LOCUS       sequence-384910-        4710 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Gateway Entry Clone of Epstein-Barr virus EBNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Calderwood MA, Venkatesan K, Xing L, Chase MR, Vazquez A, Holthaus 
            AM, Ewence AE, Li N, Hirozane-Kishikawa T, Hill DE, Vidal M, Kieff 
            E, Johannsen E
  TITLE     Epstein-Barr virus and virus human protein interaction maps.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7606-11. doi: 
            10.1073/pnas.0702332104. Epub 2007 Apr 19.
  PUBMED    17446270
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0702332104"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2007-05-1- 1"; volume: "104"; issue: "18"; pages: "7606-11"
COMMENT     Sequence Label: pENTR223-EBNA1
FEATURES             Location/Qualifiers
     source          1..4710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             668..2590
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    complement(2589..2688)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2705..2724)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2706..2724)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2729..2745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2729..2745)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3176..3967
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4060..4648
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4549..4568
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcatg tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg
      721 agacacatct ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa
      781 ccatggacga ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg
      841 cggctcagga tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag
      901 ttgcattggc tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc
      961 aggagcagga ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc
     1021 aggaggggca ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg
     1081 ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg
     1141 agcaggagga ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
     1201 aggaggggca ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc
     1261 aggaggaggg gcaggagcag gaggggcagg aggggcagga ggggcaggag caggaggggc
     1321 aggagcagga ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg
     1381 aggggcagga gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc
     1441 aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc
     1501 aggagcagga ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg
     1561 aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg
     1621 agcaggaggg gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg
     1681 tcgaggaggt agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg
     1741 tgaaagagcc agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga
     1801 aaagaggccc aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc
     1861 ccctccaggt agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca
     1921 ccaagaaggt ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc
     1981 cgcagatgac ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag
     2041 gcgcaaaaaa ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt
     2101 tgagaacatt gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac
     2161 cgacgaagga acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta
     2221 caacctaagg cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg
     2281 tctccccttt ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat
     2341 tgtctgttat ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc
     2401 gattaaggac cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg
     2461 cagctttgac gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc
     2521 cgcggagggt gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga
     2581 agggcaggag ccaactttct tgtacaaagt tggcattata agaaagcatt gcttatcaat
     2641 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga
     2701 tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt
     2761 gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat gcctcctcta
     2821 gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg ggtgacgcac
     2881 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     2941 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     3001 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3061 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3121 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acattatgag
     3181 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     3241 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     3301 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     3361 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     3421 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     3481 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     3541 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     3601 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     3661 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     3721 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     3781 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     3841 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     3901 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     3961 caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     4021 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4081 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4141 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4201 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4261 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4321 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4381 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4441 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4501 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4561 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4621 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4681 ggccttttgc tggccttttg ctcacatgtt
//
