LOCUS       sequence-384861-        4497 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus BVRF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-BVRF1
FEATURES             Location/Qualifiers
     source          1..4497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2376..2475)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2492..2511)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2493..2511)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2516..2532)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2516..2532)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2963..3754
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3847..4435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4336..4355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg gcgctctccg ggcacgtctt gatagacccc gcccgactgc ccagggacac
      721 aggccccgaa ctcatgtggg ccccaagcct tcgcaactca ctgcgcgtgt cccccgaagc
      781 gctcgagctg gcagagcggg aggccgaaag ggccaggtcg gagcggtggg acaggtgtgc
      841 ccaggtgctc aaaaataggc tgctccgcgt ggagctggac ggcatcatgc gtgaccacct
      901 ggccagggcg gaggagatcc gccaggacct ggatgctgta gtggccttct ctgatggcct
      961 ggagagcatg caggtcaggt ccccctccac gggagggcgc tctgcgccag ccccgccctc
     1021 cccatcccca gcccagccgt tcactcggct caccgggaac gcccagtatg cagtctcaat
     1081 ctctcccacg gacccccctc tgatggtggc cggcagcctg gctcaaacgc tgcttggtaa
     1141 tctgtacggg aacatcaacc agtgggtacc gtccttcgga ccctggtaca ggaccatgtc
     1201 ggctaatgcc atgcagcggc gcgtgttccc taagcagctg aggggcaacc tgaactttac
     1261 caactccgtc tccctaaagc tgatgacaga agtggtggcg gtgcttgagg gcaccaccca
     1321 ggactttttc tcagacgtca ggcacctgcc agacctccag gctgccctga tcctctcggt
     1381 ggcctacctg ctactccagg ggggctcctc acaccagcag cgccccctcc ctgcctcacg
     1441 ggaagagctg ctggagctgg gcccggagag cctagagaaa atcatcgccg acctcaaggc
     1501 caagtcaccc ggcggaaatt ttatgatttt aacaagcgga aacaaggaag cgcgccagtc
     1561 aatagcccct ctcaaccgac aggcggcata tccacccggc acattcgcgg acaataagat
     1621 ttacaacctg tttgtgggag cgggactact gcccacgacg gccgcgctga acgtgcccgg
     1681 ggcggcgggt cgggaccggg acctggtgta ccggatcgcc aaccagatct ttggggagga
     1741 tgtgcccccc ttctcatctc accagtggaa cctgcgcgta ggtttagccg cactcgaggc
     1801 cctgatgctc gtctacacgc tctgcgagac cgccaacctg gccgaggcgg ccacccggcg
     1861 tctacaccta tcgtccctgc tcccccaggc aatgcagcgg cgcaagcctg ccatggcgtc
     1921 agctggtatg ccgggcgcct atccagtcca gacgcttttc cgccacgggg agctcttccg
     1981 cttcatctgg gcccactacg tgaggcccac ggtggcggca gacccccagg cctccatcag
     2041 ctctcttttc cccgggctgg ttttgctggc cctggagctg aagttgatgg atgggcaggc
     2101 tccctcccat tatgccataa acctgaccgg acaaaagttt gacaccctct ttgagattat
     2161 caaccagaag cttttatttc acgacccggc tgccatgctg gcggcgcgca cacagctgcg
     2221 tctagccttc gaggacggcg tcggtgttgc cctggggcgc ccctcgccca tgcttgcggc
     2281 gcgggagatc ctggagcgtc agttctcagc ctcggatgac tacgaccggc tgtacttcct
     2341 gacgctgggc tacctggcct ccccggtggc cccaagccca actttcttgt acaaagttgg
     2401 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2461 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2521 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2581 taaaaatata tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct
     2641 gctacccaag gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg
     2701 gacataagcc tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg
     2761 gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg
     2821 ttatgactgt ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta
     2881 cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg
     2941 ccctaaaaca aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact
     3001 atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt
     3061 gtacggctcc gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac
     3121 ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac
     3181 ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca
     3241 cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca
     3301 gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat
     3361 cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact
     3421 ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg
     3481 gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat
     3541 ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat
     3601 ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg
     3661 acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt
     3721 gaaaggcgag atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc
     3781 ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3841 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3901 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3961 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     4021 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     4081 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     4141 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     4201 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     4261 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     4321 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     4381 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     4441 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
