LOCUS       sequence-384352-       12217 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral Integratable Inducible synZiFTR ZF3 + Reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12217)
  AUTHORS   Li HS, Israni DV, Gagnon KA, Gan KA, Raymond MH, Sander JD, Roybal 
            KT, Joung JK, Wong WW, Khalil AS
  TITLE     Multidimensional control of therapeutic human cell function with 
            synthetic gene circuits.
  JOURNAL   Science. 2022 Dec 16;378(6625):1227-1234. doi: 
            10.1126/science.ade0156. Epub 2022 Dec 15.
  PUBMED    36520914
REFERENCE   2  (bases 1 to 12217)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade0156"; journalName: "Science"; date: 
            "2022-12-16- 16"; volume: "378"; issue: "6625"; pages: "1227-1234"
COMMENT     Sequence Label: pMN-333
FEATURES             Location/Qualifiers
     source          1..12217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1002..1067
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1197..1217
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1642..1776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1679..1698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1733..1752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1944..2577
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2624..2749
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3246..3479
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3664..3708
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3993..4110
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4179..4889)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4457..4476)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4726..4744
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      4886..4895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5436..5843
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6627..6998
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             7023..7958
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             7071..7964
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     misc_feature    8024..8612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8077..8097)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8495..8506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8615..8631)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8616..8632)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8722..8955
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8980..8998)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8981..8998)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9288..9307)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9521..9540)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9640..9662
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9700..9718)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9786..9890
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9891..9959,9960..10751)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10109..10128)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10922..11510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11411..11430
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11664..11681
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11751..11771)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11756..12085
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11936..12071
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac
       61 acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa
      121 actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg
      181 cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt
      241 tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa
      301 ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt
      361 gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt
      421 cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg
      481 tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg
      541 gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat
      601 tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt
      661 taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt
      721 gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat
      781 gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat
      841 gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg
      901 caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga
      961 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     1021 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     1081 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     1141 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     1201 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     1261 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     1321 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     1381 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     1441 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     1501 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     1561 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     1621 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     1681 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     1741 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     1801 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     1861 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     1921 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     1981 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     2041 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     2101 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     2161 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     2221 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     2281 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     2341 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     2401 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2461 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2521 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2581 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2641 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2701 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2761 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2821 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     2881 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     2941 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3001 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3061 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     3121 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3181 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     3241 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     3301 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     3361 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     3421 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     3481 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     3541 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     3601 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     3661 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     3721 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     3781 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     3841 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     3901 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     3961 cggtatcgcc aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4021 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4081 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     4141 gtttggatcg ataagcttga tatcgaattc ctgcagcctt acttgtacag ctcgtccatg
     4201 ccgccggtgg agtggcggcc ctcggcgcgt tcgtactgtt ccacgatggt gtagtcctcg
     4261 ttgtgggagg tgatgtccaa cttgatgttg acgttgtagg cgccgggcag ctgcacgggc
     4321 ttcttggcct tgtaggtggt cttgacctca gcgtcgtagt ggccgccgtc cttcagcttc
     4381 agcctctgct tgatctcgcc cttcagggcg ccgtcctcgg ggtacatccg ctcggaggag
     4441 gcctgccagc ccatggtctt cttctgcatt acggggccgt cggaggggaa gttggtgccg
     4501 cgcagcttca ccttgtagat gaactcgccg tcctgcaggg aggagtcctg ggtcacggtc
     4561 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
     4621 agcttcaagt agtcggggat gtcggcgggg tgcttcacgt aggccttgga gccgtacatg
     4681 aactgagggg acaggatgtc ccaggcgaag ggcagggggc cacccttggt caccttcagc
     4741 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
     4801 tggccgttca cggagccctc catgtgcacc ttgaagcgca tgaactcctt gatgatggcc
     4861 atgttatcct cctcgccctt gctcaccatg gtggcgatcc ggtagcgcta gcgatgagct
     4921 ctggtagtag actagtggcc cccattatat accctctaga ggatcctatc cactccgtgt
     4981 aagggagagt gagcctctta cgaatctacg gctacttcga cgtctttccg aggcgactga
     5041 tacctacggc tacttcgacg tcttacgaag gcagtccgat tctacggcta cttcgacgtc
     5101 tacctttact gagacgggac tacggctact tcgacgtcta aggcgttgcg aatcctcatg
     5161 cgattgttac gaaacccgct acggctactt cgacgtctta ttggaagcac ggctctctac
     5221 ggctacttcg acgtctacct cgggagaact gattctacgg ctacttcgac gtctaagagg
     5281 ttgacgctat ccctacggct acttcgacgt ctagccctgt aggtagacta gatacagtgt
     5341 tactcaatcg tccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     5401 aagaccccac ctgtaggttt ggcaagctag ctgcagtaac gccattttgc aaggcatgga
     5461 aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga aaatagctaa
     5521 cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg ggccaagaac
     5581 agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc cccagatatg
     5641 gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc ccaaggacct
     5701 gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct tctgttcgcg
     5761 cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc gcgccagtcc
     5821 tccgacagac tgagtcgccc gggggggatc tggagctctc gagaattctc acgcgtgccg
     5881 ccaccatgtc tagaccaggc gaacgaccgt ttcaatgccg gatatgtatg aggaacttct
     5941 cccagaggtc cagcttggta cggcacaccc gaacacatac aggagaaaag ccattccaat
     6001 gtcgaatttg tatgcgcaat ttttcagaca agtctgtgtt ggctcgacac ctccgcacac
     6061 atactggttc acagaagccc tttcagtgca ggatttgtat gcgcaacttt agccaacgct
     6121 catccctggt aaggcacact agaacgcata caggtgagaa gcctttccag tgtcgcatct
     6181 gtatgcggaa cttcagccag cggaataact tgggcaggca tttgcgaact cacaccggtt
     6241 ctcaaaaacc ttttcagtgc cgaatttgta tgcgcaactt cagcacccac gccgtattga
     6301 cacgacacac gcgcacgcac accggggaaa aaccgtttca atgtcgaatc tgcatgcgca
     6361 attttagcga tagagggaac ctcactcgac atctgcgcac acacttgcgg ggatccacct
     6421 gcagggatga gtttcccacc atggtgtttc cttctgggca gatcagccag gcctcggcct
     6481 tggccccggc ccctccccaa gtcctgcccc aggctccagc ccctgcccct gctccagcca
     6541 tggtatcagc tctggcccag gccccagccc ctgtcccagt cctagcccca ggccctcctc
     6601 aggctgtggc cccacctgcc cccaagccca cccaggctgg ggaaggaacg ctgtcagagg
     6661 ccctgctgca gctgcagttt gatgatgaag acctgggggc cttgcttggc aacagcacag
     6721 acccagctgt gttcacagac ctggcatccg tcgacaactc cgagtttcag cagctgctga
     6781 accagggcat acctgtggcc ccccacacaa ctgagcccat gctgatggag taccctgagg
     6841 ctataactcg cctagtgaca ggggcccaga ggccccccga cccagctcct gctccactgg
     6901 gggccccggg gctccccaat ggcctccttt caggagatga agacttctcc tccattgcgg
     6961 acatggactt ctcagccctg ctgagtcaga tcagctccca attgtgcgta cgcggatcct
     7021 ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta
     7081 agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg
     7141 ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga
     7201 tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga
     7261 agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg
     7321 cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc
     7381 tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg
     7441 tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg
     7501 gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc
     7561 tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga
     7621 tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc
     7681 agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca
     7741 tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg
     7801 aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg
     7861 agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt
     7921 attacatcac gggggaggca gagggtttcc ctgccacagc ttaagcggcc gcgactctag
     7981 agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
     8041 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8101 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8161 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     8221 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     8281 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     8341 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8401 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8461 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8521 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     8581 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
     8641 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     8701 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     8761 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     8821 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     8881 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     8941 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     9001 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     9061 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     9121 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     9181 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     9241 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     9301 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     9361 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     9421 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     9481 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     9541 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     9601 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     9661 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     9721 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     9781 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     9841 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     9901 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     9961 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
    10021 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
    10081 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
    10141 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
    10201 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
    10261 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
    10321 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
    10381 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
    10441 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
    10501 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
    10561 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
    10621 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
    10681 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
    10741 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
    10801 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
    10861 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
    10921 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
    10981 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
    11041 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
    11101 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
    11161 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
    11221 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
    11281 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
    11341 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    11401 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    11461 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    11521 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    11581 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    11641 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    11701 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
    11761 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    11821 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    11881 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
    11941 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
    12001 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
    12061 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
    12121 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
    12181 gtgaaatact ggtttttagt gcgccagatc tctataa
//
