LOCUS       sequence-38074-e         750 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Nern A, Pfeiffer BD, Svoboda K, Rubin GM
  TITLE     Multiple new site-specific recombinases for use in manipulating 
            animal genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 9.
  PUBMED    21831835
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Aug 9."
COMMENT     Sequence Label: pJFRC150-20XUAS-IVS-Flp1::PEST
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    47..141
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    175..269
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    303..397
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    431..525
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
ORIGIN
        1 agcgcctcta tttatactcc ggcgctcgac gtcgctagag tctccgctcg gaggacagta
       61 ctccgctcgg aggacagtac tccgctcgga ggacagtact ccgctcggag gacagtactc
      121 cgctcggagg acagtactcc gacctgcagg catgcactag tgctagagtc tccgctcgga
      181 ggacagtact ccgctcggag gacagtactc cgctcggagg acagtactcc gctcggagga
      241 cagtactccg ctcggaggac agtactccga cctgcaggca tgcgctagcg ctagagtctc
      301 cgctcggagg acagtactcc gctcggagga cagtactccg ctcggaggac agtactccgc
      361 tcggaggaca gtactccgct cggaggacag tactccgacc tgcaggcatg ccctagggct
      421 agagtctccg ctcggaggac agtactccgc tcggaggaca gtactccgct cggaggacag
      481 tactccgctc ggaggacagt actccgctcg gaggacagta ctccgacctg caggcatgca
      541 agcttgcggc cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta
      601 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga
      661 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc
      721 cgtgtttcgt aaagtctgga aacgcggaag
//
