LOCUS       sequence-381192-        8817 bp    DNA     circular SYN 15-APR-2025
DEFINITION  knockin donor vector of mAID-mCherry2-Hygro to C-terminus of 
            endogenous human eIF3a.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian 
            G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA
  TITLE     eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant 
            regulator of ribosome content.
  JOURNAL   EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. 
            Epub 2023 May 8.
  PUBMED    37155573
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-06-15"; volume: "42(12):e112362. doi"; pages: " 
            10.15252/embj.2022112362. Epub 2023 May 8"
COMMENT     Sequence Label: eIF3a- pMK293 (mAID-mCherry2-Hygro) plasmid
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    66..87
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(102..107,108..125,126..132)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    140..156
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     145..167
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     164..180
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     164..180
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        198..216
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     198..215
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    complement(1185..1218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(1781..2800)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             complement(1781..2800)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     promoter        complement(2861..3348)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2909..2928)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     3290..3313
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    complement(3409..3442)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3473..3594
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3484..3503)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3538..3557
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(3712..4422)
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4259..4277
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(4429..4632)
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     primer_bind     complement(5664..5683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5665..5683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5690..5707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5690..5706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5699..5721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             5844..6146
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6025..6044
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             6495..7289
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6495..7289
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6549..6568)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7159..7178
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     regulatory      7489..7498
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7495..7869
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     rep_origin      8007..8595
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8496..8515
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8749..8766
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
       61 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
      121 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
      181 catgattacg ccaagctatt taggtgacac tatagaatac tcaagctatg catcaagctt
      241 ggtaccacta gtaacggccg ccagtgtgct ggaattcgcc cttattacta caaattactt
      301 gttgaaagaa tctatcttca aaagaaagaa aattaggaaa gagaatttaa acgattcctc
      361 caaaccatac atttataaat attgtcatat ttaaaatgct tgaaaggcat gaattctcca
      421 ttaatggaaa agcagtttgc tattggcaac agaaaaaaac tcagcgataa tttaccaaat
      481 gctttaaaat ttaaaagcta tactgtttgg attcacactt gcgcagtaat cttgtcatta
      541 caacaactgc aaattgcttt gtactcacct tctctcaagt aaaatctaaa gaaaacccca
      601 ccgtatccaa tgtttgtaat gtttacttca agtgaatcta aaaaatttct aaatttagtt
      661 tatggtaaat acctcaaaat acctaaaata ggtacgtatc aaccacttaa gggtgtgaaa
      721 attgtttcag attcactgct ttaaagggtc ttcaaatata ttttaacaga accttacttc
      781 attccaaatt attatcagat atttcaaaat caaaaaaaaa gtatcagttc tataccaaga
      841 taaaggtgtt actcaatacc tgaagggtca cattttaaat ttatttttta gtttttcttt
      901 tttgtcaaca aatgattgat gaaataatgc aacaccctta aattccagaa aatggcatgg
      961 tttttccaac ctcctgtgga tatggtgcat gatcaatcta ttatatagga ttaatacaag
     1021 ttcatgcttt ttgtgttatg gtgaaacaac attaaagaat ccaatttaga ttggttgaag
     1081 cacaagtata ataatccttg aatgtgatca aacctattta agacaccagt ttaaatccat
     1141 tatcttgaga cttaggatcc tagtgaacct cttcgaggga cctaataact tcgtatagca
     1201 tacattatac gaagttatat taagggttcc ggatctcgac cagcttctga tggaattaga
     1261 acttggcaaa acaatactga gaatgaagtg tatgtggaac agaggctgct gatctcgttc
     1321 ttcaggctat gaaactgaca catttggaaa ccacagtact tagaaccaca aagtgggaat
     1381 caagagaaaa acaatgatcc cacgagagat ctatagatct atagatcatg agtgggagga
     1441 atgagctggc ccttaatttg gttttgcttg tttaaattat gatatccaac tatgaaacat
     1501 tatcataaag caatagtaaa gagccttcag taaagagcag gcatttatct aatcccaccc
     1561 cacccccacc cccgtagctc caatccttcc attcaaaatg taggtactct gttctcaccc
     1621 ttcttaacaa agtatgacag gaaaaacttc cattttagtg gacatcttta ttgtttaata
     1681 gatcatcaat ttctgcatcc cggggatctg atatcatcga tgcatggggt cgtgcgctcc
     1741 tttcggtcgg gcgctgcggg tcgtggggcg ggcggctagc ctattccttt gccctcggac
     1801 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     1861 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     1921 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     1981 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     2041 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     2101 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     2161 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     2221 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     2281 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     2341 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     2401 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     2461 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     2521 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     2581 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     2641 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     2701 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     2761 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tccggatcta tccatgctag
     2821 cggtaagctt cagctgctcg agatctagat ggatgcaggt cgaaaggccc ggagatgagg
     2881 aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg
     2941 aggaccttcg ggcgcccgcc ccgcccctga gcccgccccc ggacccaccc cttcccagcc
     3001 tctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc
     3061 gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca
     3121 tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag
     3181 gagtagaagg tggcgcgaag gggccaccaa agaacggagt cggttggcgc ctaccggtgg
     3241 atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg
     3301 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaatttcg
     3361 aggtcgagat cctaagcttg gctggacgta aactcctctt cagacctaat aacttcgtat
     3421 agcatacatt atacgaagtt atattaaggg ttattgaata tgatcgacgc gttaagatac
     3481 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3541 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3601 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     3661 aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag attacttgta
     3721 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
     3781 ggtgtagtcc tcgttgtggg aaaggatgtc caacttgatg tcgacgttgt aggcgccggg
     3841 cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc
     3901 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
     3961 ccgctcagtg gaggcctccc agcccatggt acgacactgc attacggggc cgtcggaggg
     4021 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
     4081 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc aattgaagcc
     4141 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctt
     4201 ggagccgtac atgaactgag gggacaggat gtcccaggcg aagggcaggg ggccaccctt
     4261 ggtcaccttc agcttggcgg tctgggtgcc ctcgtagggg cggccctcgc cctcgccctc
     4321 gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc gcatgaactc
     4381 cttgatgatg gccatgttat cctcctcgcc cttgctcacc atgctagctt tatacatcct
     4441 caaatcgatt ttcctcaagt acggtgctcc gtccattgat accttcacga acgccgccgc
     4501 ctccgggcca ccgcttgatt tttggcagga aaccatcacg ttcttccggt atgatctcac
     4561 cggtggccat cccacaactt gtgccttggc cggaggtttg gctggatctt taggacaagc
     4621 actcttctcc ttggcgcctg caccggatcc tcctcctcct cctccacgtc gtactgtggt
     4681 ccagccgtct tcatcagtct catttttagt acgacggaga gattccctat ctttctcagc
     4741 tctccatgag gccttctctt tttcaccttc tctttctcgg tctcggtctc tttctcggtc
     4801 tcttgaaaga gctgggggag gaactcgacg aggagggtcc cgctcttcca cccggtcatc
     4861 tttcctgtca tcagcacgtc tccaagaact tactaaaaag tttaattaaa aaaaaaaaat
     4921 taagttagta ctatattcta ttggctcaaa ttactcaaaa ttcctgtact aaagattgtt
     4981 aaatacagaa atcattgtta aatacagaaa ttctgttaaa ttctgtattg ttaaatacag
     5041 aaatcataat gaaagctcat acaatttctg acaatcttta acatacaaat gtacagataa
     5101 aactcaaaat ctgaaaacag gcagaaacca ctagcctggg aaggactaca gttagtataa
     5161 acccgtaaga caaggtggca aaaatctggc tgaatgaaaa ttttataact gaaaggattt
     5221 ttggctcttg aagaatttta acagcaaatt aaccatacaa aatacaggag tttttaaagt
     5281 tatggctatg agagaaaaat tctctcataa caggatgtca agacatatgg aaaattaatt
     5341 tttaaaaacc aagagaaaaa tgaggatttt aaaaatgaaa aatacatata ttttaaaaca
     5401 gaataaactt ctgttacaat ttcagagaaa atgtttctac tttgtgctta atctgccagg
     5461 aagctgcttt taaataacaa tttttttcat ttttattaag taaactttat atatattgcc
     5521 tgctatccaa tttagtctac tactgtactt tgaatttaga gataagtgca ctttacaaca
     5581 tgaccattga gtaaaagggc gaattctgca gatatccatc acactggcgg ccgctcgagc
     5641 atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
     5701 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     5761 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     5821 agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     5881 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     5941 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     6001 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     6061 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     6121 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     6181 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     6241 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     6301 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     6361 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     6421 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     6481 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     6541 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     6601 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     6661 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     6721 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     6781 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     6841 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     6901 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     6961 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     7021 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     7081 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     7141 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     7201 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     7261 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     7321 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     7381 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     7441 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc
     7501 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     7561 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     7621 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     7681 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     7741 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     7801 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     7861 caggactgac acgtgctaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     7921 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     7981 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     8041 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     8101 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     8161 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     8221 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     8281 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     8341 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     8401 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     8461 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     8521 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     8581 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     8641 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     8701 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     8761 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccg
//
