LOCUS       sequence-384058-       10143 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Sars-CoV-2 S_A701V in MAC-C destination vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Varjosalo M, Huuskonen S, Liu X, Redchuk T, Salokas K, Lundberg R, 
            Maljanen S, Belik M, Reinholm A, Kolehmainen P, Tripathi G, Laine P,
            Belanov S, Auvinen P, Vartiainen M, Laine L, Osterlund P, Julkkunen 
            I, Kakkola L
  TITLE     The comprehensive SARS-CoV-2 'hijackome' knowledge base— reveals 
            significant changes in host cell protein expression and activation 
            by multiple SARS-CoV-2 variants
  JOURNAL   10 May 2024, Research Square
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "10 May 
            2024, Research Square"
COMMENT     Sequence Label: MAC-C Sars-CoV-2 S_A701V
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(528..1319,1320..1388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1151..1170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1389..1493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1568..1587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1759..2138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2139..2342
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2293..2313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2344..2362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2365..2383
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2393..2417
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2560..2584
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6404..6428)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6475..6498
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6535..6558
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6589..6615
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6589..6615
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(6616..6963,6964..6966,6967..7575)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     primer_bind     complement(7619..7636)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7625..7849
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7982..8001)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    8133..8180
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             8188..9210
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             8188..9210
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    9340..9461
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9377..9396)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9431..9450
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9510..9526)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9510..9526)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9523..9545)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9534..9550
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9558..9564,9565..9582,9583..9588))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9603..9624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9741..9758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9912..10143,1..357))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9992..10011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
       61 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
      421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
      481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      661 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
      721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     1381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1501 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     1561 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     1621 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     1681 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     1741 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     1801 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     1861 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     1921 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     1981 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     2041 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     2101 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     2161 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     2221 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     2281 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     2341 ctctccctat cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt
     2401 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
     2461 accgggaccg atccagcctc cggactctag cgtttaaact taagcttggt accgagctcg
     2521 gatccactag tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagtt
     2581 ggcatgttcg tgttcctggt gctgctgcca ctggtgagct cccagtgcgt gaacctgacc
     2641 accagaaccc agctgccacc tgcctacacc aactccttca ccaggggcgt gtactaccca
     2701 gacaaggtgt tcagatccag cgtgctgcac tctacccagg acctgttcct gccattcttc
     2761 tctaacgtga cctggttcca cgccatccac gtgtctggaa ccaacggcac caagagattc
     2821 gacaacccag tgctgccatt caacgacggc gtgtacttcg cttctaccga gaagtctaac
     2881 atcatcaggg gttggatctt cggaaccacc ctggactcta agacccagtc tctgctgatc
     2941 gtgaacaacg ctaccaacgt ggtgatcaag gtgtgcgagt tccagttctg caacgaccca
     3001 ttcctgggag tgtactacca caagaacaac aagtcttgga tggaaagcga gttcagggtg
     3061 tacagctctg ctaacaactg caccttcgag tacgtgtctc agcctttcct gatggacctg
     3121 gaaggaaagc agggaaactt caagaacctg agagagttcg tgttcaagaa catcgacggt
     3181 tacttcaaga tatacagcaa gcacacccca atcaacctgg tgagggacct gccacagggt
     3241 ttctctgctc tggagcccct ggtggacctg ccaatcggca tcaacatcac caggttccag
     3301 accctgctgg ctctgcacag gtcttacctg accccaggcg acagcagctc tggatggacc
     3361 gccggcgccg ctgcttacta cgtgggatac ctgcagccaa ggaccttcct gctgaagtac
     3421 aacgaaaacg gcaccatcac cgacgccgtg gactgcgctc tggacccact gtctgagacc
     3481 aagtgcaccc tgaagtcttt caccgtggaa aagggaatct accagaccag caacttccgc
     3541 gtgcagccca ccgagtctat cgtgagattc ccaaacatca ccaacctgtg cccattcgga
     3601 gaagtgttca acgctaccag gttcgccagc gtgtacgctt ggaacaggaa gagaatcagc
     3661 aactgcgtgg ccgactactc tgtgctgtac aactctgcct ccttctccac cttcaagtgc
     3721 tacggcgtgt ccccaaccaa gctgaacgac ctgtgcttca ccaacgtgta cgctgactcc
     3781 ttcgtgatca gaggagacga agtgagacag atcgctcccg gtcagaccgg aaagatcgcc
     3841 gactacaact acaagctgcc agacgacttc accggttgcg tgatcgcttg gaactctaac
     3901 aacctggact ctaaggtggg aggcaactac aactacctgt acaggctgtt ccgcaagagc
     3961 aacctgaagc ctttcgaaag ggacatcagc accgaaatct accaggctgg atctacccca
     4021 tgcaacggag tggaaggatt caactgctac ttccctctgc agtcttacgg attccagcca
     4081 accaacggag tgggatacca gccatacaga gtggtggtgc tgtctttcga gctgctgcac
     4141 gctcctgcta ccgtgtgcgg accaaagaag tctaccaacc tggtgaagaa caagtgcgtg
     4201 aacttcaact tcaacggact gaccggaacc ggagtgctga ccgaatccaa caagaagttc
     4261 ctgcctttcc agcagttcgg tagagacatc gccgacacca ccgacgctgt gagagaccca
     4321 cagaccctgg aaatcctgga catcaccccc tgctctttcg gaggagtgag cgtgatcacc
     4381 ccaggaacca acacctccaa ccaggtggcc gtgctgtacc aggacgtgaa ctgcaccgaa
     4441 gtgccagtgg ccatccacgc tgaccagctg accccaacct ggagagtgta cagcaccgga
     4501 tctaacgtgt tccagacccg tgccggctgc ctgatcggag ccgagcacgt gaacaactcc
     4561 tacgaatgcg acatcccaat cggagctggc atctgcgctt cttaccagac ccagaccaac
     4621 agcccaagaa gggccagatc cgtggcctct cagagcatca tcgcctacac catgagcctg
     4681 ggcgttgaaa acagcgtggc ttacagcaac aactctatcg ccatcccaac caacttcacc
     4741 atctctgtga ccaccgaaat cctgccagtg agcatgacca agaccagcgt ggactgcacc
     4801 atgtacatct gcggagactc taccgaatgc tctaacctgc tgctgcagta cggcagcttc
     4861 tgcacccagc tgaaccgcgc tctgaccgga atcgctgtgg agcaggacaa gaacacccag
     4921 gaagtgttcg ctcaggtgaa gcagatatac aagaccccac ctatcaagga cttcggagga
     4981 ttcaacttct ctcagatcct gcccgaccca tctaagccct ctaagaggtc tttcatcgaa
     5041 gacctgctgt tcaacaaggt gaccctggcc gacgccggtt tcatcaagca gtacggtgac
     5101 tgcctgggag acatcgccgc tagagacctg atctgcgccc agaagttcaa cggtctgacc
     5161 gtgctgccac cactgctgac cgacgagatg atcgcccagt acacctctgc cctgctggct
     5221 ggaaccatca cctctggttg gaccttcgga gctggcgccg ccctgcagat cccattcgct
     5281 atgcagatgg cctaccgttt caacggtatc ggagtgaccc agaacgtgct gtacgaaaac
     5341 cagaagctga tcgctaacca gttcaactct gctatcggaa agatccagga ctccctgtcc
     5401 tctaccgctt ctgccctggg aaagctgcag gacgtggtga accagaacgc ccaggccctg
     5461 aacaccctgg tgaagcagct gtcttctaac ttcggagcca tctcctctgt gctgaacgac
     5521 atcctgtcta ggctggacaa ggtggaagcc gaggtgcaga tcgacagact gatcaccgga
     5581 agactgcagt ctctgcagac ctacgtgacc cagcagctga tcagggccgc tgaaatcaga
     5641 gcttctgcca acctggctgc caccaagatg tccgaatgcg tgctgggcca gtctaagagg
     5701 gtggacttct gcggaaaggg ataccacctg atgtccttcc cacagtctgc tccacacgga
     5761 gtggtgttcc tgcacgtgac ctacgtgccc gcccaggaaa agaacttcac caccgcccca
     5821 gctatctgcc acgacggaaa ggctcacttc cccagggagg gagtgttcgt gtctaacgga
     5881 acccactggt tcgtgaccca gaggaacttc tacgagccac agatcatcac caccgacaac
     5941 accttcgtgt ctggaaactg cgacgtggtg atcggaatcg tgaacaacac cgtgtacgac
     6001 ccactgcagc cagaactgga ctctttcaag gaggaactgg acaagtactt caagaaccac
     6061 acctctccag acgtggacct gggagacatc tccggaatca acgcctctgt ggtgaacatc
     6121 cagaaggaaa tcgacagact gaacgaggtg gccaagaacc tgaacgaatc tctgatcgac
     6181 ctgcaggaac tgggaaagta cgagcagtac atcaagtggc cctggtacat ctggctgggt
     6241 ttcatcgctg gtctgatcgc tatcgtgatg gtgaccatca tgctgtgctg catgacctct
     6301 tgctgctctt gcctgaaggg atgctgctct tgcggatctt gctgcaagtt cgacgaggac
     6361 gactccgagc ctgtgctgaa gggtgtgaag ctgcactaca cctacccaac tttcttgtac
     6421 aaagtggttg atatcaaggc cgccgacatc accagcctgt acaagaaagt ggggtggagc
     6481 catccccagt tcgagaaggg cggaggatct ggcggcggaa gcggcggagg aagctggtcc
     6541 caccctcagt ttgagaaagg caccgagctg ggcagcacca tggccagcta cccctacgac
     6601 gtgcccgact acgccaagga caacaccgtg cccctgaagc tgatcgccct gctggccaac
     6661 ggcgagttcc actctggcga gcagctgggc gagacactgg gcatgagcag agccgccatc
     6721 aacaagcaca tccagaccct gcgggactgg ggcgtggacg tgttcacagt gcccggcaag
     6781 ggctacagcc tgcccgagcc catccagctg ctgaacgcca agcagatcct gggccagctg
     6841 gatggcggat ctgtggccgt gctgcccgtg atcgacagca ccaaccagta tctgctcgac
     6901 cggatcggcg agctgaagtc cggcgacgcc tgtatcgccg agtaccagca ggccggcaga
     6961 ggcggcaggg gcagaaagtg gttcagcccc ttcggcgcca acctgtacct gagcatgttc
     7021 tggagactgg aacagggccc agccgccgct attggcctga gcctggtcat cggcatcgtg
     7081 atggccgagg tgctgcggaa gctgggcgcc gacaaagtgc gcgtgaagtg gcccaacgac
     7141 ctgtatctgc aggaccggaa gctggccggc atcctggtgg aactgaccgg caaaacaggc
     7201 gacgccgccc agatcgtgat cggcgctggc atcaacatgg ccatgcggcg ggtggaagag
     7261 agcgtggtca accagggctg gatcaccctg caggaagccg gcatcaacct ggaccggaac
     7321 accctggccg ccatgctgat cagagagctg agagccgccc tggaactgtt cgagcaggaa
     7381 ggcctggccc cctacctgag cagatgggag aagctggaca acttcatcaa ccggcccgtg
     7441 aaactgatca tcggcgacaa agagatcttc ggcatcagcc ggggcatcga caagcagggg
     7501 gccctgctcc tggaacagga cggcatcatc aagccctgga tgggcggcga gatcagcctg
     7561 cggagcgccg agaagtgatg actcgagtct agagggcccg tttaaacccg ctgatcagcc
     7621 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7681 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7741 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7801 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7861 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     7921 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7981 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8041 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8101 aaacttgatt agggtgatgg ttcacgtacc tagaagttcc tattccgaag ttcctattct
     8161 ctagaaagta taggaacttc cttggccaaa aagcctgaac tcaccgcgac gtctgtcgag
     8221 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     8281 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     8341 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     8401 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     8461 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     8521 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     8581 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     8641 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     8701 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     8761 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     8821 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     8881 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     8941 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     9001 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     9061 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     9121 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     9181 cccagcactc gtccgagggc aaaggaatag cacgtactac gagatttcga ttccaccgcc
     9241 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     9301 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     9361 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9421 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     9481 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9541 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9601 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9661 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     9721 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     9781 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     9841 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     9901 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9961 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10021 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10081 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    10141 gtc
//
