LOCUS       sequence-38059-e        7131 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7131)
  AUTHORS   Znosko WA, Yu S, Thomas K, Molina GA, Li C, Tsang W, Dawid IB, Moon 
            AM, Tsang M
  TITLE     Overlapping functions of Pea3 ETS transcription factors in FGF 
            signaling during zebrafish development.
  JOURNAL   Dev Biol. 2010 Jun 1;342(1):11-25. Epub 2010 Mar 24.
  PUBMED    20346941
REFERENCE   2  (bases 1 to 7131)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol.";
            date: "2010-06-1"; volume: "342(1)"; pages: "11-25. Epub 2010 Mar 
            24"
COMMENT     Sequence Label: pGL3-Dusp6_2KBpromoter
FEATURES             Location/Qualifiers
     source          1..7131
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2401..4053
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2467..2485)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4094..4215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4105..4124)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4159..4178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4463..4480)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4634..5222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4714..4733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5393..6184,6185..6253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6016..6035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6254..6358)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6385..6840
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6472..6491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6682..6703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6971..7019
                     /note="synthetic polyadenylation signal"
     misc_feature    7033..7124
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     7073..7092
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgaggaa ccaccccttg taccagactg
       61 gctacaggcc tgagtgaaaa acacattttg gattaaaaaa ttgtgtactc tgttattatt
      121 ttaacataat aaagtgtttg cacaggcaat aatttactca ctctgatgta tttttttcac
      181 aagatggaag atttaatttt tggactcagt tgatctttgt ttagtataga gcagctttga
      241 gcttaattca cacacaggat tgtcctgtgt acacatgcca catttttcat gtgctctggt
      301 gctaaactat agggtttggc aaaaaaaaaa aaacaaaata taacataaaa tgtcacaaaa
      361 attcaaagta tgactataga ataatggttg gtcttctgct actgcgcaga agttcactta
      421 gacagtgtgt cagtaaaaaa gtaaaaaaaa aattccccat tttcagttaa gcacgaacta
      481 actgtttaaa ttccatttat agtctatcta cataataaca tgtccctaag caatgttttc
      541 tcgcctacgt aatgcaaatt gagcaagctg aatagcattt ggccacttgt tttgagcatt
      601 tctaaacgcc agagaccagg cgacctgacc tttttaacga acactagttt atcagtatgc
      661 aattcaaatg aaccctgttc tactgcaact gatatagcaa ttattaaaac tgtgtggtaa
      721 cgacacgaga aaatattgtc agtgacatct gctttaatgc atttgtgcga atccggtgca
      781 gctgttgagg gatttgtgaa tgattttttt ttaagctggc acacacaaga tgacgttagg
      841 ttcattggag gaaatgaccg aagaggaggc gaggaaacac tggcaattcg catctgctgt
      901 taaactattt tgatggctcc atttatgtgt ttattaggcg atcatttagc gcgcgcttcc
      961 acattcattg aatttggatt gagcaatcga tcatcaaggt aataatctga tgagagcatg
     1021 tgcgctttat tgcgattacg tgatctaatt gtactttcat atgttcgagt tacaagagat
     1081 attagcccac agtaaaatgg gggctaatct aaattaggga ttttttgcag caatacttcg
     1141 gattttactt agcatatttt tcagaagtaa tttaataaca catacaaatt tcatggatat
     1201 tatggttata cagaagcttt ttgaaaataa aatgcaatga tctctttttt acgtgtaatg
     1261 tattgggata tgcctattgt tgttattatt tacaaattgt aatttggcaa atattttctt
     1321 gatttatttg ctcttcagtg tttggacttt cagcagtgaa aaccacactg aactgagcta
     1381 aactgaactt caactctgaa aactgaactg acagtttcaa tttaacagaa cttctctgtt
     1441 aagctgcttt gacactagtg tgcattgtga aatctttata aaataaagat tactttaatt
     1501 ttgatatgtt gtgtaaaact gaaatatgta ttttatttgt gcatacgaca ccaaaaagga
     1561 ctaatctgag acagtatata tccgcgttac actgttttac agtacgtcag ctgcgtacca
     1621 tgctccgcac cgccgctgga cgaaacagaa acacgactaa acgggttcaa gtgtgtaacc
     1681 tgagcacgat gtatatgaac ccctcaccaa ataacactcg cactcctcct tccttcccgt
     1741 gaagcgcctc tcgactgttt gtttgcactc cgctgatccg gagcggaaat tcctttccgt
     1801 ttttgtgaat gacaaactta ttaacaattc atcaacacag tctgttccat ccggccccgt
     1861 ttccaggcga cgatcgctcg gcgcctcctg attggctggc cgacataatg tatccattga
     1921 aacgcctcgc gcttgtatcc attcactagc tcacagtgac agagagcgca tctcggactt
     1981 gtggagcgga ggacgcagtg ttgcattctc caggataaac atcgaaagga aataacaata
     2041 cgaagcattt tgcttttgca atcgacattc aactggatat ctagagcgct tcgtgcgaaa
     2101 atcgacgacg agagaagaga ggttatataa taggaagaca acgtcagtgg ctcgcgcact
     2161 cacaggctag tgtgagtcat tgataaatgc acacagttgc atgaatgggg tggaagtaga
     2221 tcgaggctgc gcttgactct cgaaacaaac aaaaaacgag gattttaatc atctcgacgg
     2281 cgcacaagag actttggcgt gtaaaaagct caatgctcca ggcctgagac gtctacggcc
     2341 tcttgcagcg cgtcttctcc gcagttttaa ggtctcacgg taccgcaccg tatttcaccc
     2401 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
     2461 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
     2521 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
     2581 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     2641 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     2701 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     2761 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     2821 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     2881 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     2941 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     3001 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     3061 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     3121 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     3181 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     3241 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     3301 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     3361 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     3421 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     3481 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     3541 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     3601 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     3661 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     3721 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     3781 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     3841 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     3901 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     3961 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     4021 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     4081 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4141 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4201 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4261 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     4321 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     4381 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     4441 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4501 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4561 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4621 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4681 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4741 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4801 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4861 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4921 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     4981 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5041 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5101 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5161 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5221 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5281 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5341 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5401 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5461 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5521 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5581 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5641 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     5701 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     5761 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     5821 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     5881 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     5941 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6001 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6061 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6121 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6181 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6241 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6301 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6361 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6421 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6481 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6541 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6601 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6661 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6721 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6781 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     6841 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     6901 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
     6961 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     7021 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     7081 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
