctgt acgaggcgct gaagagagct ggagacaggc tcctgggctc cttccccacg
      961 gccgtgcctc tggctctcca ctcctccatc agctacctcg gcttcttcgt cagtggcagc
     1021 cccgcagacg tcatccggga attcgccatg accttcgcca cggaagcatc tctcttagca
     1081 gactgctccg tgaagccttg caccaaacag ctgcatctga ccttggccca caagttctac
     1141 ccccaccacc agaggacgct ggagcagctg gccagagcca tccccctggg ccacagctgc
     1201 cagtggaccg cagcactcta ctcccgagac atgcgctttg tgcactacca gaccctgaga
     1261 gccctattcc agtacaaacc ccagaacgtg gatgagctga cgctaagtcc tggtgactac
     1321 atctttgtgg accccacgca gcaggacgaa gccagcgagg gctgggtgat tgggatctca
     1381 cagcggacgg gctgccgggg cttcctgccg gaaaactaca cggatcgagc cagtgagtct
     1441 gacacgtggg tgaagcacag gatgtacacc ttcagtctag ccacagacct gaactccaga
     1501 aaggatggtg aagccagcag cagatgcagc ggggaatttc ttccacaaac ggcaaggagt
     1561 cttagcagct tacaggcctt gcaggctacc gttgcaagga agagtgtgct ggtggttcgc
     1621 cacggggaga gagtggatca gatcttcggg aaggcatggc tgcagcaatg ctccactcct
     1681 gatgggaaat actacaggcc agacctgaat ttcccctgca gtctgcccag acggagtcgt
     1741 gggatcaaag actttgaaaa cgatccccca ttatcatcgt gtggcatttt ccagtccaga
     1801 attgcagggg acgcgctact ggacagtggt atcagaatca gctctgtgtt tgcctcccca
     1861 gccctccgct gtgtgcagac ggccaaactc atcctggaag aactcaaact ggagaaaaaa
     1921 atcaagatac gagtggaacc tggaatcttt gaatggacaa aatgggaagc tggcaagacc
     1981 accccaaccc tcatgagcct ggaagagctg aaagaggcaa atttcaacat tgacactgat
     2041 tacaggcccg cgtttcccct gtccgccctc atgccggccg agagctacca ggagtacatg
     2101 gacaggtgca cggcgagcat ggtgcaaatc gtcaacacct gtccgcagga cacgggtgtc
     2161 atcctaattg tgagtcacgg ctccactctg gactcctgca cgcggccact gctcgggctg
     2221 ccgccccggg aatgtgggga ttttgcccaa ctcgtgagaa agatcccttc cctgggcatg
     2281 tgcttctgtg aagaaaataa agaggaagga aaatgggagt tggtgaaccc accggtgaag
     2341 accctgaccc acggggcgaa cgcagcattt aactggagga actggatctc aggcaacaac
     2401 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
     2461 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
     2521 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
     2581 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     2641 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
     2701 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     2761 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
     2821 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     2881 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     2941 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     3001 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     3061 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     3121 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     3181 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     3241 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     3301 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     3361 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     3421 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     3481 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     3541 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     3601 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3661 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3721 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     3781 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3841 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3901 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3961 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4021 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4081 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4141 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4201 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4261 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4321 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4381 gaggaagcgg aagagcgccc aatacgcaaa ccgcct
//
