LOCUS       sequence-381840-        5369 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synMR Cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Sanford A, Kiriakov S, Khalil AS
  TITLE     A Toolkit for Precise, Multigene Control in Saccharomyces 
            cerevisiae.
  JOURNAL   ACS Synth Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423.
  PUBMED    36367334
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2022 Nov 11. doi: 10.1021/acssynbio.2c00423."
COMMENT     Sequence Label: pAS163
FEATURES             Location/Qualifiers
     source          1..5369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        478..891
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      3108..3295
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3163..3187)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3612..4403,4404..4472))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4235..4254
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4473..4577)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4680..5268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5169..5188
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccgcccct gaattcgcat ctagaacggg gtcatcacgg ctcatcatgc gccaaacaaa
       61 tgtgtgcaat acacgctcgg atgactgcat gatgaccgca ctgactgggg acagcagatc
      121 cacctaagcc tgtgagagaa gcagacaccc gacagatcaa ggcagttacg tctcaccaaa
      181 ccagatgtca acacagctac aacgccttgc caacagggag ttcttcagag acatggaggc
      241 tcaaaacgaa attattgaca gcctagacat caatagtcat acaacagaaa gcgaccaccc
      301 aactttggct gataatagcg tataaacaat gcatactttg tacgttcaaa atacaatgca
      361 gtagatatat ttatgcatat tacatataat acatatcaca taggaagcaa caggcgcgtt
      421 ggacttttaa ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc cacaagtgat
      481 cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc agattttctc
      541 ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact aaatttcccc
      601 tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa agaaaaaaga
      661 caccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat cacgtttctt
      721 tttcttgaaa attttttttt ttgatttttt tctctttcga tgacctccca ttgatattta
      781 agttaataaa cggtcatcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa
      841 ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa gttttaatta
      901 caaaagatct atgctcgaga aaaaaatggg tacccgccca tatgcttgcc ctgtcgagtc
      961 ctgcgatcgc cgcttttctc gctcggatga gcttacccgc catatccgca tccataccgg
     1021 tcagaagccc ttccagtgtc gaatctgcat gcgtaacttc agtcgtagtg accaccttac
     1081 cacccacatc cgcacccaca caggcgagaa gccttttgcc tgtgacattt gtgggaggaa
     1141 gtttgccagg agtgatgaac gcaagaggca taccaaaatc catacaggtg gagatggtgc
     1201 tggattgatt gatatcgata atttaggagc acgaaagtca aagaagttgg gaaagttaaa
     1261 agggattcac gaggagcagc cacagcagca gcagccccca cccccacccc cacccccgca
     1321 aagcccagag gaagggacaa cgtacatcgc tcctgcaaaa gaaccctcgg tcaacacagc
     1381 actggttcct cagctctcca caatctcacg agcgctcaca ccttcccccg ttatggtcct
     1441 tgaaaacatt gaacctgaaa ttgtatatgc aggctatgac agctcaaaac cagatacagc
     1501 cgaaaatctg ctctccacgc tcaaccgctt agcaggcaaa cagatgatcc aagtcgtgaa
     1561 gtgggcaaag gtacttccag gatttaaaaa cttgcctctt gaggaccaaa ttaccctaat
     1621 ccggtattct tggatgtgtc tatcatcatt tgccttgagc tggagatcgt acaaacatac
     1681 gaacagccaa tttctctatt ttgcaccaga cctagtcttt aatgaagaga agatgcatca
     1741 gtctgccatg tatgaactat gccaggggat gcaccaaatc agccttcagt tcgttcgact
     1801 gcagctcacc tttgaagaat acaccatcat gaaagttttg ctgctactaa gcacaattcc
     1861 aaaggatggc ctcaaaagcc aggctgcatt tgaagaaatg aggacaaatt acatcaaaga
     1921 actgaggaag atggtaacta agtgtcccaa caattctggg cagagctggc agaggttcta
     1981 ccaactgacc aagctgctgg actccatgca tgacctggtg agcgacctgc tggaattctg
     2041 cttctacacc ttccgagagt cccatgcgct gaaggtagag ttccccgcaa tgctggtgga
     2101 gatcatcagc gaccagctgc ccaaggtgga gtcggggaac gccaagccgc tctacttcca
     2161 ccggaagggc gacggagcag gcttaataaa tgctagcatg acggtcgacc atgatttcaa
     2221 tagcgaagat attttattcc ccatagaaag catgagtagt atacaatacg tggagaataa
     2281 taacccaaat aatattaaca acgatgttat cccgtattct ctagatatca aaaacactgt
     2341 cttagatagt gcggatctca atgacattca aaatcaagaa acttcactga atttggggct
     2401 tcctccacta tctttcgact ctccactgcc cgtaacggaa acgataccat ccactaccga
     2461 taacagcttg catttgaaag ctgatagcaa caaaaatcgc gatgcaagaa ctattgaaaa
     2521 tgatagtgaa attaagagta ctaataatgc tagtggctct ggggcaaatc aatacacaac
     2581 tcttacttca ccttatccta tgaacgacat tttgtacaac atgaacaatc cgttacaatc
     2641 accgtcacct tcatcggtac ctcaaaatcc gactataaat cctcccataa atacagcaag
     2701 taacgaaact aatttatcgc ctcaaacttc aaatggtaat gaaactctta tatctccacg
     2761 agcccaacaa catacgtcca ttaaagataa tcgtctgtcc ttacctaatg gtgctaattc
     2821 gaatcttttc attgacacta acccaaacaa tttgaacgaa aaactaagaa atcaattgaa
     2881 ctcagataca aattcatatt ctaactccat ttctaattca aactccaatt ctacgggtaa
     2941 tttaaattcc agttatttta attcactgaa catagactcc atgctagatg attacgtttc
     3001 tagtgatctc ttattgaatg atgatgatga tgacactaat ttatcacgcc gaagatttag
     3061 cgacgttata acaaaccaag agctctaagg gcccatccta actcgaggcg aatttcttat
     3121 gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac aaattttaaa
     3181 gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc ctgtaggtca
     3241 ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct accggcatgc
     3301 cgagcaaatg cctgcaaatc gctccccatt tcgctgagca tgagacggaa atctgctcgt
     3361 cagtggtgct cacactgacg aatcatgtac agatcatacc gatgactgcc tggcgactca
     3421 caactaagca agacagccgg aaccagcgcc ggcgaacacc actgcatata tggcatatca
     3481 caacagtcca actagtgcac tgcagtacag cggccgcgat tatcaaaaag gatcttcacc
     3541 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3601 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3661 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     3721 ccatctggcc ccagtgctgc aatgataccg cgggacccac gctcaccggc tccagattta
     3781 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3841 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3901 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     3961 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4021 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4081 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4141 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4201 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4261 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4321 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4381 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4441 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4501 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4561 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtcatgac caaaatccct
     4621 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4681 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4741 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4801 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     4861 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     4921 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     4981 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5041 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5101 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5161 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5221 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5281 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5341 ttatcccctg attctgtgga taaccgtgc
//
