LOCUS       sequence-382600-        9116 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Empty Vector control modified to express GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9116)
  AUTHORS   Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, 
            Perrimon N, Signoretti S, Kaelin WG Jr
  TITLE     HIF-independent synthetic lethality between CDK4/6 inhibition and 
            VHL loss across species.
  JOURNAL   Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 
            10.1126/scisignal.aay0482.
  PUBMED    31575731
REFERENCE   2  (bases 1 to 9116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2019-10-1"; volume: "12(601). pii: 12/601/eaay0482.
            doi"; pages: " 10.1126/scisignal.aay0482"
COMMENT     Sequence Label: pLX304-EV-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        66..369
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        370..573
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     524..544
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    596..620
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(686..710)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             712..753
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    join(795..1369,1370..1372,1373..1381)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(962..979)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1189..1208
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1382..1384,1385..1387,1388..2101)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1382..1384,1385..1387,1388..2098)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1427..1448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1688..1707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2035..2056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2124..2712
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2177..2197)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2595..2606)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2784..3017
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3095..3216
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3132..3151)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3186..3205
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3256..3391
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3411..3430)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3412..3430)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3440..3457)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3440..3456)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3449..3471)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3598..4053
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3685..3704)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3895..3916
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4079..4183
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4184..4252,4253..5044)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4402..4421)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5215..5803
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5704..5723
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5957..5974
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6091..6112
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6127..6132,6133..6150,6151..6157)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6165..6181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6170..6192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6189..6205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6189..6205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6224..6244
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6226..6244
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6272..6498
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6499..6679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6726..6851
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7344..7577
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7762..7806
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7981..8481
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8400..8418
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8493..8888
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8948..9065
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      541 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag
      601 tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccgaat
      661 tcgcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt tggtaagcct
      721 atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagccg ctacgtaaat
      781 tccgcccccc cccccccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
      841 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
      901 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
      961 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     1021 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     1081 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     1141 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     1201 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     1261 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     1321 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     1381 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1441 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1501 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1561 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1621 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1681 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1741 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1801 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1861 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1921 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     1981 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2041 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2101 aaccggtggc gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
     2161 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     2221 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     2281 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     2341 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     2401 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     2461 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     2521 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     2581 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     2641 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     2701 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
     2761 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     2821 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     2881 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     2941 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     3001 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
     3061 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
     3121 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     3181 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
     3241 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     3301 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     3361 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
     3421 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     3481 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     3541 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
     3601 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     3661 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     3721 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     3781 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     3841 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     3901 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     3961 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     4021 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     4081 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4141 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4201 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4261 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4321 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4381 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4441 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4501 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4561 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4621 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4681 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     4741 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     4801 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     4861 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     4921 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     4981 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5041 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5101 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5161 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5221 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5281 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5341 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5401 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5461 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5521 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5581 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5641 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     5701 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     5761 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     5821 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     5881 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     5941 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6001 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     6061 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     6121 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     6181 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     6241 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
     6301 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
     6361 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
     6421 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
     6481 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     6541 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     6601 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     6661 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
     6721 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
     6781 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
     6841 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
     6901 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
     6961 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
     7021 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
     7081 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
     7141 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     7201 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     7261 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     7321 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     7381 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     7441 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     7501 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     7561 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     7621 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     7681 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     7741 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     7801 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     7861 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     7921 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     7981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     8041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     8101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     8161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     8221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     8281 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     8341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     8401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     8461 atcaccgacc tctctcccca gggggtacca ccatggccaa gcctttgtct caagaagaat
     8521 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca
     8581 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     8641 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     8701 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     8761 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     8821 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     8881 gggagggcct gcagctgcag tagtaagaat tctagatctt gagacaaatg gcagtattca
     8941 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     9001 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     9061 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gagggg
//
