LOCUS       sequence-384142-       11989 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Drosophila QF:Gal4 expression under introduced sequence control, 
            PhiC31 transgenesis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11989)
  TITLE     McCabe lab pBID2 plasmids 2021
REFERENCE   2  (bases 1 to 11989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBID2_MCS>QF:Gal4
FEATURES             Location/Qualifiers
     source          1..11989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..76
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     68..85
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     69..85
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     92..111
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        92..110
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(144..162)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(614..685,1087..1360,1435..2089,2151..2466,2670..2801,
                     2872..3486)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4261..4294
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4307..4737
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     intron          4973..5039
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     protein_bind    8518..8948
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    8982..9051
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9270..9288)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9309..9325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9309..9325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9322..9344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9333..9349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9357..9363,9364..9381,9382..9387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9402..9423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9540..9557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9711..10299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9791..9810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10470..11261,11262..11330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11093..11112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11331..11435)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11461..11916)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11598..11619)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11810..11829
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
       61 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattggg
      121 tacgtaccgg gcccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga
      181 taaaaaaaac attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt
      241 taaatttact tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc
      301 aaaatgacat tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc
      361 gccgtctctt tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag
      421 cgtcaatgtc cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt
      481 ttacgccatc aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt
      541 ttgttttttg agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg
      601 aaaaatcccg gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca
      661 cccatctgcc gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact
      721 tgatctatag gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca
      781 caaaaatccc aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta
      841 gctgttctcg ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc
      901 attcattttc gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga
      961 tcctagatgc acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta
     1021 ctttttgttc ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca
     1081 ttgcagggtg acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc
     1141 aaaaactacg gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc
     1201 caactagccg agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat
     1261 cagccgggct ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag
     1321 cgacacatac cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc
     1381 ggctgatctg tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg
     1441 gcgtggccta tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga
     1501 ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga
     1561 tgcgactgct caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg
     1621 tccagcagga tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag
     1681 ccatggtgcg gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg
     1741 tgatccagga gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg
     1801 tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg
     1861 atccgccgct tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca
     1921 gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc
     1981 atcagccgtc ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca
     2041 gggtagcttt cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat
     2101 gtgtttatta agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc
     2161 cagtgtccta ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc
     2221 ggacgggaga tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc
     2281 aaagtagccc gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag
     2341 cagccggaga atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg
     2401 tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag
     2461 acaacggtga gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa
     2521 attcgctaga ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga
     2581 gatgtatagt ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa
     2641 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
     2701 tgggccaaca actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc
     2761 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata
     2821 catttgcatt tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
     2881 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     2941 tttctgggca aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg
     3001 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     3061 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     3121 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     3181 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     3241 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     3301 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     3361 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
     3421 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     3481 gagtagccga catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc
     3541 gtgtttactg tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa
     3601 caagatatga cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt
     3661 ggatttgtct tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt
     3721 atcgctacat aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc
     3781 tcttcataat tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc
     3841 cgctctccgc aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc
     3901 gcgcgattcg gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt
     3961 aataaatatt tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca
     4021 gctatattta ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg
     4081 gagtgattag cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga
     4141 tgcatctcaa aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact
     4201 ataataataa gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctagc
     4261 ataacttcgt ataatgtatg ctatacgaag ttatgctagc ggatcctggc cacgtaataa
     4321 gtgtgcgttg aatttattcg caaaaacatt gcatattttc ggcaaagtaa aattttgttg
     4381 cataccttat caaaaaataa gtgctgcata ctttttagag aaaccaaata attttttatt
     4441 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
     4501 tttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacccgtt
     4561 tttaataaaa tacattgcat accctctttt aataaaaaat attgcatacg ttgacgaaac
     4621 aaattttcgt tgcataccca ataaaagatt attatattgc ataccttttc ttgccatacc
     4681 atttagccga tcaattgtgc tcggcaacag tatatttgtg gtgtgccaac caacaacaga
     4741 tctgaattcg cttaattaag gcgcgccgca catttaaatc tcgaggataa acggccggcc
     4801 gagctcgccc ggggatcgag cgcagcggta taaaagggcg cggggtggct gagagcatca
     4861 gttgtgaatg aatgttcgag ccgagcagac gtgccgctgc cttcgttaat atcctttgaa
     4921 taagccaact ttgaatcaca agacgcatac caaacggtac cagatctaaa aggtaggttc
     4981 aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct ttacttcagg
     5041 cggccgcgtt taaacaactt aaaaaaaaaa atcaaaatgc cacccaagcg caaaacgctt
     5101 aacgctgcgg ctgaggctaa cgctcatgcc gacggacacg ccgacggaaa cgccgacgga
     5161 cacgtggcca atacggccgc gtcctcgaat aatgcgaggt tcgctgatct cactaacatc
     5221 gatactccgg gtctgggacc cacaactacg accctgctcg tggaaccagc acgctcaaag
     5281 cgtcaacgag tgtcccgcgc atgcgaccag tgccgtgcag cccgagagaa atgcgacgga
     5341 atacagcctg cgtgtttccc gtgcgtttcc cagggaaggt cctgcactta tcaggcttcg
     5401 ccgaaaaaga ggggagttca aaccggttat attcgtacgc tggagctcgc cctcgcctgg
     5461 atgtttgaaa atgtcgcgcg ttccgaagat gccttgcata acctcctcgt ccgtgacgcc
     5521 ggacaaggat cagctctgct cgttggtaaa gattcgccgg ctgccgagcg actccatgcc
     5581 cgttgggcta ctagccgtgt caataagagc attacccgcc tcctcatcga cagtgcggcc
     5641 caccacgaca acagcaccat tccactggac ttcatgcctc gtgacgcact gcatggattt
     5701 gactggagcg aggaggatga tatgagcgac ggactcccat tcctgaagac cgaccccaat
     5761 aacaatggtt tctttggcga cggcagcctg ctgtgtatcc tgcgaagtat tggttttaag
     5821 ccggaaaatt acacgaacag taatgtcaat cgtcttccca caatgatcac cgaccgttac
     5881 acgctggcca gtcgcagtac tacaagccgc ctgctgcaaa gctatcttaa caacttccac
     5941 ccctactgcc ccatagtgca tagtcctact ctcatgatgc tttacaacaa tcagattgag
     6001 atagcatcga aggaccagtg gcagatcctc ttcaactgca ttctcgccat cggcgcttgg
     6061 tgtattgaag gcgagagcac agatatcgac gtgttttact atcaaaacgc caagtcgcac
     6121 ctcacctcga aggtcttcga aagtggaagc attatcctcg tgacagccct ccatctgctg
     6181 agtcgttata cccagtggcg tcagaaaact aatacatcct acaactttca ttcgttcagc
     6241 attcggatgg cgattagcct gggacttaat cgtgacctcc caagtagctt cagcgattcg
     6301 tcgatcctgg aacagcgccg gaggatttgg tggagtgtgt acagctggga gatccagctg
     6361 tccctcttgt acggacgcag cattcagctg agtcagaaca cgatttcctt cccgtccagc
     6421 gtggacgacg ttcaacggac aactacgggc ccgaccatct accacggtat cattgagacg
     6481 gcccgcttgc ttcaagtgtt tacgaagatt tatgagctgg ataagacagt gaccgcggag
     6541 aagagtccga tctgcgccaa gaaatgcctg atgatctgca atgagataga ggaggtgagc
     6601 cgccaggcac ccaagttcct gcagatggac ataagcacta cggcgctgac taatctcctc
     6661 aaagaacacc cctggctgag tttcacccgc ttcgagctga agtggaaaca gctgagtctg
     6721 attatatacg ttttgcggga ctttttcaca aattttacgc agaagaagtc gcagcttgag
     6781 caagatcaga acgaccatca gtcctacgag gtaaagaggt gcagcataat gctctccgac
     6841 gcagcccagc gcaccgtgat gtcggtgtcg tcctatatgg acaaccataa tgtgacccct
     6901 tatttcgcct ggaattgctc ctactatttg ttcaacgccg tgttggtccc aatcaagact
     6961 cttctgagca actccaaatc gaacgccgag aataacgaga cagcacagct cttgcagcaa
     7021 attaacaccg tgttgatgct gttgaagaaa ctcgctacat tcaagattca gacttgcgaa
     7081 aagtacatcc aggtgttgga agaggtgtgc gccccattcc ttctttcgca atgcgcaatt
     7141 cccctcccgc acataagtta caataattcc aacggctccg ctatcaagaa tatcgtcggc
     7201 tccgctacaa tcgcgcagta tcccacgctg cctgaggaaa atgttaacaa catatcggtg
     7261 aagtatgtaa gtccaggcag cgtcggcccg agccctgtgc cgctgaaaag cggagcctcc
     7321 ttcagcgatc tggtcaagct gctcagcaac cgcccaccat cccgcaactc ccctgtcact
     7381 ataccccgat cgaccccgtc gcatcgtagc gttaccccat tcctgggaca acagcagcaa
     7441 ctgcagagtc tggtgccact tacgccctcg gcattgtttg gcggagccaa cttcaaccag
     7501 agtggtaaca ttgcggacag ctccttgagc ttcacgttta ccaacagctc caacggtcca
     7561 aacttgatca cgactcagac aaactcccag gcattgtccc agcccattgc cagctcgaat
     7621 gtgcatgata actttatgaa taatgaaatt actgcctcca agattgacga cggcaacaac
     7681 agcaagcccc tttccccagg ctggactgac cagacggcct acaatgcttt cggtatcacc
     7741 actggcatgt tcaacaccac aacgatggac gacgtatata actatctttt tgatgatgag
     7801 gatactccac cgaacccaaa gaaggagtag cctgcaggaa tgaatcgttt ttaaaataac
     7861 aaatcaattg ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt
     7921 aggaagatta cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac
     7981 gccacaattg tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct
     8041 ttgttcatag acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg
     8101 tatagcgagc tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt
     8161 tcatcaccct cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca
     8221 taatctaata aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc
     8281 gaaaagaact cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca
     8341 aagtacgcag tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg
     8401 ttgtaaccga acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc
     8461 acgatgccga caatggagct ggcttggata gcgattcgag ttaactctag aagatcttgg
     8521 ccacgtaata agtgtgcgtt gaatttattc gcaaaaacat tgcatatttt cggcaaagta
     8581 aaattttgtt gcatacctta tcaaaaaata agtgctgcat actttttaga gaaaccaaat
     8641 aattttttat tgcatacccg tttttaataa aatacattgc ataccctctt ttaataaaaa
     8701 atattgcata ctttgacgaa acaaattttc gttgcatacc caataaaaga ttattatatt
     8761 gcatacccgt ttttaataaa atacattgca taccctcttt taataaaaaa tattgcatac
     8821 gttgacgaaa caaattttcg ttgcataccc aataaaagat tattatattg catacctttt
     8881 cttgccatac catttagccg atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa
     8941 ccaacaacgg atccactagt gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     9001 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     9061 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     9121 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     9181 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     9241 tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc gagcttggcg
     9301 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     9781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     9841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     9901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     9961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    10801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    10861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    10921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    10981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11461 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
    11521 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
    11581 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
    11641 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
    11701 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
    11761 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
    11821 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
    11881 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
    11941 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagct
//
