LOCUS       sequence-385558-       11752 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of ShCAST under control of lacI Plac promoter/repressor 
            pair. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   Trujillo Rodriguez L, Ellington AJ, Reisch CR, Chevrette MG
  TITLE     CRISPR-Associated Transposase for Targeted Mutagenesis in Diverse 
            Proteobacteria.
  JOURNAL   ACS Synth Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065.
  PUBMED    37368499
REFERENCE   2  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Jun 27. doi: 10.1021/acssynbio.3c00065."
COMMENT     Sequence Label: pBLlSh2
FEATURES             Location/Qualifiers
     source          1..11752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             446..1108
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     1802..1824
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1816..1833
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1817..1833
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1884..2966)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2928..2947
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2967..3044)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      complement(3108..3151)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3154..3175
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     protein_bind    3178..3194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3201..3217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3265..3276
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      8694..8765
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(9152..9946)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9263..9282)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9873..9892
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      join(11426..11752,1..445)
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 tgggggcagt cgaagggcga agcccgcccg cctgcccccc gagcctcacg gcggcgagtg
       61 cgggggttcc aagggggcag cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa
      121 caagccccgg aggggccact ttttgccgga gggggagccg cgccgaaggc gtgggggaac
      181 cccgcagggg tgcccttctt tgggcaccaa agaactagat atagggcgaa atgcgaaaga
      241 cttaaaaatc aacaacttaa aaaagggggg tacgcaacag ctcattgcgg caccccccgc
      301 aatagctcat tgcgtaggtt aaagaaaatc tgtaattgac tgccactttt acgcaacgca
      361 taattgttgt cgcgctgccg aaaagttgca gctgattgcg catggtgccg caaccgtgcg
      421 gcacccctac cgcatggaga taagcatggc cacgcagtcc agagaaatcg gcattcaagc
      481 caagaacaag cccggtcact gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg
      541 gcttattgcg aggaaaccca cggcggcaat gctgctgcat cacctcgtgg cgcagatggg
      601 ccaccagaac gccgtggtgg tcagccagaa gacactttcc aagctcatcg gacgttcttt
      661 gcggacggtc caatacgcag tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa
      721 gctcaacggc cccggcaccg tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca
      781 gccccgcgac cagttgcgcc tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga
      841 ccaggacgaa tcgctgttgg ggcatggcga cctgcgccgc atcccgaccc tgtatccggg
      901 cgagcagcaa ctaccgaccg gccccggcga ggagccgccc agccagcccg gcattccggg
      961 catggaacca gacctgccag ccttgaccga aacggaggaa tgggaacggc gcgggcagca
     1021 gcgcctgccg atgcccgatg agccgtgttt tctggacgat ggcgagccgt tggagccgcc
     1081 gacacgggtc acgctgccgc gccggtagca cttgggttgc gcagcaaccc gtaagtgcgc
     1141 tgttccagac tatcggctgt agccgcctcg ccgccctata ccttgtctgc ctccccgcgt
     1201 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
     1261 taacggattc accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     1321 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     1381 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     1441 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     1501 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     1561 taaaaaaatt acgccccgcc ctgccactca tcgcagtacg gcctattggt taaaaaatga
     1621 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttccat
     1681 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     1741 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     1801 tcccagtcac gacgttgtaa aacgacggcc agtgagatcc cggtgcctaa tgagtgagct
     1861 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     1981 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     2041 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     2101 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     2161 gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     2221 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     2281 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     2341 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     2401 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     2461 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     2521 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     2581 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     2641 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     2701 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     2761 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     2821 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     2881 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     2941 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     3001 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     3061 tcccttatgc gactcctgca ttaggaaatg gcgtatcacg aggcagaatt tcagataaaa
     3121 aaaatcctta gctttcgcta aggatgattt ctggaattcg cggccgcttc tagagaattg
     3181 tgagcggata acaattgaca ttgtgagcgg ataacaagat actgagcaca ccctgtagga
     3241 actgggaaga gcgcacgtat gttgaaagag gagaaaggat ctatgaacag tcagcaaaat
     3301 cctgatttag ctgttcatcc cttggcaatt cctatggaag gcttactagg agaaagtgct
     3361 acaactcttg agaagaatgt aattgccaca caactctcag aggaagccca agtaaagcta
     3421 gaggtaatcc aaagtttact ggaaccctgc gatcgcacaa cttatgggca aaagttgcgg
     3481 gaagcagcag agaaactaaa tgtatcgttg cgaacggtac aaaggttggt gaaaaactgg
     3541 gaacaagatg gcttagtcgg actcactcaa acaagtaggg ctgataaagg aaaacaccgc
     3601 attggtgagt tttgggaaaa cttcattacc aaaacctaca aggagggtaa caagggaagt
     3661 aaacgtatga cccctaaaca agttgctctc agagtcgagg ctaaagcccg tgaattaaaa
     3721 gactctaagc cgcccaatta caaaaccgtg ttacgggtat tagcacccat tttggaaaag
     3781 caacaaaaag ccaagagtat ccgcagtcct ggttggagag gaactacgct ttcggttaaa
     3841 acccgtgaag gaaaagattt atcggttgat tacagtaacc atgtttggca atgtgaccat
     3901 acccgcgtgg atgtgttgct ggtagatcaa catggtgaaa ttttaagtcg tccctggcta
     3961 acaacagtaa ttgatactta ctctcgttgc attatgggta tcaacttggg ctttgatgca
     4021 cccagttctg gggtagtagc attagcgtta cgccatgcaa ttctaccaaa gcgttacggt
     4081 tccgagtaca aactgcattg tgagtgggga acctatggaa aaccagaaca tttttatact
     4141 gatggcggta aagactttcg ctctaaccac ttgagtcaga ttggggcgca attgggattt
     4201 gtctgtcatt tacgcgatcg cccttctgaa ggtggagtag tagaacgtcc cttcaaaaca
     4261 ttaaatgacc aactattttc aacgcttcct gggtacaccg gatctaatgt gcaggaacgc
     4321 ccagaagatg cagagaagga cgcaagactt actttgcgag aactagaaca gttacttgtg
     4381 cgttacatcg tagatcgtta caaccaaagt attgatgcgc ggatgggcga ccaaacgcgc
     4441 tttgagcgtt gggaagcagg attgcctaca gtgccagtac caataccaga acgagatttg
     4501 gatatttgtt taatgaagca gtcacggcgc actgtgcaaa gaggtggttg tttgcagttt
     4561 cagaatttaa tgtatcgggg ggaatatttg gcaggttatg ccggagaaac tgtcaactta
     4621 aggtttgacc ccagagacat tacaacaatt ttggtttatc gccaggaaaa caatcaggaa
     4681 gtatttctga ctcgcgctca cgctcaaggt ttggagacag agcaactggc attagatgag
     4741 gctgaggcag caagtcgcag actccgtacc gcagggaaaa ctatcagtaa ccaatcatta
     4801 ttgcaagaag ttgttgaccg cgatgctctt gtcgctacca agaaaagccg taaggagcgt
     4861 caaaaattgg aacagactgt tttgcgatct gctgctgttg atgaaagtaa tagagaatcc
     4921 ttgccttctc aaatagttga accagatgaa gtggaatcta cagaaacggt tcactctcaa
     4981 tacgaagaca ttgaggtgtg ggactatgaa caacttcgtg aagaatatgg gttttaaaca
     5041 atgacagaag ctcaggcgat cgccaagcag ttgggtgggg taaaaccgga tgatgagtgg
     5101 ttacaagctg aaattgctcg tctcaagggt aagagcattg tgcctttaca gcaggtaaaa
     5161 actctccatg attggttaga tggcaagcgc aaggcaagaa aatcttgccg agtagttggg
     5221 gaatcgagaa ctggcaagac agttgcttgt gatgcctaca gatacaggca caaacctcag
     5281 caggaagctg gacgacctcc aactgtgcct gtcgtttata ttcgacctca ccaaaaatgt
     5341 ggccccaagg atttgtttaa aaagattact gagtacctca agtatcgggt aacaaaaggg
     5401 actgtatctg attttcgaga taggacgata gaagtactca agggttgtgg cgtagagatg
     5461 ctaattattg atgaagctga ccgtctcaag cctgaaactt ttgctgatgt gcgagatatt
     5521 gccgaagatt taggaattgc tgtggtactg gtaggaacag accgtttgga tgcggtaatt
     5581 aagcgggatg agcaggttct cgaacgcttt cgggcgcatc ttcgctttgg taaattgtcg
     5641 ggagaggatt ttaagaacac cgtagaaatg tgggaacaaa tggttttgaa actgccagta
     5701 tcttctaatc taaagagcaa ggagatgcta cggattctca cgtcagcaac tgaaggctac
     5761 attggtcgcc ttgatgagat tcttagggaa gctgcaattc gttccttatc aagaggattg
     5821 aagaagattg acaaggctgt tttacaggaa gtagctaagg agtacaaatg atagaagcac
     5881 cagatgttaa accttggcta ttcttgatta aaccctatga aggggaaagc ctgagccact
     5941 ttcttggcag gttcagacgt gccaaccatt tatccgcaag tggattgggt actttggcag
     6001 gaattggtgc tatagtggca cgttgggaaa gatttcattt taatcctcgc cctagtcagc
     6061 aagaattgga agcgatcgca tctgtagtag aagtggatgc tcaaaggtta gcccagatgt
     6121 taccgcctgc tggagtggga atgcagcatg agccaattcg cttgtgtggg gcttgttatg
     6181 ccgagtcgcc ttgtcaccga attgaatggc agtacaagtc ggtgtggaag tgcgatcgcc
     6241 atcaactcaa gattttagca aagtgtccaa actgtcaagc accttttaaa atgcctgcgc
     6301 tgtgggagga tgggtgctgt cacagatgta ggatgccgtt tgcagaaatg gcaaagctac
     6361 agaaggtttg atgataaaac cagaaaaagg tgtgaaatta actaagtccc tgaattgatc
     6421 tggttgtcca aaaaatttgt gcgatcgcat ggcaagatta ttcctactat tgatgtggcg
     6481 tgagtcggat aacttgctct aatgggatcc cggttaatac caatagatca gatatgagta
     6541 ataaacatta cgacctttac ctgtggtatt cactgttggc acgacaacac ctttatcgcg
     6601 ccaatactgt agctgacgac gcgaacaatt agtgatttga gccgcttctg tgcttgtgta
     6661 gaattttcct tccatagaaa tcatcctaca gaagtagttt tttaaattta cattttttaa
     6721 aagcacatta tgtattcatg tgatattatt tttttatgag tcaaataact attcaagctc
     6781 gacttatttc ctttgaatca aaccgccaac aactctggaa gttgatggca gatttaaaca
     6841 cgccgttaat taacgaactg ctttgccagt taggtcaaca ccccgacttc gagaagtggc
     6901 aacaaaaggg taaactcccg tctaccgttg tgagccagtt atgtcaacct ctcaaaactg
     6961 accctcgctt tgcaggtcag cccagccgtt tatatatgtc ggcaattcat attgtggact
     7021 acatctacaa gtcctggctg gctatacaga aacggcttca acagcagcta gatggaaaga
     7081 cgcgctggct agaaatgctc aatagcgatg ctgaattagt agaacttagt ggtgacactt
     7141 tagaggctat tcgtgtcaaa gctgctgaaa ttttggcaat agctatgcca gcatctgagt
     7201 cagatagcgc ttcacctaaa gggaaaaaag gtaaaaagga gaaaaaaccc tcatcttcta
     7261 gccctaagcg tagtttatcc aagacattat ttgacgctta ccaagaaacg gaagatatca
     7321 agagccgtag cgccatcagc tacctgttaa aaaatggctg caaacttact gacaaagaag
     7381 aagattcaga aaaatttgct aaacgtcgtc gtcaagttga aatccaaatt caaaggctta
     7441 ccgaaaagtt aataagtcgg atgcctaaag gtcgagattt gaccaatgct aaatggttgg
     7501 agacactctt gactgctaca accactgttg ctgaagacaa cgcccaagcc aaacgctggc
     7561 aggatattct gttaactcga tcaagttctc tcccattccc ccttgttttt gaaaccaacg
     7621 aggatatggt ttggtcaaag aatcaaaagg gtaggctgtg tgttcacttc aatggcttaa
     7681 gcgatttaat ttttgaggtg tactgcggca atcgtcaact tcactggttt caacgcttcc
     7741 tagaagacca acagactaaa cgcaaaagca aaaatcagca ttctagcggc ttgttcacac
     7801 tcagaaatgg tcatctagtt tggcttgaag gtgagggtaa aggggaacct tggaatcttc
     7861 accacttgac cctttactgc tgtgttgaca atcgcttgtg gacagaggag ggaacagaaa
     7921 tcgttcgcca agagaaagca gatgaaatta ctaaattcat cacaaacatg aagaagaaaa
     7981 gcgatctaag cgatacacag caagctttga ttcaacgtaa acaatcaaca cttactcgaa
     8041 taaacaattc ctttgagcgt cctagccaac ccctttatca aggtcaatca cacattttgg
     8101 ttggagtaag cctgggacta gaaaaacctg ccacagtagc agtagtagat gcgatcgcca
     8161 acaaagtctt ggcttaccgg agtattaaac aattacttgg cgacaattac gaactgctaa
     8221 atcgccagag acgacaacag cagtacctat ctcacgaacg ccacaaagca caaaaaaact
     8281 tctctcccaa tcaatttgga gcatctgagt tagggcaaca tatagacaga ttattagcta
     8341 aagcaattgt agcgttagcg agaacctaca aagctggcag tattgtcttg cccaagttag
     8401 gggatatgcg ggaggttgtc caaagtgaaa ttcaagctat agcagaacaa aaatttcccg
     8461 gttatattga aggtcagcaa aaatatgcca aacagtaccg ggttaatgtt catcggtgga
     8521 gctacggcag attaattcaa agcattcaaa gtaaagcagc tcaaacagga attgtgattg
     8581 aggagggaaa acaacctatt cgaggtagtc cccacgacaa agcaaaggaa ttagcacttt
     8641 ctgcttacaa tctccgccta actaggcgaa gttaagtaag gatctccagg catcaaataa
     8701 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     8761 ctctctacgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8821 gcgcggggag aggcggtttg cgtattgggc gcatgcataa aaactgttgt aattcattaa
     8881 gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc gccagcggca
     8941 tcagcacctt gtcgccttgc gtataatatt tgcccatggg ggtgggcgaa gaactccagc
     9001 atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc
     9061 aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct
     9121 tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga
     9181 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc
     9241 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt
     9301 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga
     9361 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg
     9421 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat
     9481 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt
     9541 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca
     9601 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt
     9661 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag
     9721 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg
     9781 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca
     9841 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca
     9901 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc
     9961 atcctgtctc ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag
    10021 ccatccagtt tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt
    10081 ccggttcgct tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc
    10141 aagctacctg ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga
    10201 cattcatccc aggtggcact tttcggggaa atgtgcgcgc ccgcgttcct gctggcgctg
    10261 ggcctgtttc tggcgctgga cttcccgctg ttccgtcagc agcttttcgc ccacggcctt
    10321 gatgatcgcg gcggccttgg cctgcatatc ccgattcaac ggccccaggg cgtccagaac
    10381 gggcttcagg cgctcccgaa ggtctcgggc cgtctcttgg gcttgatcgg ccttcttgcg
    10441 catctcacgc gctcctgcgg cggcctgtag ggcaggctca tacccctgcc gaaccgcttt
    10501 tgtcagccgg tcggccacgg cttccggcgt ctcaacgcgc tttgagattc ccagcttttc
    10561 ggccaatccc tgcggtgcat aggcgcgtgg ctcgaccgct tgcgggctga tggtgacgtg
    10621 gcccactggt ggccgctcca gggcctcgta gaacgcctga atgcgcgtgt gacgtgcctt
    10681 gctgccctcg atgccccgtt gcagccctag atcggccaca gcggccgcaa acgtggtctg
    10741 gtcgcgggtc atctgcgctt tgttgccgat gaactccttg gccgacagcc tgccgtcctg
    10801 cgtcagcggc accacgaacg cggtcatgtg cgggctggtt tcgtcacggt ggatgctggc
    10861 cgtcacgatg cgatccgccc cgtacttgtc cgccagccac ttgtgcgcct tctcgaagaa
    10921 cgccgcctgc tgttcttggc tggccgactt ccaccattcc gggctggccg tcatgacgta
    10981 ctcgaccgcc aacacagcgt ccttgcgccg cttctctggc agcaactcgc gcagtcggcc
    11041 catcgcttca tcggtgctgc tggccgccca gtgctcgttc tctggcgtcc tgctggcgtc
    11101 agcgttgggc gtctcgcgct cgcggtaggc gtgcttgaga ctggccgcca cgttgcccat
    11161 tttcgccagc ttcttgcatc gcatgatcgc gtatgccgcc atgcctgccc ctcccttttg
    11221 gtgtccaacc ggctcgacgg gggcagcgca aggcggtgcc tccggcgggc cactcaatgc
    11281 ttgagtatac tcactagact ttgcttcgca aagtcgtgac cgcctacggc ggctgcggcg
    11341 ccctacgggc ttgctctccg ggcttcgccc tgcgcggtcg ctgcgctccc ttgccagccc
    11401 gtggatatgt ggacgatggc cgcgagcggc caccggctgg ctcgcttcgc tcggcccgtg
    11461 gacaaccctg ctggacaagc tgatggacag gctgcgcctg cccacgagct tgaccacagg
    11521 gattgcccac cggctaccca gccttcgacc acatacccac cggctccaac tgcgcggcct
    11581 gcggccttgc cccatcaatt tttttaattt tctctgggga aaagcctccg gcctgcggcc
    11641 tgcgcgcttc gcttgccggt tggacaccaa gtggaaggcg ggtcaaggct cgcgcagcga
    11701 ccgcgcagcg gcttggcctt gacgcgcctg gaacgaccca agcctatgcg ag
//
