LOCUS       sequence-385685-        9139 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral construct of doxycycline inducible VEGFA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Yuan S, Stewart KS, Yang Y, Abdusselamoglu MD, Parigi SM, Feinberg 
            TY, Tumaneng K, Yang H, Levorse JM, Polak L, Ng D, Fuchs E
  TITLE     Ras drives malignancy through stem cell crosstalk with the 
            microenvironment.
  JOURNAL   Nature. 2022 Nov 30. doi: 10.1038/s41586-022-05475-6.
  PUBMED    36450983
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Nov 30. doi: 10.1038/s41586-022-05475-6."
COMMENT     Sequence Label: TRE-VEGFA EF1a-rtTA3
FEATURES             Location/Qualifiers
     source          1..9139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3687..4054)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3812..3830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3848..3866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3884..3902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3920..3938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3956..3974
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3992..4010
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4028..4046
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        4114..4325
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             4347..5093
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5107..5695
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5160..5180)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5578..5589)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5903..6536
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6664..6680)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6664..6680)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6677..6699)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6688..6704
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6712..6718,6719..6736,6737..6742))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6757..6778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6895..6912)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7066..7654)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7146..7165)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7825..8616,8617..8685))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8448..8467
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8686..8790)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8838..8972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc ctcaccgcct tggcttgtca
     2521 catctgcaag tacgttcgtt taactcaagc tgcctcgcct tgcaacgcga gtctgtgttt
     2581 ttgcaggaac atttacacgt ctgcggatct tggacaaaca aatgctttct ccgctctgaa
     2641 caaggctcac agtgaacgct ccaggattta aaccgggatt tcttgcgctt tcgtttttga
     2701 ccctttccct ttcctcgaac tgattttttt tctggctttg ttctgtcttt ctttggtctg
     2761 cattcacatc tgctgtgctg taggaagctc atctctccta tgtgctggct ttggtgaggt
     2821 ttgatccgca tgatctgcat ggtgatgttg ctctctgacg tgggcacgca ctccagggct
     2881 tcatcgttac agcagcctgc acagcgcatc agcggcacac aggacggctt gaagatgtac
     2941 tctatctcgt cggggtactc ctggaagatg tccaccaggg tctcaatcgg acggcagtag
     3001 cttcgctggt agacatccat gaacttgatc acttcatggg acttctgctc tccttctgtc
     3061 gtgggtgcag cctgggacca cttggcatgg tggaggtaca gcagtaaagc cagggtccag
     3121 tgcacccaag agagcagaaa gttcatggtt tcggaggccc gtccggggcc ggcgcggctc
     3181 gcgctccctc tccggctcgg actgcgaggc agcccgctct cttgcgcgcc ttggcgggct
     3241 cctctccctt ctggaaccga ggcccgggcc ggggcctggg gcgcgcgcgc ggctggagca
     3301 ctgtctgcgc acacggccgc ctcacccgtc catgagctcg gctcctgaga gccgagcgcc
     3361 cactgcggcc ccctctcctc ttccttctct tcctcccctc tcttctcggg ccccggcttc
     3421 tccaggcccg agctagcact tctcccagct ccgatcggtt tgtctcctgc ggcgcagacc
     3481 acggctacta cggagcgaga agagcccaga agttggacga aaagtttccg tgcaatcccg
     3541 cgagccccga ccccctccac cccgcctccg ggcgcgggct tcggttcctc gcggctcgcc
     3601 gccgcgtcca ccgtcggcag cccgccggcg aggaggtggg cgctggggct gggggcggtg
     3661 tctgtctgtc tgtccgtcag gaattcttta cgagggtagg aagtggtacg gaaagttggt
     3721 ataagacaaa agtgttgtgg aattgaagtt tactcaaaaa atcagcactc ttttataggc
     3781 gccctggttt acataagcaa agcttatacg ttctctatca ctgataggga gtaaactgga
     3841 tatacgttct ctatcactga tagggagtaa actgtagata cgttctctat cactgatagg
     3901 gagtaaactg gtcatacgtt ctctatcact gatagggagt aaactcctta tacgttctct
     3961 atcactgata gggagtaaag tctgcatacg ttctctatca ctgataggga gtaaactctt
     4021 catacgttct ctatcactga tagggagtaa actcgaggtg ataattccac taggtcttga
     4081 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
     4141 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
     4201 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
     4261 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
     4321 cacaggatcg atcacgtacg gccaccatgt ctagactgga caagagcaaa gtcataaact
     4381 ctgctctgga attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc
     4441 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     4501 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccactcctgc cccctggaag
     4561 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     4621 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     4681 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     4741 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     4801 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgaaac
     4861 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     4921 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     4981 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     5041 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taaacgcgtc
     5101 tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     5161 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     5221 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     5281 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     5341 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     5401 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     5461 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     5521 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     5581 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     5641 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     5701 attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag
     5761 ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggtttttcca
     5821 gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     5881 tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc
     5941 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
     6001 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
     6061 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
     6121 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
     6181 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
     6241 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
     6301 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
     6361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     6541 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     6601 tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc
     6661 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     6721 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     6781 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     6841 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     6901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     7021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     7141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     7201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     7261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     7321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     7381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     7441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     7501 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     7561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     7621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     7681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     7741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     7921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     7981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     8101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     8161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     8221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     8281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     8341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     8401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     8461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     8521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     8581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     8641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     8701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     8761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     8821 cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8881 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8941 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     9001 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     9061 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     9121 gtactacaaa cttagtagt
//
