LOCUS       sequence-380403-        2260 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A2 Proximal promoter sequence consisting of the target sequences for
            the gRNA3 (GB1724) and gRNA2 (GB1839) flanked by random sequences. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3274
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     196..215
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     443..461
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(465..508)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    511..532
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      744..801
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(825..842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(996..1584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1076..1095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1767..1861)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1882..2260,1..281))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
       61 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
      121 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
      181 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
      241 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
      301 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
      361 ttggaacctc ttacgtgccc gatcaactcg agtgccacct gacgtctaag aaaccattat
      421 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa
      481 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag agcgatgagt
      541 ggtctcgtga cgctgtatct aatagaatct tcggctatta gtggctgcgg caaaatatct
      601 tctaagcgaa tccagaaacc cgcggctcag tggctcctgt gctcggtggt caaatggtat
      661 cgtggctgcg taatcagccg tatccagcaa cactacgcta tctccctgag accactcatc
      721 gcgactagta gcggccgctg cagtccggca aaaaagggca aggtgtcacc accctgccct
      781 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg
      841 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
      901 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      961 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1021 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1081 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1141 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1201 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1261 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1321 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1381 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     1441 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1501 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1561 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1621 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1681 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1741 acttggtctg acagctcgag gcttggattc tcaccaataa aaaacgcccg gcggcaaccg
     1801 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
     1861 ccaagcgagc tcgatatcaa attacgcccc gccctgccac tcgtcgcagt actgttgtaa
     1921 ttcattaagc attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc
     1981 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     2041 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     2101 ggctgatacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     2161 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     2221 actccagagt gatgaaaacg tttcagtttg ctcatggaaa
//
