LOCUS       sequence-384908-         970 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  evolved sortase A (eSrtA) pentamutant with improved kinetics and 
            activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Chen I, Dorr BM, Liu DR
  TITLE     A general strategy for the evolution of bond-forming enzymes using 
            yeast display.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jul 12;108(28):11399-404. doi: 
            10.1073/pnas.1101046108. Epub 2011 Jun 22.
  PUBMED    21697512
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1101046108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-07-12- 12"; volume: "108"; issue: "28"; pages: 
            "11399-404"
COMMENT     Sequence Label: sortase A pentamutant (eSrtA) in pET29
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             484..501
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(554..572)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      568..615
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(739..758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     949..970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgcaag ctaaacctca aattccgaaa
       61 gataaatcaa aagtggcagg ctatattgaa attccagatg ctgatattaa agaaccagta
      121 tatccaggac cagcaacacg cgaacaatta aatagaggtg taagctttgc agaagaaaat
      181 gaatcactag atgatcaaaa tatttcaatt gcaggacaca ctttcattga ccgtccgaac
      241 tatcaattta caaatcttaa agcagccaaa aaaggtagta tggtgtactt taaagttggt
      301 aatgaaacac gtaagtataa aatgacaagt ataagaaacg ttaagccaac agctgtagaa
      361 gttctagatg aacaaaaagg taaagataaa caattaacat taattacttg tgatgattac
      421 aatgaagaga caggcgtttg ggaaacacgt aaaatctttg tagctacaga agtcaaactc
      481 gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa ggaagctgag
      541 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
      601 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
      661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      841 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
      901 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
      961 tccaaactgg
//
