LOCUS       sequence-380485-        8260 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AAVS1 targeting construct with the Bxb1 landing pad version 2 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Blanch-Asensio A, van der Vaart B, Vinagre M, Groen E, Arendzen C, 
            Freund C, Geijsen N, Mummery CL, Davis RP
  TITLE     Generation of AAVS1 and CLYBL STRAIGHT-IN v2 acceptor human iPSC 
            lines for integrating DNA payloads.
  JOURNAL   Stem Cell Res. 2023 Feb;66:102991. doi: 10.1016/j.scr.2022.102991. 
            Epub 2022 Dec 5.
  PUBMED    36495703
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2023-02"; volume: "66:102991. doi"; pages: " 
            10.1016/j.scr.2022.102991. Epub 2022 Dec 5"
COMMENT     Sequence Label: AAVS1-Bxb1-LP-v2-TC
FEATURES             Location/Qualifiers
     source          1..8260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    33..836
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     856..872
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(894..926)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        982..1481
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1017..1040)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1414..1433
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      1490..1499
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1496..1498,1499..1501,1502..2215)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(1541..1562)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    2265..2386
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2302..2321)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2356..2375
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2405..2452
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             2458..2829
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="AKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDV
                     TLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGRE
                     FALRDPAGNCVHFVAEEQD"
     primer_bind     complement(2912..2931)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2977..3032
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3031..3050)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     protein_bind    complement(3420..3452)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    3496..4332
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(4366..4385)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4367..4385)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4392..4408)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4546..4848
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4727..4746
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(5251..5270)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5865..5884
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(6245..7036,7037..7105))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6868..6887
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7229..7817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7718..7737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7971..7988
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8105..8126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8141..8146,8147..8164,8165..8171)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8179..8195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8184..8206
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8203..8219
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8203..8219
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8240..8258
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctagtcctgc aggtttaaac gaattcgccc tttgctttct ctgaccagca ttctctcccc
       61 tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac ggctggccca
      121 gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt ccccttgctc
      181 tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg gcgctgcacc
      241 acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc atctctcctc
      301 cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct ccttctgggg
      361 cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc cttgcgtccc
      421 gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc aaagtacccc
      481 gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga caccccgttc
      541 tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct acccccctta
      601 cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca ggggtccgag
      661 agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg acagcatgtt
      721 tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc cagggccggt
      781 taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag tggggcaagg
      841 tccagtcacg acgttgtaaa acgacggcca gtcgctagcg agctcggcgc gccataactt
      901 cgtatagcat acattatacg aagttagaat tcgccttctg caggagcgta cagaacccag
      961 ggccctggca cccgtgctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     1021 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     1081 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     1141 cttccactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     1201 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     1261 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     1321 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     1381 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     1441 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagg ccaccatggt
     1501 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1561 cgtaaacggc cacaagttca gcgtgagggg cgagggcgag ggcgatgcca ccaacggcaa
     1621 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1681 gaccaccctg agccacggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     1741 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1801 ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctagtgaa
     1861 ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1921 ggagtacaac ttcaacagcc acaacatcta tatcatggcc gtcaagcaga agaacggcat
     1981 caaggtgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca
     2041 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca gccactacct
     2101 gagcacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     2161 ggagttccgc accgccgccg ggatcactct cggcatggac gagctgtaca agtaatcgat
     2221 aactgcagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     2281 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     2341 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctagatat
     2401 acgtggtttg tctggtcaac caccgcggtc tcagtggtgt acggtacaaa cccatatgcc
     2461 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     2521 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     2581 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     2641 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     2701 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     2761 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     2821 caggactgac attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2881 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2941 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     3001 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     3061 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     3121 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3181 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3241 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3301 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tgaagatcgg
     3361 atcgctacta gaggatgcac atgtgaccga gggaggagct gcaagaactc ttcctcacga
     3421 taacttcgta taggagactt tatacgaagt taagcgcttc gtgagaccac gaagtggctc
     3481 ttcagtggac gaattactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
     3541 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
     3601 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
     3661 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
     3721 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
     3781 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
     3841 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
     3901 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
     3961 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
     4021 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
     4081 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
     4141 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
     4201 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
     4261 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
     4321 tggctctgct ctaagggcga attcgcggcc gctaaattca attcgcccta tagtgagtcg
     4381 tattacaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4441 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4501 cgcaccgatc gcccttccca acagttgcgc agcctatacg tacggcagtt taaggtttac
     4561 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     4621 acgccggggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     4681 tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     4741 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     4801 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggcatg
     4861 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
     4921 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     4981 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     5041 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     5101 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     5161 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     5221 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     5281 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     5341 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     5401 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     5461 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     5521 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     5581 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     5641 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     5701 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     5761 gcatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5821 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5881 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5941 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
     6001 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6061 ccgcatcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6121 aaatacattc aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc
     6181 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6241 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6301 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6361 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6421 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6481 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6541 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6601 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6661 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6721 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6781 tttctgtgac tggtgaatac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6841 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6901 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6961 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7021 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7081 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7141 agggttattg tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7201 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7261 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7321 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7381 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7441 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7501 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7561 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7621 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7681 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7741 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7801 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7861 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7921 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7981 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8041 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8101 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8161 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8221 atgattacgc caagctcaga attaaccctc actaaaggga
//
