LOCUS       sequence-384675-       11534 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses mouse SAFB lacking the n-terminal SAP domain in mammalian 
            cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Cherney R, Eberhard Q, Giri G, Mills C, Porrello A, Zhang Z, White 
            D, Trotman JB, Herring L, Dominguez D, Calabrese M
  TITLE     SAFB associates with nascent RNAs and can promote gene expression in
            mouse embryonic stem cells.
  JOURNAL   RNA. 2023 Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122.
  PUBMED    37468167
REFERENCE   2  (bases 1 to 11534)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2023 
            Jul 19:rna.079569.122. doi: 10.1261/rna.079569.122."
COMMENT     Sequence Label: CAGGS-safb∆sap-NLS-3XFLAG-V5
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1444..1747
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2027..3043
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2967..2989
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3051..3070
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3116..3125
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5849..5869
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5876..5941
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5960..6001
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    6031..6255
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6383..6473
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        6521..6732
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             6745..7013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             7050..8075
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    8104..8225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8141..8160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8195..8214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8988..9022
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(9513..9529)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9513..9529)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9526..9548)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9537..9553
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9561..9567,9568..9585,9586..9591))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9606..9627
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9744..9761)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9915..10503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9995..10014)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10674..11465,11466..11534))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11297..11316
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc acggccagac tagtggatcc attgattatt
     1381 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     1441 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     1501 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     1561 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     1621 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1681 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1741 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
     1801 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
     1861 gggggggggg cgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggsgrggsg
     1921 grgrggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     1981 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     2041 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     2101 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     2161 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     2221 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     2281 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     2341 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     2401 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     2461 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     2521 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     2581 cgtgccgggc ggggggtggc ggcaggkggg ggtgccgggc ggggcggggc cgcckcgggc
     2641 cggggrgggc tcgggggrgg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     2701 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     2761 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     2821 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     2881 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     2941 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     3001 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     3061 ggttattgtg ctgtctcatc attttggcaa acctaggatc ggtactgttg gtaaagccac
     3121 catggcggag actctgtcgg gtttaggaga cgcgagtgcg gcgggcgcgg cggccgtgag
     3181 ctccgcggcg tcagagaccg ggacgcggcg ggaagatgaa ggtgggaatc ctgatgaaat
     3241 tgaagtcacc tcagaatgca acaagaagat gcctaagagg cccagcaaag ggaggaaacc
     3301 agaagacgag ggtgtggagg acaacgggct cgaggagaat tctggggatg ggcaggagga
     3361 tgtggagacc agcctggaga acctgcaaga catggatatg atggacatca gtgtgctgga
     3421 tgaggctgac attgacaatg gcagcgtggc agactgtgtg gaggaagagg aggaggccac
     3481 actccctgag ggcctagcag acagcactga actggtagaa ggagatttga aagggctccc
     3541 agaacagctg caggagcatg ctatagatga caaggacacc gttaacaatg tagatacgtc
     3601 atcatctgac ttcaccatgc tgcaggagat ggaagaggca tctttggagc cagaaaatga
     3661 gaaaatactc gacattttgg gggaaacttg taaatctgag ccagtaaaag aagaaggttc
     3721 ggagctggag cagccctttg cccaggccac gagtagcgtg gggccagaca ggaagctggc
     3781 ggaggaagag gacctatttg agagctgcgg ccacccggaa gaggaagagg aagaggagga
     3841 agaagatcag gaagaggagc aggaggagga gggagattta gctttggcca gcagcagcaa
     3901 gtctgagtct ccaagcactc ggtgtcagtg gagcgaggca gatgccccgt tagcagtagt
     3961 gaaaagggag ccggcagatg cgcctggcgg aggcactggg atggaccgcg agcctgtagg
     4021 gctagaggag ccagttgaac agagtagcac ggctgcccag ctcccggagg ccaccagcca
     4081 ggagctggtg cgagcgccca cggcagccct gagccctgag ccccaagata gcaaagaaga
     4141 cgtgaagaag tttgcttttg acgcttgtaa tgacgtccct gcgcctccta aagagtcctc
     4201 agccagtgag ggcgctgatc agaaaatgag ttctgtggag gaggactcgg atacaaaaag
     4261 gctttcccga gaagagaagg gtcgcagcag ctgtggtagg aatttctggg ttagtggcct
     4321 ttcgtctaca accagagcca cggatctgaa gaacctgttt agcagatatg ggaaggtggt
     4381 gggtgctaag gttgtgacga acgcccggag tcctggagct cgctgttatg gctttgtcac
     4441 tatgtccaca gctgaagagg caacaaaatg cattagccat ttgcacaaga ctgagctgca
     4501 tggcaagatg atctcagtgg aaaaggccaa aagtgagccc actggcaaaa gagtgcctga
     4561 caggcgagat ggggacagca agaaggagaa ggccagcacc agtgacagat ctgcaaacct
     4621 taagagggag gagaaaggtg agagaaaaga cgatgccaag aagacagatg atgggagcac
     4681 agagaagagc aaagacgcag atgaccagaa acctgggcct tcagagcgct cgcgaacgac
     4741 caagtcagga agccgtggca ctgagcgcac tgtggtgatg gacaagtcca aaggtgtgcc
     4801 tgtcatcagt gtgaaaacat ctgggtctaa agagagagcc tccaaaagtc aggaccggaa
     4861 gtcagccagc cgggagaagc ggtctgttgt gtcctttgac aaagttaaag agtctcgaaa
     4921 gtccagagac tcagagtctc ggagggaacg ggagcgcagt gagcgggagc agaggctgca
     4981 ggcccagtgg gagcgggagg agcgagagcg gctggagatt gctcgtgagc gcttggcatt
     5041 ccaccgccat cggctagagc gtgaacgcat ggagcgggag cggctggagc gggagcggat
     5101 gcatgtagaa caggagcgga ggcgtgagca agagcgcatt caccgagagc gagaggagct
     5161 tcggcgtcag caggagctgc gctatgaaca ggagcgccgg cctgctgtgc gcaggcccta
     5221 tgaagtggat ggccggcgag atgatgccta ttggccagaa gctaaacggg ctgctctgga
     5281 tgaccgttac cactcagact tcagccgcca ggaccgcttt cacgacttcg accacagaga
     5341 cagaggccgt tatcccaacc attctgtgga taggagagaa ggttcacggt cgatgatggg
     5401 agacagagaa ggacagcatt accctgaacg ccatggagga cctgaacgcc atggccggga
     5461 ctctcgagat ggctggggct atggttcgaa caagagattg agtgaaggcc gggggctgcc
     5521 ccctcctccc aggcgtgact ggggggagca tggccggcga cttgaagatg accgggcatg
     5581 gcagggcacg gctgatggag gcatgatgga gcgtgatcat aagaggtggc aaggtggtga
     5641 gaggagcatg tctggccact cggggcctgg tcacatgatg aaccgtggtg gcatgtcagg
     5701 gcgaggcagt tttgccccag gaggagcttc tcgtggccat gtgatcccac gtggagggat
     5761 gcaggcaggc tttggaggac agagccgggg aagcagaccc agtgatgccc gcttcacccg
     5821 ccgctatggc cctggtaccg cggccgctcc caagaagaag aggaaagtct cgagcgatta
     5881 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
     5941 gggctcgggc tcgacctcgg gtaagcctat ccctaaccct ctcctcggtc tcgattctac
     6001 atgaaatcta gagtcgacaa tgcgtttcga ctgtgccttc tagttgccag ccatctgttg
     6061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6241 cggtgggctc tatggaaaca cgcggcgacg cgttcgaccg aataaaacct gtgacggaag
     6301 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
     6361 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
     6421 aaataagatc actaccgggc gtattttttg agttgtcgag attttcagga gctaaagatc
     6481 tggaagctaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg cacatcgccc
     6541 acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta gagaaggtgg
     6601 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     6661 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     6721 gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc ccgccgccct
     6781 acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc ctgtggtgcc
     6841 tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc gggcctttgt
     6901 ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg cctgaccctg
     6961 cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag atccaagctg
     7021 tgaccggcgc ctacaagctg ctagccacca tgaaaaagcc tgaactcacc gcgacgtctg
     7081 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     7141 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     7201 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     7261 cgctcccgat tccggaagtg cttgacattg gggagttcag cgagagcctg acctattgca
     7321 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     7381 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     7441 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     7501 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     7561 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     7621 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     7681 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     7741 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     7801 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     7861 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     7921 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     7981 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     8041 gacgccccag cactcgtccg agggcaaagg aatagtttta cgtattgagt atttaactaa
     8101 ctaaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8161 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8221 tcttatcatg tctggaattg actcaaatga tgtcaattag tctatcagaa gctcatctgg
     8281 tctcccttcc gggggacaag acatccctgt ttaatattta aacagcagtg ttcccaaact
     8341 gggttcttat atcccttgct ctggtcaacc aggttgcagg gtttcctgtc ctcacaggaa
     8401 cgaagtccct aaagaaacag tggcagccag gtttagcccc ggaattgact ggattccttt
     8461 tttagggccc attggtatgg ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     8521 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     8581 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     8641 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
     8701 gggggctgtc cctgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
     8761 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
     8821 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
     8881 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
     8941 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
     9001 tacgcagact atctttctag ggttaatcta gctgcatcag gatcatatcg tcgggtcttt
     9061 tttccggctc agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg
     9121 tgccttttcc cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg
     9181 cattgacgtt gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca
     9241 cgcctttaac ggtgaactgt tcgttcaggc cacctgggat accagttcgt cgcggctttt
     9301 ccggacacag ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga
     9361 cagccggaac tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta
     9421 cgtcagcgag gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga
     9481 gttacccggc gggcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     9541 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     9601 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     9661 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9721 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10081 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10321 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    10381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10681 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10741 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10801 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10861 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10921 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10981 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    11041 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    11101 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    11161 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    11221 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    11281 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    11341 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    11401 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    11461 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    11521 tgttgaatac tcat
//
