LOCUS       sequence-384649-       10778 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses mouse SAFB lacking the third computationally called 
            disordered domain in mammalian cells. Contains a c-terminal 3xFlag 
            tag and a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10778)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 10778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-safb∆dd3-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..10778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5093..5113
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5120..5185
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5204..5245
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    5275..5499
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5627..5717
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        5765..5976
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5989..6257
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             6294..7319
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7348..7469
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7385..7404)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7439..7458
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8232..8266
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8757..8773)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8757..8773)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8770..8792)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8781..8797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8805..8811,8812..8829,8830..8835))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8850..8871
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8988..9005)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9159..9747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9239..9258)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9918..10709,10710..10778))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10541..10560
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1861 gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggsgrggcg
     1921 grgaggkgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggatc ggtactgttg gtaaagccac
     3121 catggcggag actctgtcgg gtttaggaga cgcgagtgcg gcgggcgcgg cggccgtgag
     3181 ctccgcggcg tcagagaccg ggacgcggcg gctcagcgac ctgcgggtga tcgacctgcg
     3241 ggcggagctg aagaagcgga acctcgactc gagcggcaac aagagcgtgc tgatggagcg
     3301 gctgaagaag gccattgaag atgaaggtgg gaatcctgat gaaattgaag tcacctcaga
     3361 atgcaacaag aagatgccta agaggcccag caaagggagg aaaccagaag acgagggtgt
     3421 ggaggacaac gggctcgagg agaattctgg ggatgggcag gaggatgtgg agaccagcct
     3481 ggagaacctg caagacatgg atatgatgga catcagtgtg ctggatgagg ctgacattga
     3541 caatggcagc gtggcagact gtgtggagga agaggaggag gccacactcc ctgagggcct
     3601 agcagacagc actgaactgg tagaaggaga tttgaaaggg ctcccagaac agctgcagga
     3661 gcatgctata gatgacaagg acaccgttaa caatgtagat acgtcatcat ctgacttcac
     3721 catgctgcag gagatggaag aggcatcttt ggagccagaa aatgagaaaa tactcgacat
     3781 tttgggggaa acttgtaaat ctgagccagt aaaagaagaa ggttcggagc tggagcagcc
     3841 ctttgcccag gccacgagta gcgtggggcc agacaggaag ctggcggagg aagaggacct
     3901 atttgagagc tgcggccacc cggaagagga agaggaagag gaggaagaag atcaggaaga
     3961 ggagcaggag gaggagggag atttagcttt ggccagcagc agcaagtctg agtctccaag
     4021 cactcggtgt cagtggagcg aggcagatgc cccgttagca gtagtgaaaa gggagccggc
     4081 agatgcgcct ggcggaggca ctgggatgga ccgcgagcct gtagggctag aggagccagt
     4141 tgaacagagt agcacggctg cccagctccc ggaggccacc agccaggagc tggtgcgagc
     4201 gcccacggca gccctgagcc ctgagcccca agatagcaaa gaagacgtga agaagtttgc
     4261 ttttgacgct tgtaatgacg tccctgcgcc tcctaaagag tcctcagcca gtgagggcgc
     4321 tgatcagaaa atgagttctg tggaggagga ctcggataca aaaaggcttt cccgagaaga
     4381 gaagggtcgc agcagctgtg gtaggaattt ctgggttagt ggcctttcgt ctacaaccag
     4441 agccacggat ctgaagaacc tgtttagcag atatgggaag gtggtgggtg ctaaggttgt
     4501 gacgaacgcc cggagtcctg gagctcgctg ttatggcttt gtcactatgt ccacagctga
     4561 agaggcaaca aaatgcatta gccatttgca caagactgag ctgcatggca agatgatctc
     4621 agtggaaaag gccaaaagtg agcccactgg caaaagagtg cctgacaggc gagatgggga
     4681 cagcaagaag gagaaggcca gcaccagtga cagatctgca aaccttaaga gggaggagaa
     4741 aggtgagaga aaagacgatg ccaagaagac agatgatggg agcacagaga agagcaaaga
     4801 cgcagatgac cagaaacctg ggccttcaga gcgctcgcga acgaccaagt caggaagccg
     4861 tggcactgag cgcactgtgg tgatggacaa gtccaaaggt gtgcctgtca tcagtgtgaa
     4921 aacatctggg tctaaagaga gagcctccaa aagtcaggac cggaagtcag ccagccggga
     4981 gaagcggtct gttgtgtcct ttgacaaagt taaagagtct cgaaagtcca gagacagcag
     5041 acccagtgat gcccgcttca cccgccgcta tggccctggt accgcggccg ctcccaagaa
     5101 gaagaggaaa gtctcgagcg attacaaaga ccatgacggt gattataaag atcatgacat
     5161 cgactacaag gatgacgatg acaagggctc gggctcgacc tcgggtaagc ctatccctaa
     5221 ccctctcctc ggtctcgatt ctacatgaaa tctagagtcg acaatgcgtt tcgactgtgc
     5281 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5341 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5401 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5461 acaatagcag gcatgctggg gatgcggtgg gctctatgga aacacgcggc gacgcgttcg
     5521 accgaataaa acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg
     5581 ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa
     5641 gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttgt
     5701 cgagattttc aggagctaaa gatctggaag ctaaggatct gcgatcgctc cggtgcccgt
     5761 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5821 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5881 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5941 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
     6001 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
     6061 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
     6121 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
     6181 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
     6241 ctgcgccgtt acagatccaa gctgtgaccg gcgcctacaa gctgctagcc accatgaaaa
     6301 agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct
     6361 ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag
     6421 ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg
     6481 tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt
     6541 tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc
     6601 tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg
     6661 ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc
     6721 aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc
     6781 aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc
     6841 tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca
     6901 atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg
     6961 gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg
     7021 agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc
     7081 gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt
     7141 tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga
     7201 ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag
     7261 aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagt
     7321 tttacgtatt gagtatttaa ctaactaaac ttgtttattg cagcttataa tggttacaaa
     7381 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     7441 ggtttgtcca aactcatcaa tgtatcttat catgtctgga attgactcaa atgatgtcaa
     7501 ttagtctatc agaagctcat ctggtctccc ttccggggga caagacatcc ctgtttaata
     7561 tttaaacagc agtgttccca aactgggttc ttatatccct tgctctggtc aaccaggttg
     7621 cagggtttcc tgtcctcaca ggaacgaagt ccctaaagaa acagtggcag ccaggtttag
     7681 ccccggaatt gactggattc cttttttagg gcccattggt atggcttttt ccccgtatcc
     7741 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
     7801 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
     7861 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
     7921 attacatccc tgggggcttt gggggggggc tgtccctgat atctataaca agaaaatata
     7981 tatataataa gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt
     8041 aaatcttaaa agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata
     8101 gttcaaaatc agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc
     8161 gactgagatg tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata
     8221 tttcaagaat gcatgcgtca attttacgca gactatcttt ctagggttaa tctagctgca
     8281 tcaggatcat atcgtcgggt cttttttccg gctcagtcat cgcccaagct ggcgctatct
     8341 gggcatcggg gaggaagaag cccgtgcctt ttcccgcgag gttgaagcgg catggaaaga
     8401 gtttgccgag gatgactgct gctgcattga cgttgagcga aaacgcacgt ttaccatgat
     8461 gattcgggaa ggtgtggcca tgcacgcctt taacggtgaa ctgttcgttc aggccacctg
     8521 ggataccagt tcgtcgcggc ttttccggac acagttccgg atggtcagcc cgaagcgcat
     8581 cagcaacccg aacaataccg gcgacagccg gaactgccgt gccggtgtgc agattaatga
     8641 cagcggtgcg gcgctgggat attacgtcag cgaggacggg tatcctggct ggatgccgca
     8701 gaaatggaca tggatacccc gtgagttacc cggcgggcgc gcttggcgta atcatggtca
     8761 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8821 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8881 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8941 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9001 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9061 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9121 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9181 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9241 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9301 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9361 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9421 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9481 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9541 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9601 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9661 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9721 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9781 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9841 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9901 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9961 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10021 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10081 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10141 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10201 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10261 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10321 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10381 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10441 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10501 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10561 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10621 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10681 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10741 aagggaataa gggcgacacg gaaatgttga atactcat
//
