LOCUS       sequence-38215-e         827 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Valastyan JS, Lindquist S
  TITLE     TorsinA and the TorsinA-Interacting Protein Printor Have No Impact 
            on Endoplasmic Reticulum Stress or Protein Trafficking in Yeast.
  JOURNAL   PLoS One. 2011;6(7):e22744. Epub 2011 Jul 26.
  PUBMED    21818381
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(7)"; pages: "e22744. Epub 2011 Jul 26"
COMMENT     Sequence Label: 413-Met25 Kar2 TorsinADE HDEL
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(32..279)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     245..263
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     281..297
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     282..298
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    331..355
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggc cgcaaattaa agccttcgag cgtcccaaaa
       61 ccttctcaag caaggttttc agtataatgt tacatgcgta cacgcgtctg tacagaaaaa
      121 aaagaaaaat ttgaaatata aataacgttc ttaatactaa cataactata aaaaaataaa
      181 tagggaccta gacttcaggt tgtctaactc cttccttttc ggttagagcg gatgtggggg
      241 gagggcgtga atgtaagcgt gacataacta attacatgac tcgaggtcga cggtatcgat
      301 aagcttgata tcgaattcct gcagcccatc accactttgt acaagaaagc tgggtttcac
      361 aattcatcat gatcatcgta gtaataatct aacttggtga acaccgtttt gcagccttta
      421 tctgagaaaa ctctctcctc tttggggaaa aatgtcatct cagccactct gcttacaatg
      481 tcttcatcaa tttcatagcc tcgggactgc atttccactc ggatacacat ttttaggtgt
      541 ttgtattcca gggggaggaa gggaacaaaa taatcaatga ggttccggtc aattaagctg
      601 ctgtgccaga agccactgtt cttgttattg aaaaccgaca cagacaacgc gtgttcaatg
      661 tctttgagct tgatgtcttc cctctgcttt ccactcctcc agaaatccaa agccacatct
      721 gtgatccttt ctgctccagc attgctgaga aatatgaaca tggctttctg gtaggagacc
      781 ccatccacca ggtcataata gtcgaggaaa ggcttgatgg catctat
//
