LOCUS       sequence-381185-        6362 bp    DNA     circular SYN 14-APR-2025
DEFINITION  AAV-mediated expression of GCaMP8s under the CamKIIa promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6362)
  TITLE     Looger Lab GCaMP8 plasmids
REFERENCE   2  (bases 1 to 6362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AAV-CamKIIa-jGCaMP8s-WPRE
FEATURES             Location/Qualifiers
     source          1..6362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   19..148
                     /label=AAV2 ITR
     promoter        213..1501
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1540..1557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1839..1860
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1966..1987)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2227..2246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    2818..3406
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2871..2891)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3289..3300)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3409..3425)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3410..3426)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3455..3576
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3466..3485)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3520..3539
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3738..3867
                     /label=AAV2 ITR
     rep_origin      complement(4104..4692)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4184..4203)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4863..5654,5655..5723))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5486..5505
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5724..5828)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5854..6309)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5991..6012)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6203..6222
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcttcgctat tacgccagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
       61 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      121 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
      181 tctacgtagc catgctctag gaagatcttt aacattatgg ccttaggtca cttcatctcc
      241 atggggttct tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt
      301 gacctgccca gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc
      361 ttaaattcca tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg
      421 gcagatgacc aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg
      481 cccagccctg ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta
      541 ccttgacggg aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg
      601 gtccctctgg gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg
      661 ctcagtggtg acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt
      721 ctgtttggta cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc
      781 agggagagct ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc
      841 agaggaagca aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta
      901 gcttcccctg taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc
      961 ttcagaggag atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag
     1021 aatgtggcac ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc
     1081 ccgtctgtta aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact
     1141 tgtggactaa gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga
     1201 acagggtcca cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg
     1261 gcaaagagga gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg
     1321 gcaacgggaa cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc
     1381 tcgcgaggct gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc
     1441 cggttctccg tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag
     1501 cgaattcaag ctgctagcaa ggatccaccc gccaccatgc atcatcacca tcatcacacg
     1561 cgtcgcaaga agaccttcaa ggaggtggcc accgccgtga agatcatcgc catgctgatg
     1621 ggcctgaaga tcaacgtcta tatcaaggcc gacaagcaga agaacggcat caaggcgaac
     1681 ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca ctaccagcag
     1741 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcgtggag
     1801 tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1861 accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac cggagggagc
     1921 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1981 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga tgccacctac
     2041 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     2101 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     2161 cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg caccatcttc
     2221 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     2281 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     2341 aagctggagt acaacctgcc ggaccaactg actgaagagc agatcgcaga atacaaagag
     2401 gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
     2461 gtgatgcggt ctctcggaca caaccccaca gaagcagagc tgcaggacat gatcaatgaa
     2521 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     2581 aaaatgaaat acagggacac ggaagaagaa attagagaag cgttcggtgt gtttgataag
     2641 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
     2701 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     2761 caggtaaact acgaagagtt tgtacaaatg atgacagcga agtaaaagct tatcgataat
     2821 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2881 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2941 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3001 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3061 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3121 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3181 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3241 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3301 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3361 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3421 cctcgacccg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3481 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3541 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3601 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3661 tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg
     3721 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3781 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3841 ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga
     3901 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3961 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4021 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4081 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4141 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4201 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4261 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4321 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4381 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4441 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4501 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4561 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4621 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4681 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4741 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4801 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4861 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4921 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4981 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5041 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5101 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5161 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5221 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5281 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5341 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5401 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5461 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5521 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5581 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5641 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5701 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5761 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5821 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5881 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5941 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6001 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6061 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6121 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6181 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6241 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6301 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6361 cc
//
