LOCUS       sequence-38034-e         942 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3327 (=HL 716 + pHL 1009)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    33..51
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    58..76
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(97..115)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        183..287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             472..850
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(506..525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tacgcatttt ttttcctcct tagtcgacag tatctctatc actgataggg atgtcaatct
       61 ctatcactga tagggactcg aggtgaagac gaaagggcct cgtgatacgc ctatttttat
      121 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      241 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      901 taatagactg gatggaggcg gataaagttg caggaccact ct
//
