LOCUS       sequence-380233-        8902 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Doxycyline-inducible (TetOn) human Cdt1 (SV40 NLS localization and 
            C-terminal mCherry tag) with mutations which abroggate its S phase 
            degradation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Ratnayeke N, Baris Y, Chung M, Yeeles JTP, Meyer T
  TITLE     CDT1 inhibits CMG helicase in early S phase to separate origin 
            licensing from DNA synthesis.
  JOURNAL   Mol Cell. 2023 Jan 5;83(1):26-42.e13. doi: 
            10.1016/j.molcel.2022.12.004.
  PUBMED    36608667
REFERENCE   2  (bases 1 to 8902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2022.12"; journalName: "Mol Cell"; date: 
            "2023-01-5- 5"; volume: "83"; issue: "1"; pages: "26-42.e13"
COMMENT     Sequence Label: pLV-TetOn-ND-Cdt1-mCherry
FEATURES             Location/Qualifiers
     source          1..8902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     protein_bind    2202..2220
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2237..2255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2273..2291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2309..2327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2344..2362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2380..2398
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2410..2448
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2445..2469
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4104..4124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4137..4847
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4282..4300)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4550..4569
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4871..5459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4924..4944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5342..5353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5666..6299
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6427..6443)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6427..6443)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6440..6462)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6451..6467
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6475..6481,6482..6499,6500..6505))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6520..6541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6658..6675)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6829..7417)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6909..6928)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7588..8379,8380..8448))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8211..8230
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8449..8553)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8601..8735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8638..8657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8692..8711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatagacta gacgagttta ctccctatca gtgatagaga
     2221 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2281 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     2341 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2401 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg caccggcgaa ttcgccacca tgatagctcc gccaaagctc gcttgtcgga
     2521 ctccctctcc ggctagaccg gcacttcgcg cccccgcctc cgctacttca ggtagccgaa
     2581 agagggcgag gccaccagca gcccccggac gcgatcaggc gaggccacct gctcgagcgg
     2641 ctgctcgcct ctcagtagat gaggttagca gccccagtac ccccgaggcc ccagacatcc
     2701 cagcctgccc ttctccgggc cagaagataa agaaatccac cccggcagca ggtcagccgc
     2761 cccacctgac atccgcgcag gaccaggaca ccatctctga gcttgcgtca tgcctgcaac
     2821 gggcccggga gctgggggca agagtccggg cgctgaaggc cagtgcccag gatgctgggg
     2881 agtcctgcac cccagaggcc gagggccgcc ctgaggagcc atgtggcgag aaggcgcccg
     2941 cctaccagcg cttccatgcc ctggcccagc ccggcctgcc gggactcgtg ctgccctaca
     3001 agtaccaggt gctggcggag atgttccgca gcatggacac catcgtgggc atgctccaca
     3061 accgctccga gacgcccacc tttgccaagg tccagcgggg cgtccaggac atgatgcgta
     3121 ggcgttttga ggagcgcaat gttggccaga tcaaaaccgt gtacccggcc tcctaccgct
     3181 tccgccagga gcgcagtgtc cccaccttca aggatggcac caggaggtca gattaccagc
     3241 tcaccatcga gccactgctg gagcaggagg ctgacggagc agccccccag ctcacggcct
     3301 cgcgcctcct gcagcgacgg cagatcttca gccagaagct ggtggagcat gtcaaggagc
     3361 accacaaggc cttcctggcc tccctgagcc ccgccatggt ggtgccggag gaccagctga
     3421 cccgctggca cccgcgcttc aacgtggatg aagtacccga catcgagccg gccgcgctgc
     3481 cccagccacc cgccacggag aagctcacca ctgctcagga ggtgctggcc cgggcccgca
     3541 acctgatttc acccaggatg gagaaggcct tgagtcaatt ggccctgcgc tctgctgcgc
     3601 ccagcagccc cgggtctccc aggccagcac tgccggctac cccaccagcc accccgcctg
     3661 cagcctctcc cagtgctctg aagggggtgt cccaggatct gctggagcgg atccgagcca
     3721 aggaggcaca gaagcagctg gcacagatga cgcggtgccc ggagcaggag cagcggctgc
     3781 agcgcttaga acggctgcct gagctggccc gcgtgctgcg gagcgtcttt gtgtccgaac
     3841 gcaagcctgc gctcagcatg gaggtggcct gtgccaggat ggtgggcagc tgttgtacta
     3901 tcatgagccc tggggaaatg gagaagcacc tgctgctcct ctccgagctg ctgccggact
     3961 ggctcagcct ccaccgcatc cgcaccgaca cctacgtcaa gctggacaag gccgcggacc
     4021 tcgcccacat cactgcacgc ctggcccacc agacacgtgc tgaggagggg ctgggtgcag
     4081 gaagcgctgg gggatccatg gcacctaaga aaaagcggaa agtgggcgga agtggtatgg
     4141 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     4201 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     4261 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     4321 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     4381 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     4441 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     4501 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     4561 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     4621 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     4681 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     4741 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     4801 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgaccc gggcggggcg
     4861 cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4921 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4981 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5041 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5101 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5161 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     5221 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     5281 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     5341 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     5401 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     5461 tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca
     5521 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     5581 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     5641 cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat
     5701 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     5761 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     5821 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     5881 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     5941 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     6001 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     6061 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     6121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     6181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     6241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     6301 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     6361 gagtgagagg ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta
     6421 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     6481 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     6541 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     6601 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6661 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6721 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6781 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6841 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6901 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6961 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     7021 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     7081 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     7141 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     7201 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     7261 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     7321 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     7381 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     7441 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     7501 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     7561 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     7621 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7681 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7741 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7801 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7861 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7921 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7981 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     8041 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     8101 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     8161 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     8221 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     8281 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     8341 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     8401 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     8461 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     8521 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     8581 cgtcgacgga tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca
     8641 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     8701 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
     8761 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
     8821 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
     8881 tatgtactac aaacttagta gt
//
