LOCUS       sequence-379464-       10473 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Nimble cloning-compatible vector for plant ectopic expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Yan P, Tuo D, Shen W, Deng H, Zhou P, Gao X
  TITLE     A Nimble Cloning-compatible vector system for high-throughput gene 
            functional analysis in plants.
  JOURNAL   Plant Commun. 2023 Mar 13;4(2):100471. doi: 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9.
  PUBMED    36352791
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun."; date: "2023-03-13"; volume: "4(2):100471. doi"; pages: " 
            10.1016/j.xplc.2022.100471. Epub 2022 Nov 9"
COMMENT     Sequence Label: pNC-Cam1304-35S
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        181..283
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             314..619
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     498..517
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      707..959
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    981..1005
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2306..2935
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3364..4437
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      4503..4697
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(4933..4955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5041..5181
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5096..5115
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5196..5213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5367..5955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5447..5466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6042..6836)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7261..7285
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    7363..7537
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(7578..8603)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(8670..9346)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    9537..9558
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9573..9578,9579..9596,9597..9603)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9611..9627
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9616..9638
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9635..9651
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9635..9651
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9652..9879
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNRLA
                     AHPPFASWRNSEEARTDRPSQQLRSLNGEC"
     misc_feature    9660..9716
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9720..9737)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9720..9736)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9729..9751)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        10113..10458
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 agtggtctct gtccagtcct ggcctcgtcg gccattctcg actaagttgg cagcatcacc
       61 cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa
      121 tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt
      181 tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt
      241 attttttgag ttatcgagat tttcaggagc taaggaagct aaacttttgc tgacgagaac
      301 agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct
      361 gtttgtggat gtacagagtg atattattga cacgcctggg cgacggatgg tgatccccct
      421 ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat
      481 cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg tatccgttat
      541 cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct
      601 gatgttctgg ggaatataaa tgtcaggctc ccttatacac agggccagtc tggccacttg
      661 tggtctgctg agaccgtgtg aattggtgac cagctcgaat ttccccgatc gttcaaacat
      721 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
      781 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
      841 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
      901 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
      961 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
     1021 gtttattaga ataatcggat atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt
     1081 gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat ccaacccctc
     1141 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacatg
     1201 tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg gcgttttctt
     1261 gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag acattacgcc
     1321 atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac cgacgaccag
     1381 gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct gttttccgag
     1441 aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga ccacctacgc
     1501 cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta
     1561 ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg
     1621 tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc cggcattgcc
     1681 gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc cgccaaggcg
     1741 cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc
     1801 gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat
     1861 cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac cgaggccagg
     1921 cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc ggccgccgag
     1981 aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac gaaccgtttt
     2041 tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc gagccgcccg
     2101 cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg
     2161 cggcctggcc ggcgagcttg gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat
     2221 gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa
     2281 ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg
     2341 gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg ccggggccga
     2401 tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg ccgtgcggga
     2461 agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc
     2521 catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg acttggctgt
     2581 gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc cttacgacat
     2641 atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca cggatggaag
     2701 gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt
     2761 tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca cgcagcgcgt
     2821 gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac ccgagggcga
     2881 cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca tttgagttaa
     2941 tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg tccgagcgca
     3001 cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat gaagcgggtc
     3061 aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt acgccaaggc
     3121 aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta aatgagcaaa
     3181 tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa atcaagaaca
     3241 accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt ggccaggcgt
     3301 aagcggctgg gttgcctgcc ggccctgcaa tggcactgga acccccaagc ccgaggaatc
     3361 ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac
     3421 ctggtggaga agttgaaggc ggcgcaggcc gcccagcggc aacgcatcga ggcagaagca
     3481 cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg
     3541 ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt
     3601 ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc
     3661 gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca
     3721 gacgggcacg tagaggtttc cgcagggccg gcgggcatgg ccagtgtgtg ggattacgac
     3781 ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag
     3841 ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg
     3901 cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc
     3961 acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc
     4021 gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag
     4081 tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg
     4141 gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc
     4201 taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc
     4261 tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg
     4321 atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg
     4381 atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt
     4441 acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt
     4501 cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac
     4561 attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa
     4621 gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc
     4681 cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct
     4741 acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct
     4801 ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg
     4861 ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga
     4921 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     4981 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     5041 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     5101 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     5161 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5341 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     5821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5941 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6001 gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt
     6061 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     6121 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     6181 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     6241 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     6301 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     6361 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     6421 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     6481 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     6541 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     6601 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     6661 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     6721 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     6781 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     6841 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     6901 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     6961 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc
     7021 ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga
     7081 tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat
     7141 gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct
     7201 gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
     7261 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     7321 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
     7381 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
     7441 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
     7501 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
     7561 cccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
     7621 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     7681 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     7741 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     7801 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
     7861 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     7921 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     7981 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     8041 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     8101 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     8161 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     8221 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
     8281 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg aacggcggga gatgcaatag
     8341 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     8401 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     8461 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     8521 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     8581 gcggtgagtt caggcttttt catatctcat tgccccccgg atctgcgaaa gctcgagaga
     8641 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     8701 ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     8761 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     8821 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     8881 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     8941 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     9001 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     9061 ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt gctttgaaga
     9121 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     9181 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     9241 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     9301 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
     9361 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
     9421 ggcaagctgc tctagccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9481 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9541 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9601 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga catgattacg
     9661 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
     9721 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     9781 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     9841 ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag cttggatcag
     9901 attgtcgttt cccgccttca gtttagcttc atggagtcaa agattcaaat agaggaccta
     9961 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
    10021 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
    10081 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
    10141 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
    10201 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
    10261 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
    10321 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta
    10381 agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca
    10441 tttcatttgg agagaacacg ggggactctt gac
//
