LOCUS       sequence-379802-       12667 bp    DNA     circular SYN 14-APR-2025
DEFINITION  episomal expressing human SOX2A61V + KLF4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12667)
  AUTHORS   MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet
            G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, 
            Velychko S
  TITLE     Highly cooperative chimeric super-SOX induces naive pluripotency 
            across species.
  JOURNAL   Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 
            10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
  PUBMED    38141611
REFERENCE   2  (bases 1 to 12667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2023.11.010"; journalName: "Cell Stem Cell"; date: 
            "2024-01-4- 4"; volume: "31"; issue: "1"; pages: "127-147.e9"
COMMENT     Sequence Label: pCXLE-SOX2AV-p2a-KLF4
FEATURES             Location/Qualifiers
     source          1..12667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2704..2760
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    4245..4833
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4298..4318)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4716..4727)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4924..4943)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4989..5044
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5043..5062)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5405..5421)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5405..5421)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5418..5440)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5429..5445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5453..5459,5460..5477,5478..5483))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5498..5519
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    5608..5641
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6102..8027
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGXXGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    8195..8228
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      8415..10204
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(10745..10762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10916..11504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10996..11015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11675..12466,12467..12535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12298..12317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12536..12640)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatgtaca acatgatgga gacggagctg aagccgccgg gcccgcagca aacttcgggg
     1801 ggcggcggcg gcaactccac cgcggcggcg gccggcggca accagaaaaa cagcccggac
     1861 cgcgtcaagc ggcccatgaa tgccttcatg gtgtggtccc gcgggcagcg gcgcaagatg
     1921 gcccaggaga accccaagat gcacaactcg gagatcagca agcgcctggg cgccgagtgg
     1981 aaacttttgt cggagacgga gaagcggccg ttcatcgacg aggctaagcg gctgcgagtg
     2041 ctgcacatga aggagcaccc ggattataaa taccggcccc ggcggaaaac caagacgctc
     2101 atgaagaagg ataagtacac gctgcccggc gggctgctgg cccccggcgg caatagcatg
     2161 gcgagcgggg tcggggtggg cgccggcctg ggcgcgggcg tgaaccagcg catggacagt
     2221 tacgcgcaca tgaacggctg gagcaacggc agctacagca tgatgcagga ccagctgggc
     2281 tacccgcagc acccgggcct caatgcgcac ggcgcagcgc agatgcagcc catgcaccgc
     2341 tacgacgtga gcgccctgca gtacaactcc atgaccagct cgcagaccta catgaacggc
     2401 tcgcccacct acagcatgtc ctactcgcag cagggcaccc ctggcatggc tcttggctcc
     2461 atgggttcgg tggtcaagtc cgaggccagc tccagccccc ctgtggttac ctcttcctcc
     2521 cactccaggg cgccctgcca ggccggggac ctccgggaca tgatcagcat gtatctcccc
     2581 ggcgccgagg tgccggaacc cgccgccccc agcagacttc acatgtccca gcactaccag
     2641 agcggcccgg tgcccggcac ggccattaac ggcacactgc ccctctcaca catgggcacc
     2701 ggtgccacca acttcagcct gctgaagcag gccggcgacg tggaggagaa ccccggcccc
     2761 atgaacaatt cgcccttcac catggctgtc agtgacgcgc tgctcccatc tttctccacg
     2821 ttcgcgtctg gcccggcggg aagggagaag acactgcgtc aagcaggtgc cccgaataac
     2881 cgctggcggg aggagctctc ccacatgaag cgacttcccc cagtgcttcc cggccgcccc
     2941 tatgacctgg cggcggcgac cgtggccaca gacctggaga gcggcggagc cggtgcggct
     3001 tgcggcggta gcaacctggc gcccctacct cggagagaga ccgaggagtt caacgatctc
     3061 ctggacctgg actttattct ctccaattcg ctgacccatc ctccggagtc agtggccgcc
     3121 accgtgtcct cgtcagcgtc agcctcctct tcgtcgtcgc cgtcgagcag cggccctgcc
     3181 agcgcgccct ccacctgcag cttcacctat ccgatccggg ccgggaacga cccgggcgtg
     3241 gcgccgggcg gcacgggcgg aggcctcctc tatggcaggg agtccgctcc ccctccgacg
     3301 gctcccttca acctggcgga catcaacgac gtgagcccct cgggcggctt cgtggccgag
     3361 ctcctgcggc cagaattgga cccggtgtac attccgccgc agcagccgca gccgccaggt
     3421 ggcgggctga tgggcaagtt cgtgctgaag gcgtcgctga gcgcccctgg cagcgagtac
     3481 ggcagcccgt cggtcatcag cgtcagcaaa ggcagccctg acggcagcca cccggtggtg
     3541 gtggcgccct acaacggcgg gccgccgcgc acgtgcccca agatcaagca ggaggcggtc
     3601 tcttcgtgca cccacttggg cgctggaccc cctctcagca atggccaccg gccggctgca
     3661 cacgacttcc ccctggggcg gcagctcccc agcaggacta ccccgaccct gggtcttgag
     3721 gaagtgctga gcagcaggga ctgtcaccct gccctgccgc ttcctcccgg cttccatccc
     3781 cacccggggc ccaattaccc atccttcctg cccgatcaga tgcagccgca agtcccgccg
     3841 ctccattacc aagagctcat gccacccggt tcctgcatgc cagaggagcc caagccaaag
     3901 aggggaagac gatcgtggcc ccggaaaagg accgccaccc acacttgtga ttacgcgggc
     3961 tgcggcaaaa cctacacaaa gagttcccat ctcaaggcac acctgcgaac ccacacaggt
     4021 gagaaacctt accactgtga ctgggacggc tgtggatgga aattcgcccg ctcagatgaa
     4081 ctgaccaggc actaccgtaa acacacgggg caccgcccgt tccagtgcca aaaatgcgac
     4141 cgagcatttt ccaggtcgga ccacctcgcc ttacacatga agagacattt ttaaaagggc
     4201 gaattgcctg caggaattca agcttcgggg actagtcata tgataatcaa cctctggatt
     4261 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4321 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4381 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     4441 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     4501 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     4561 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     4621 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4681 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4741 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4801 cgagtcggat ctccctttgg gccgcctccc cgcatcggta caattcactc ctcaggtgca
     4861 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     4921 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     4981 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     5041 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     5101 ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag
     5161 aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata gaaaagcctt
     5221 gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt taacatccct
     5281 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
     5341 catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc ttggcgtaat
     5401 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5461 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5521 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatctcaa
     5581 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
     5641 ttaggtctga agaggagttt acgtccagcc aagcttagga tcaattctca tgtttgacag
     5701 cttatcatcg ataagctgat cctcacaggc cgcacccagc ttttcttccg ttgccccagt
     5761 agcatctctg tctggtgacc ttgaagagga agaggagggg tcccgagaat ccccatccct
     5821 accgtccagc aaaaaggggg acgaggaatt tgaggcctgg cttgaggctc aggacgcaaa
     5881 tcttgaggat gttcagcggg agttttccgg gctgcgagta attggtgatg aggacgagga
     5941 tggttcggag gatggggaat tttcagacct ggatctgtct gacagcgacc atgaagggga
     6001 tgagggtggg ggggctgttg gagggggcag gagtctgcac tccctgtatt cactgagcgt
     6061 cgtctaataa agatgtctat tgatctcttt tagtgtgaat catgtctgac gaggggccag
     6121 gtacaggacc tggaaatggc ctaggagaga agggagacac atctggacca gaaggctccg
     6181 gcggcagtgg acctcaaaga agagggggtg ataaccatgg acgaggacgg ggaagaggac
     6241 gaggacgagg aggcggaaga ccaggagccc cgggcggctc aggatcaggg ccaagacata
     6301 gagatggtgt ccggagaccc caaaaacgtc caagttgcat tggctgcaaa gggacccacg
     6361 gtggaacagg agcaggagca ggagcgggag gggcaggagc aggaggggca ggagcaggag
     6421 gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggaggg gcaggagcag
     6481 gaggaggggc aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggaggag
     6541 gggcaggagc aggaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
     6601 gaggggcagg agcaggagga ggggcaggag caggaggagg ggcaggaggg gcaggagcag
     6661 gaggaggggc aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggagggg
     6721 caggaggggc aggagsrgsa ggagcaggag gggcaggagc aggaggaggg gcaggagggg
     6781 caggaggggc aggagcagga ggggcaggag caggaggggc aggagcagga ggggcaggag
     6841 caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
     6901 gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggaggg gcaggagggg
     6961 caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     7021 gggcaggagg ggcaggagca ggaggagggg caggagcagg aggggcagga gcaggaggtg
     7081 gaggccgggg tcgaggaggc agtggaggcc ggggtcgagg aggtagtgga ggccggggtc
     7141 gaggaggtag tggaggccgc cggggtagag gacgtgaaag agccaggggg ggaagtcgtg
     7201 aaagagccag ggggagaggt cgtggacgtg gagaaaagag gcccaggagt cccagtagtc
     7261 agtcatcatc atccgggtct ccaccgcgca ggccccctcc aggtagaagg ccatttttcc
     7321 accctgtagg ggaagccgat tattttgaat accaccaaga aggtggccca gatggtgagc
     7381 ctgacgtgcc cccgggagcg atagagcagg gccccgcaga tgacccagga gaaggcccaa
     7441 gcactggacc ccggggtcag ggtgatggag gcaggcgcaa aaaaggaggg tggtttggaa
     7501 agcatcgtgg tcaaggaggt tccaacccga aatttgagaa cattgcagaa ggtttaagag
     7561 ctctcctggc taggagtcac gtagaaagga ctaccgacga aggaacttgg gtcgccggtg
     7621 tgttcgtata tggaggtagt aagacctccc tttacaacct aaggcgagga actgcccttg
     7681 ctattccaca atgtcgtctt acaccattga gtcgtctccc ctttggaatg gcccctggac
     7741 ccggcccaca acctggcccg ctaagggagt ccattgtctg ttatttcatg gtctttttac
     7801 aaactcatat atttgctgag gttttgaagg atgcgattaa ggaccttgtt atgacaaagc
     7861 ccgctcctac ctgcaatatc agggtgactg tgtgcagctt tgacgatgga gtagatttgc
     7921 ctccctggtt tccacctatg gtggaagggg ctgccgcgga gggtgatgac ggagatgacg
     7981 gagatgaagg aggtgatgga gatgagggtg aggaagggca ggagtgatgt aacttgttag
     8041 gagacgccct caatcgtatt aaaagccgtg tattcccccg cactaaagaa taaatcccca
     8101 gtagacatca tgcgtgctgt tggtgtattt ctggccatct gtcttgtcac cattttcgtc
     8161 ctcccaacat ggggcaattg ccggaaccct taatataact tcgtataatg tatgctatac
     8221 gaagttatta ggtccctcga agaggttcac tagcggatct caattgggca tacccatgtt
     8281 gtcacgtcac tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt
     8341 acctggtgag caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga
     8401 atgggagcaa ccagcaggaa aaggacaagc agcgaaaatt cacgccccct tgggaggtgg
     8461 cggcatatgc aaaggatagc actcccactc tactactggg tatcatatgc tgactgtata
     8521 tgcatgagga tagcatatgc tacccggata cagattagga tagcatatac tacccagata
     8581 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata
     8641 taaattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata
     8701 tagattagga tagcctatgc tacccagata tagattagga tagcatatgc tacccagata
     8761 tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc cagatataaa
     8821 ttaggatagc atatactacc ctaatctcta ttaggatagc atatgctacc cggatacaga
     8881 ttaggatagc atatactacc cagatataga ttaggatagc atatgctacc cagatataga
     8941 ttaggatagc ctatgctacc cagatataaa ttaggatagc atatactacc cagatataga
     9001 ttaggatagc atatgctacc cagatataga ttaggatagc ctatgctacc cagatataga
     9061 ttaggatagc atatgctatc cagatatttg ggtagtatat gctacccatg gcaacattag
     9121 cccaccgtgc tctcagcgac ctcgtgaata tgaggaccaa caaccctgtg cttggcgctc
     9181 aggcgcaagt gtgtgtaatt tgtcctccag atcgcagcaa tcgcgcccct atcttggccc
     9241 gcccacctac ttatgcaggt attccccggg gtgccattag tggttttgtg ggcaagtggt
     9301 ttgaccgcag tggttagcgg ggttacaatc agccaagtta ttacaccctt attttacagt
     9361 ccaaaaccgc agggcggcgt gtgggggctg acgcgtgccc ccactccaca atttcaaaaa
     9421 aaagagtggc cacttgtctt tgtttatggg ccccattggc gtggagcccc gtttaatttt
     9481 cgggggtgtt agagacaacc agtggagtcc gctgctgtcg gcgtccactc tctttcccct
     9541 tgttacaaat agagtgtaac aacatggttc acctgtcttg gtccctgcct gggacacatc
     9601 ttaataaccc cagtatcata ttgcactagg attatgtgtt gcccatagcc ataaattcgt
     9661 gtgagatgga catccagtct ttacggcttg tccccacccc atggatttct attgttaaag
     9721 atattcagaa tgtttcattc ctacactagt atttattgcc caaggggttt gtgagggtta
     9781 tattggtgtc atagcacaat gccaccactg aaccccccgt ccaaatttta ttctgggggc
     9841 gtcacctgaa accttgtttt cgagcacctc acatacacct tactgttcac aactcagcag
     9901 ttattctatt agctaaacga aggagaatga agaagcaggc gaagattcag gagagttcac
     9961 tgcccgctcc ttgatcttca gccactgccc ttgtgactaa aatggttcac taccctcgtg
    10021 gaatcctgac cccatgtaaa taaaaccgtg acagctcatg gggtgggaga tatcgctgtt
    10081 ccttaggacc cttttactaa ccctaattcg atagcatatg cttcccgttg ggtaacatat
    10141 gctattgaat tagggttagt ctggatagta tatactacta cccgggaagc atatgctacc
    10201 cgtttagggt taacaagggg gccttataaa cactattgct aatgccctct tgagggtccg
    10261 cttatcggta gctacacagg cccctctgat tgacgttggt gtagcctccc gtagtcttcc
    10321 tgggcccctg ggaggtacat gtcccccagc attggtgtaa gagcttcagc caagagttac
    10381 acataaaggc aatgttgtgt tgcagtccac agactgcaaa gtctgctcca ggatgaaagc
    10441 cactcagtgt tggcaaatgt gcacatccat ttataaggat gtcaactaca gtcagagaac
    10501 ccctttgtgt ttggtccccc cccgtgtcac atgtggaaca gggcccagtt ggcaagttgt
    10561 accaaccaac tgaagggatt acatgcactg ccccgcgaag aaggggcaga gatgtcgtag
    10621 tcaggtttag ttcgtccggg gcggggatcg atcctctaga gtcgacgcta gcggatccgc
    10681 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
    10741 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
    10801 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
    10861 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    10921 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    10981 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    11041 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    11101 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    11161 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    11221 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    11281 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    11341 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    11401 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    11461 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    11521 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    11581 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    11641 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    11701 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    11761 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    11821 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    11881 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    11941 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    12001 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    12061 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    12121 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    12181 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    12241 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    12301 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    12361 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    12421 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    12481 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    12541 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    12601 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    12661 cacctgg
//
