LOCUS       sequence-381826-        3763 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synGR Part (Part 3).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS157
FEATURES             Location/Qualifiers
     source          1..3763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2129..2163)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2234..2893)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2808..2827
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3075..3663
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3564..3583
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtcggtctca tatgctcgag aaaaaaatgg agcgcccctt ccagtgtcgc atttgcatgc
       61 ggaacttttc gagaaatttc attttgcaaa gacatacccg tactcatacc ggtgaaaaac
      121 cgtttcagtg tcggatctgt atgcgaaatt tctccgatag agctaatttg agaagacatc
      181 tacgtacgca caccggcgag aagccattcc aatgccgaat atgcatgcgc aacttcagta
      241 gacatgatca attgactaga cacctaaaaa cccacctgag aggagatggt gctggattga
      301 ttgatatcga taacctggaa gctcgaaaaa caaagaaaaa aataaaagga attcagcagg
      361 ccactacagg agtctcacaa gaaacctctg aaaatcctgg taacaaaaca atagttcctg
      421 caacgttacc acaactcacc cctaccctgg tgtcactgtt ggaggttatt gaacctgaag
      481 tgttatatgc aggatatgat agctctgttc cagactcaac ttggaggatc atgactacgc
      541 tcaacatgtt aggagggcgg caagtgattg cagcagtgaa atgggcaaag gcaataccag
      601 gtttcaggaa cttacacctg gatgaccaaa tgaccctact gcagtactcc tggatgtttc
      661 ttatggcatt tgctctgggg tggagatcat atagacaacc aagtgcaaac ctgctgtgtt
      721 ttgctcctga tctgattatt aatgagcaga gaatgactct accctgcatg tacgaccaat
      781 gtaaacacat gctgtatgtt tcctctgagt tacacaggct tcaggtatct tatgaagagt
      841 atctctgtat gaaaacctta ctgcttctct cttcagttcc taaggacggt ctgaagagcc
      901 aagagctatt tgatgaaatt agaatgacct acatcaaaga gctaggaaaa gccattgtca
      961 agagggaagg aaactccagc cagaactggc agcggtttta tcaactgaca aaactcttgg
     1021 attctatgca tgaagtggtt gaaaatctcc ttaactattg cttccaaaca tttttggata
     1081 agaccatgag tattgaattc cccgagatgt tagctgaaat catcaccaat cagataccaa
     1141 aatactcaaa cggaaatatc aaaaaacttc tgtttcatca aaagggcgac ggagcaggct
     1201 taataaatgc tagcatgacg gtcgaccatg atttcaatag cgaagatatt ttattcccca
     1261 tagaaagcat gagtagtata caatacgtgg agaataataa cccaaataat attaacaacg
     1321 atgttatccc gtattctcta gatatcaaaa acactgtctt agatagtgcg gatctcaatg
     1381 acattcaaaa tcaagaaact tcactgaatt tggggcttcc tccactatct ttcgactctc
     1441 cactgcccgt aacggaaacg ataccatcca ctaccgataa cagcttgcat ttgaaagctg
     1501 atagcaacaa aaatcgcgat gcaagaacta ttgaaaatga tagtgaaatt aagagtacta
     1561 ataatgctag tggctctggg gcaaatcaat acacaactct tacttcacct tatcctatga
     1621 acgacatttt gtacaacatg aacaatccgt tacaatcacc gtcaccttca tcggtacctc
     1681 aaaatccgac tataaatcct cccataaata cagcaagtaa cgaaactaat ttatcgcctc
     1741 aaacttcaaa tggtaatgaa actcttatat ctccacgagc ccaacaacat acgtccatta
     1801 aagataatcg tctgtcctta cctaatggtg ctaattcgaa tcttttcatt gacactaacc
     1861 caaacaattt gaacgaaaaa ctaagaaatc aattgaactc agatacaaat tcatattcta
     1921 actccatttc taattcaaac tccaattcta cgggtaattt aaattccagt tattttaatt
     1981 cactgaacat agactccatg ctagatgatt acgtttctag tgatctctta ttgaatgatg
     2041 atgatgatga cactaattta tcacgccgaa gatttagcga cgttataaca aaccaagagc
     2101 tctaagggcc catcctgaga ccagaccaat aaaaaacgcc cggcggcaac cgagcgttct
     2161 gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga gtccaagcga
     2221 gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt aattcattaa
     2281 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     2341 tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg gcgaagaagt
     2401 tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga ttggctgaaa
     2461 cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca ccgtaacacg
     2521 ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat tcactccaga
     2581 gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga acactatccc
     2641 atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca ttcatcaggc
     2701 gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt acggtcttta
     2761 aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca actgactgaa
     2821 atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta tatccagtga
     2881 tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca aaaaatacgc
     2941 ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc ccgatcaatc
     3001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3061 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3121 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     3181 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     3241 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     3301 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     3361 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     3421 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     3481 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     3541 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     3601 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     3661 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     3721 atgttctttc ctgcgttatc ccctgattct gtggataacc gta
//
