LOCUS       sequence-382512-       10018 bp    DNA     circular SYN 15-APR-2025
DEFINITION  C-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG.
            osTIR1 is carried downstream of the C-terminal tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Shively CA, Dong F, Mitra RD
  TITLE     A Suite of New Strain Construction Vectors for Gene Expression 
            Knockdown in Budding Yeast.
  JOURNAL   ACS Synth Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547.
  PUBMED    36650116
REFERENCE   2  (bases 1 to 10018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jan 17. doi: 10.1021/acssynbio.2c00547."
COMMENT     Sequence Label: C-SWAT Compatible pAIDA2(6FLAG)
FEATURES             Location/Qualifiers
     source          1..10018
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             511..1845
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFELSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     CDS             2058..2135
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2163..2183
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2214..2387
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2388..2561
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             2799..2822
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2829..2852
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2859..2882
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2889..2912
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2919..2942
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2949..2972
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      3033..3282
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3049..3067)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(3333..3352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3334..3352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3359..3376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3359..3375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3368..3390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3516..3971
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3603..3622)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3813..3834
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4311..4415
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4416..4484,4485..5276)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4634..4653)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5447..6035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5936..5955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6189..6206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6323..6344
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6359..6364,6365..6382,6383..6389)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6397..6413
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6402..6424
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6421..6437
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6421..6437
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        6458..6476
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6822..7224
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             7243..8967
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             8995..9024
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9031..9060
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9067..9096
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      9152..9349
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     misc_feature    join(9518..10018,1..3)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 atccgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
       61 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
      121 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
      181 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
      241 actgagagtg caccacgctt ttcaattcaa ttcatcacca tcctcatgaa aactgtgtaa
      301 cataataacc gaagtgtcga aaaggtggca ccttgtccaa ttgaacacgc tcgatgaaaa
      361 aaataagata tatataaggt taagtaaagc gtctgttaga aaggaagttt ttcctttttc
      421 ttgctctctt gtcttttcat ctactatttc cttcgtgtaa tacagggtcg tcagatacat
      481 agatacaatt ctattacccc catccataca atgccatctc atttcgatac tgttcaacta
      541 cacgccggcc aagagaaccc tggtgacaat gctcacagat ccagagctgt accaatttac
      601 gccaccactt cttatgtttt cgaaaactct aagcatggtt cgcaattgtt tggtctagaa
      661 gttccaggtt acgtctattc ccgtttccaa aacccaacca gtaatgtttt ggaagaaaga
      721 attgctgcct tagaaggtgg tgctgctgct ttggctgttt cctccggtca agccgctcaa
      781 acccttgcca tccaaggttt ggcacacact ggtgacaaca tcgtttccac ttcttactta
      841 tacggtggta cttataacca gttcaaaatc tcgttcaaaa gatttggtat cgaggctaga
      901 tttgttgaag gtgacaatcc agaagaattc gaaaaggtct ttgatgaaag aaccaaggct
      961 gtttatttgg aaaccattgg taatccaaag tacaatgttc cggattttga aaaaattgtt
     1021 gcaattgctc acaaacacgg tattccagtt gtcgttgaca acacatttgg tgccggtggt
     1081 tacttctgtc agccaattaa atacggtgct gatattgtaa cacattctgc taccaaatgg
     1141 attggtggtc atggtactac tatcggtggt attattgttg actctggtaa gttcccatgg
     1201 aaggactacc cagaaaagtt ccctcaattc tctcaacctg ccgaaggata tcacggtact
     1261 atctacaatg aagcctacgg taacttggca tacatcgttc atgttagaac tgaactatta
     1321 agagatttgg gtccattgat gaacccattt gcctctttct tgctactaca aggtgttgaa
     1381 acattatctt tgagagctga aagacacggt gaaaatgcat tgaagttagc caaatggtta
     1441 gaacaatccc catacgtatc ttgggtttca taccctggtt tagcatctca ttctcatcat
     1501 gaaaatgcta agaagtatct atctaacggt ttcggtggtg tcttatcttt cggtgtaaaa
     1561 gacttaccaa atgccgacaa ggaaactgac ccattcgaac tttctggtgc tcaagttgtt
     1621 gacaatttaa agcttgcctc taacttggcc aatgttggtg atgccaagac cttagtcatt
     1681 gctccatact tcactaccca caaacaatta aatgacaaag aaaagttggc atctggtgtt
     1741 accaaggact taattcgtgt ctctgttggt atcgaattta ttgatgacat tattgcagac
     1801 ttccagcaat cttttgaaac tgttttcgct ggccaaaaac catgagtgtg cgtgatgagt
     1861 tgtaaaatta tgtataaacc tactttctct cacaagtact gcggtgtgaa ataccgcaca
     1921 gatgcgtaag gagaaaatac cgcatcaggc tatattaccc tgttatccct agcgtaactc
     1981 gtacgctgca ggtcgacggt ggcggttctg gcggtggcgg atccggtcga cggatccccg
     2041 ggttaattaa tccatggaag agaagatgga aaaagaattt catagccgtc tcagcagcca
     2101 accgctttaa gaaaatctca tcctccgggg cacttgatta tgatattcca actactgcta
     2161 gcgagaattt gtattttcag ggagaattcg gccttgcgca acacgatgaa gccgtggaca
     2221 acaaattcaa caaagaacaa caaaacgcgt tctatgagat cttacattta cctaacttaa
     2281 acgaagaaca acgaaacgcc ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta
     2341 accttttagc agaagctaaa aagctaaatg atgctcaggc gccgaaagta gacaacaaat
     2401 tcaacaaaga acaacaaaac gcgttctatg agatcttaca tttacctaac ttaaacgaag
     2461 aacaacgaaa cgccttcatc caaagtttaa aagatgaccc aagccaaagc gctaaccttt
     2521 tagcagaagc taaaaagcta aatgatgctc aggcgccgaa agtagacgcg aatcatcagc
     2581 gtacgggtgc aggcgccggg gctggtgctg gggcaggtac acctaaagat ccagccaaac
     2641 ctccggccaa ggcacaagtt gtgggatggc caccggtgag atcataccgg aagaacgtga
     2701 tggtttcctg ccaaaaatca agcggtggcc cggaggcggc ggcgttcgtg aaggtatcac
     2761 ccgggataca ttatacgaag ttatgcatgc tcactagtga ctacaaggac gacgatgaca
     2821 agtctagtga ctacaaggac gacgatgaca agtctagtga ctacaaggac gacgatgaca
     2881 agtctagtga ctacaaggac gacgatgaca agtctagtga ctacaaggac gacgatgaca
     2941 agtctagtga ctacaaggac gacgatgaca agtctagatg aggcgcgcta gatctgttta
     3001 gcttaagaca ggcccctttt cctttgtcga tatcatgtaa ttagttatgt cacgcttaca
     3061 ttcacgccct cctcccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt
     3121 ctaggtccct atttattttt tttaatagtt atgttagtat taagaacgtt atttatattt
     3181 caaatttttc ttttttttct gtacaaacgc gtgtacgcat gtaacattat actgaaaacc
     3241 ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctggc gcgccagatc
     3301 tgcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat tacaattcac
     3361 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     3421 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     3481 cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag
     3541 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3601 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3661 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3721 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3781 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3841 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3901 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     3961 gcttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     4021 attgaaaagc gtggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
     4081 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4141 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     4201 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     4261 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4321 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4381 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4441 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4501 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4561 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4621 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     4681 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     4741 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     4801 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     4861 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     4921 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     4981 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5041 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5101 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5161 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5221 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5281 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5341 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5401 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5461 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5521 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5581 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     5641 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     5701 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     5761 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     5821 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     5881 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     5941 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6001 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6061 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6121 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6181 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6241 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6301 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6361 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6421 caggaaacag ctatgaccat gattacgcca agctcgaaat taaccctcac taaagggaac
     6481 aaaagctggt accgggcccc ccctcgacgg tatcgataag cttgatatcg aattcgggtg
     6541 tacaatatgg acttcctctt ttctggcaac caaacccata catcgggatt cctataatac
     6601 cttcgttggt ctccctaaca tgtaggtggc ggaggggaga tatacaatag aacagatacc
     6661 agacaagaca taatgggcta aacaagacta caccaattac actgcctcat tgatggtggt
     6721 acataacgaa ctaatactgt agccctagac ttgatagcca tcatcatatc gaagtttcac
     6781 tacccttttt ccatttgcca tctattgaag taataatagg cgcatgcaac ttcttttctt
     6841 ttttttttct tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaat
     6901 gatggaagac actaaaggaa aaaattaacg acaaagacag caccaacaga tgtcgttgtt
     6961 ccagagctga tgaggggtat ctcgaagcac acgaaacttt ttccttcctt cattcacgca
     7021 cactactctc taatgagcaa cggtatacgg ccttccttcc agttacttga atttgaaata
     7081 aaaaaaagtt tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt
     7141 ttcctcgtca ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa
     7201 gctataccaa gcatacaatc aactatctca tatacaacta gaatgacgta cttcccggag
     7261 gaggtggtgg agcacatctt cagcttcctg ccggcgcagc gcgaccgcaa cacggtctcg
     7321 ctcgtctgca aggtgtggta cgagatcgag aggctgagcc gccgcggcgt cttcgtgggc
     7381 aactgctacg ccgtgcgcgc cggccgcgtc gccgcgcggt tccccaacgt gcgggcgctc
     7441 acggtgaagg ggaagcccca cttcgccgac ttcaacctcg tgccccccga ctggggcggc
     7501 tacgcggggc cgtggatcga ggcggccgcg aggggatgcc acggcctgga ggagctcagg
     7561 atgaagcgga tggtggtgtc cgacgagagc ctcgagctgc tggctcgctc gttcccgcgg
     7621 ttcagggctc ttgttcttat cagctgcgag gggttcagca ctgacgggct agccgccgtc
     7681 gcgagccatt gcaagcttct gagggagttg gatttgcagg aaaatgaagt ggaggatcga
     7741 gggcctaggt ggctttcctg cttccctgat tcctgcacat cacttgtctc attgaatttt
     7801 gcctgcatca aaggggaggt taatgctggt tcactggaga gacttgttag caggtcccca
     7861 aacctgcgga gtttgaggct gaatcgatct gtatcggtag atacacttgc aaagatacta
     7921 ctgcgtaccc ctaacttgga ggatttgggg acagggaatt tgacagatga cttccaaact
     7981 gagtcctact ttaagcttac cagtgctctg gagaaatgca agatgttgag gagtttgtct
     8041 ggattctggg atgcttctcc tgtttgcctg tcatttatct accccctgtg tgctcaactg
     8101 acaggattga acttgagcta tgcacccaca cttgatgctt ctgaccttac aaaaatgatt
     8161 agccgctgtg tgaagctcca acgcctttgg gtactggatt gtatctcgga caaaggcttg
     8221 caagtggtgg cctccagttg caaagacttg caagaactca gggtatttcc atcagatttc
     8281 tacgtagctg gttattctgc agtgacagag gagggacttg ttgcagtatc cttgggctgt
     8341 ccaaaactga actcactact gtacttctgt caccaaatga ctaatgctgc actagttact
     8401 gtcgccaaga actgtccaaa tttcacacga ttcagacttt gtattcttga gccagggaag
     8461 cctgatgttg tgacaagcca accattagat gaaggctttg gagctattgt tcgtgagtgc
     8521 aagggattac aacgtttgtc aatatctggt cttctcacag acaaagtttt catgtatatt
     8581 gggaaatatg caaaacaact tgagatgctt tctatagcat ttgctggtga cagtgataag
     8641 ggtatgatgc atgttatgaa tggatgcaag aatttaagga aactggagat aagagatagc
     8701 ccgtttggtg atgctgcact cttggggaat tttgctaggt acgagacaat gcgatccctt
     8761 tggatgtcat cttgcaatgt cacgttaaag gggtgccaag tccttgcgtc aaagatgccg
     8821 atgctcaatg ttgaggtcat aaatgagcgg gatggtagca atgaaatgga ggaaaaccat
     8881 ggagatctgc ctaaagtgga gaaattatat gtgtaccgca caactgctgg ggcgagggat
     8941 gatgcaccaa attttgttaa aatcctagtc gactccggtt ctgctgctag tggtgaacaa
     9001 aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga ggaagacttg
     9061 aacggtgaac aaaaattaat ctcagaagaa gacttgaacg gatccactag ctaaaaagtg
     9121 gaacgatcat tcaagagatc cccgggagag tactgacaat aaaaagattc ttgttttcaa
     9181 gaacttgtca tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag
     9241 cgtgatttat attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca
     9301 gaaagtaata tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg
     9361 atactaacgc cgccatccag tttaaacgag ctccaattga gttcttcttt gagatatcga
     9421 ttgaacgagc tcgaattcat cgatctatat taccctgtta tccctagcgt aactttccgc
     9481 gcacatttcc ccgaaaagtg ccacctgggt ccttttcatc acgtgctata aaaataatta
     9541 taatttaaat tttttaatat aaatatataa attaaaaata gaaagtaaaa aaagaaatta
     9601 aagaaaaaat agtttttgtt ttccgaagat gtaaaagact ctagggggat cgccaacaaa
     9661 tactaccttt tatcttgctc ttcctgctct caggtattaa tgccgaattg tttcatcttg
     9721 tctgtgtaga agaccacaca cgaaaatcct gtgattttac attttactta tcgttaatcg
     9781 aatgtatatc tatttaatct gcttttcttg tctaataaat atatatgtaa agtacgcttt
     9841 ttgttgaaat tttttaaacc tttgtttatt tttttttctt cattccgtaa ctcttctacc
     9901 ttctttattt actttctaaa atccaaatac aaaacataaa aataaataaa cacagagtaa
     9961 attcccaaat tattccatca ttaaaagata cgaggcgcgt gtaagttaca ggcaagcg
//
