LOCUS       sequence-382397-       10547 bp    DNA     circular SYN 15-APR-2025
DEFINITION  doxycycline-inducible expression of mCherry fused to mouse Gata2 in 
            mammalian cell.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Silverio-Alves R, Kurochkin I, Rydstrom A, Vazquez Echegaray C, 
            Haider J, Nicholls M, Rode C, Thelaus L, Lindgren AY, Ferreira AG, 
            Brandao R, Larsson J, de Bruijn MFTR, Martin-Gonzalez J, Pereira CF
  TITLE     GATA2 mitotic bookmarking is required for definitive haematopoiesis.
  JOURNAL   Nat Commun. 2023 Aug 14;14(1):4645. doi: 10.1038/s41467-023-40391-x.
  PUBMED    37580379
REFERENCE   2  (bases 1 to 10547)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-14"; volume: "14(1):4645. doi"; pages: " 
            10.1038/s41467-023-40391-x"
COMMENT     Sequence Label: pFUW-tetO-mCh-Gata2
FEATURES             Location/Qualifiers
     source          1..10547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        408..606
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             624..804
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    851..976
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1469..1702
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1887..1931
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2229..2346
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2403..2421,2422..2444,2445..2463,2464..2486,2487..2505,
                     2506..2528,2529..2547,2548..2570,2571..2589,2590..2612,
                     2613..2631,2632..2654,2655..2673)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2706..2744
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2741..2765
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2856..3557
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     primer_bind     complement(2989..3007)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3257..3276
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3451..3474
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    5028..5616
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5081..5101)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5499..5510)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5619..5635)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5620..5636)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6141..6321
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6347..6364)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6353..6577
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6623..7051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6710..6729)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6920..6941
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7060..7080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7065..7394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7245..7380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7307..7326
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7442..7489
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7508..7882
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8012..8133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8049..8068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8103..8122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8182..8198)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8182..8198)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8195..8217)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8206..8222
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8230..8236,8237..8254,8255..8260))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8275..8296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8413..8430)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8584..9172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8664..8683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9343..10134,10135..10203))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9966..9985
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10204..10308)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10383..10402)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc
       61 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      121 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      181 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      241 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      301 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      361 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
      421 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
      481 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
      541 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
      601 aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
      661 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
      721 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
      781 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
      841 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
      901 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
      961 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     1021 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     1081 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     1141 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     1201 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     1261 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     1321 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     1381 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     1441 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     1501 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     1561 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     1621 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     1681 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     1741 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     1801 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     1861 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     1921 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     1981 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     2041 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     2101 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     2161 ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc
     2221 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     2281 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2341 aattttcggg tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc
     2401 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2461 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2521 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2581 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2701 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2761 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2821 ccagcctccg cggccccgaa ttcaccggtt ctagaatggt gagcaagggc gaggaggtca
     2881 tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg
     2941 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     3001 tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg
     3061 gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc
     3121 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga
     3181 cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca
     3241 acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg
     3301 agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga
     3361 aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc
     3421 aactgcccgg ctactactac gtggacacca agctggacat cacctcccac aacgaggact
     3481 acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca
     3541 tggacgagct gtacaagttc gaagaggtgg cgcctgagca gcctcgctgg atggcgcacc
     3601 ccgccgtatt gaatgcgcag caccccgact cgcaccatcc gggcctggcg cataactaca
     3661 tggagccagc acagctgctg cctcccgacg aggtggatgt cttcttcaac catctcgact
     3721 cgcagggcaa cccttactac gccaacccgg cccacgcgcg cgcgcgcgtt tcctacagcc
     3781 cggcgcatgc ccgtctcacc ggaggccaga tgtgccgacc acacttgttg cacagcccag
     3841 gcttgccgtg gctggacggg ggcaaagcag ctctctctgc cgccgctgcc catcaccaca
     3901 gtccctggac cgtcagcccg ttctccaaga ccccgctgca cccctcagct gctggagcac
     3961 ccggagggcc tctgtctgtt tacccagggg ctgcgggtgg gagcggggga ggcagtggga
     4021 gctccgtggc ctccctcacc cccactgcag cccactcggg ctcccatctc ttcggcttcc
     4081 cacccacgcc acccaaagaa gtgtctccag accccagcac aacaggagct gcttccccgg
     4141 cctcttcttc tgcagggggt agtgtagccc ggggtgagga caaggatggc gtcaagtacc
     4201 aagtgtcact ctccgagagc atgaagatgg aaggcggcag tcccctgcgc ccgggcctag
     4261 ctaccatggg cacccagcct gcaacacacc acccgatacc cacctatccc tcctatgtgc
     4321 ccgccgcagc tcatgactat ggcagcagtc tcttccatcc aggaggcttc ctgggtggcc
     4381 ccgcctccag cttcacccct aagcagagaa gcaaggctcg ctcctgctca gaaggccggg
     4441 agtgtgtcaa ctgtggtgcc acagccaccc ctctctggcg acgagatggc acgggccact
     4501 acctgtgcaa tgcctgtggg ctctaccaca agatgaatgg acagaaccgg ccgctcatca
     4561 agcccaagcg gaggctgtct gctgccagaa gagcgggcac ctgttgtgca aattgtcaga
     4621 cgacaaccac caccttatgg cgccggaacg ccaacgggga ccctgtgtgc aacgcctgtg
     4681 gcctctacta caagctgcac aatgttaaca ggccactgac catgaagaag gaagggatcc
     4741 agacccggaa tcggaagatg tccagcaaat ccaagaaaag caagaaaggg gctgaatgtt
     4801 tcgaggagct ctccaagtgc atgcaagaga agtcaccgcc cttcagtgcg gctgccctgg
     4861 ctggacacat ggcacctgtg ggacacctcc caccttttag tcactctgga cacatcctac
     4921 ccacgcccac gcctatccac ccttcctcca gtctctcttt tggccacccc cacccgtcca
     4981 gcatggtgac tgccatgggc tagttcgaag atatcaagct tatcgataat caacctctgg
     5041 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5101 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5161 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5221 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5281 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5341 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5401 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5461 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5521 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5581 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     5641 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     5701 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     5761 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5821 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5881 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5941 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6001 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6061 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6121 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6181 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6241 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6301 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6361 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6421 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6481 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6541 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6601 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6661 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6721 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6781 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6841 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6901 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6961 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7021 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7141 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7201 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7261 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7321 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7381 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7441 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7501 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7561 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7621 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7681 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7741 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7801 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7861 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7921 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7981 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8041 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8101 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8161 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8221 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8281 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8341 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8401 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8461 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8521 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8581 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8641 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8701 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8761 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8821 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8881 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8941 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9001 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9061 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9121 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9181 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9241 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9301 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9361 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9421 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9481 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9541 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9601 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9661 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9721 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9781 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9841 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9901 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9961 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10021 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10081 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10141 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10201 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10261 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10321 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    10381 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    10441 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
    10501 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcg
//
