LOCUS       sequence-380288-        9226 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Constitutive lentiviral expression of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9226)
  AUTHORS   Kim M, Ly SH, Xie Y, Duronio GN, Ford-Roshon D, Hwang JH, Sulahian 
            R, Rennhack JP, So J, Gjoerup O, Talamas JA, Grandclaudon M, Long 
            HW, Doench JG, Sethi NS, Giannakis M, Hahn WC
  TITLE     YAP1 and PRDM14 converge to promote cell survival and tumorigenesis.
  JOURNAL   Dev Cell. 2022 Jan 24;57(2):212-227.e8. doi: 
            10.1016/j.devcel.2021.12.006. Epub 2022 Jan 5.
  PUBMED    34990589
REFERENCE   2  (bases 1 to 9226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.12.006"; journalName: "Dev Cell"; date: 
            "2022-01-24- 24"; volume: "57"; issue: "2"; pages: "212-227.e8"
COMMENT     Sequence Label: pLX317 GFP
FEATURES             Location/Qualifiers
     source          1..9226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        108..1286
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          339..1277
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1234..1254
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1306..1330
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(1351..1353,1354..1356,1357..2067)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1396..1417)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1657..1676)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2004..2025
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2087..2111)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2119..2160
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2271..2859
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2324..2344)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2742..2753)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2931..3164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3242..3363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3279..3298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3333..3352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3403..3538
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3558..3577)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3559..3577)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3587..3604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3587..3603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3596..3618)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3745..4200
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3832..3851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4042..4063
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4226..4330
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4331..4399,4400..5191)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4549..4568)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5362..5950
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5851..5870
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6104..6121
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6238..6259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6274..6279,6280..6297,6298..6304)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6312..6328
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6317..6339
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6336..6352
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6336..6352
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6371..6391
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6373..6391
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6419..6645
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6646..6826
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6873..6998
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7491..7724
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7909..7953
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8184..8394
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8206..8402
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8253..8388
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8434..9036
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8434..8453)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8930..8950
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9079..9196
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga
       61 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
      121 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      181 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      241 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      301 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
      361 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
      421 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
      481 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
      541 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
      601 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
      661 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
      721 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      781 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      841 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      901 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      961 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     1021 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     1081 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     1141 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     1201 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     1261 tttttcttcc atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa
     1321 aaagcaggct ccgcggccgc ccccttcacc atggtgagca agggcgagga gctgttcacc
     1381 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     1441 tctggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1501 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag
     1561 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1621 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1681 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1741 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     1801 gtctatatca tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     1861 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1921 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1981 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2041 actctcggca tggacgagct gtacaagtcc aagggtgggc gcgccgaccc agctttcttg
     2101 tacaaagtgg ttgatatcgg taagcctatc cctaaccctc tcctcggtct cgattctacg
     2161 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2221 ggaaaggacg aatcgatttt gtatttggag gccctacgcg ttaagtcgac aatcaacctc
     2281 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     2341 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     2401 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     2461 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     2521 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     2581 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     2641 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     2701 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     2761 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     2821 ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac
     2881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2941 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc
     3181 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     3241 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3361 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     3421 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3481 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3541 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     3601 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     3661 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3721 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3781 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3841 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3901 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3961 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4021 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     4081 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     4141 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     4201 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4261 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4321 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4381 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4441 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4501 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4561 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4621 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4681 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4741 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4801 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4861 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4921 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4981 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5041 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5101 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     5161 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5221 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5281 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5341 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5401 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5461 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5521 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5581 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5641 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5701 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5761 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5821 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5881 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5941 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6001 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6061 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6121 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     6181 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     6241 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     6301 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     6361 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     6421 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     6481 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     6541 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     6601 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     6661 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     6721 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     6781 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     6841 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     6901 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     6961 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     7021 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     7081 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     7141 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     7201 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     7261 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     7321 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     7381 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     7441 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     7501 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     7561 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     7621 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     7681 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     7741 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     7801 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     7861 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     7921 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     7981 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     8041 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     8101 acccacctcc caaccccgag gggacccatg cattgcatct caattagtca gcaaccaggt
     8161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt ctcaattagt
     8221 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8281 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8341 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8401 aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc cacggtgcgc
     8461 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     8521 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     8581 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     8641 ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     8701 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     8761 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     8821 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     8881 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     8941 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     9001 acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct tgagacaaat
     9061 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     9121 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     9181 aaaaattcaa aattttcggg tttattacag ggacagcaga gatcca
//
