LOCUS       sequence-384539-        2710 bp    DNA     circular SYN 15-APR-2025
DEFINITION  pENTRY clone for NLR Integrated Domain ID#58.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Landry D, Mila I, Sabbagh CRR, Zaffuto M, Pouzet C, Tremousaygue D, 
            Dabos P, Deslandes L, Peeters N
  TITLE     An NLR integrated domain toolkit to identify plant pathogen effector
            targets.
  JOURNAL   Plant J. 2023 Sep;115(5):1443-1457. doi: 10.1111/tpj.16331. Epub 
            2023 Jul 24.
  PUBMED    37248633
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.16331";
            journalName: "Plant J"; date: "2023-09"; volume: "115"; issue: "5"; 
            pages: "1443-1457"
COMMENT     Sequence Label: pIM42
FEATURES             Location/Qualifiers
     source          1..2710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    152..251
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(597..696)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(753..769)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(753..769)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(766..788)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(941..1529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1021..1040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1665..2480)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1665..2480)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2388..2407
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2472..2491
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2601..2617
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2601..2617
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     terminator      2618..2664
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aacaaaaccc atcgtacggc caaggaagtc tccaataact gtgatccacc acaagcgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtc atgcataatc cgcacgcatc
      121 tggaataagg aagtgccatt ccgcctgacc tcaaataatg attttatttt gactgatagt
      181 gacctgttcg ttgcaacaga ttgatgagca atgctttttt ataatgccaa ctttgtacaa
      241 aaaagcaggc ttcgaaggag atagaaccat gagcacccat ggagcagagc gggtagacaa
      301 aacatctagg ggtgctgctg cggctgcgga tatacagaac agcagcggtg gcaatttacg
      361 gtccaagcat tggaaagact tcacggtcat aacatgggag agtggaaagg cggtcgaagc
      421 aaaatgtaat ctctgcggag ctcacccgat caagtgcgga agcggcaacg ggacatcagt
      481 tttgagcaat catctcaaga gtaaaggttg catcaggaaa cgtagaggac cgccaaaccc
      541 ttcaagcacc ggtgatgata ctgaaattgc gaggcatatt gttattggtg attcagaccc
      601 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
      661 acaggtcact atcagtcaaa ataaaatcat tatttgaggc taggtggagg ctcagtgatg
      721 ataagtctgc gatggtggat gcatgtgtca tggtcatagc tgtttcctgt gtgaaattgt
      781 tatccgctca gagggcacaa tcctattccg cgctatccga caatctccaa gacattaggt
      841 ggagttcagt tcggcgtatg gcatatgtcg ctggaaagaa catgtgagca aaaggccagc
      901 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      961 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     1021 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     1081 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     1141 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     1201 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     1261 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     1321 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     1381 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     1441 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     1501 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     1561 acgctctatt caacaaagcc gccgtcccgt caagtcagcg taaatgggta gggggcttca
     1621 aatcgtccgc tctgccagtg ttacaaccaa ttaacaaatt ctgattagaa aaactcatcg
     1681 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     1741 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     1801 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     1861 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     1921 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     1981 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2041 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2101 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2161 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2221 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2281 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2341 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     2401 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt
     2461 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     2521 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     2581 acgtggcttt cccccgccgc tctagaacta gtggatccaa ataaaacgaa aggctcagtc
     2641 gaaagactgg gcctttcgtt ttatctgttg tttgtcgcat tatacgagac gtccaggttg
     2701 ggatacctga
//
