LOCUS       sequence-385946-       16266 bp    DNA     circular SYN 14-APR-2025
DEFINITION  pHM1 derived plasmid containing the Gateway cassette attR3-attR4 and
            GFP gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16266)
  AUTHORS   Li C, Wang L, Cseke LJ, Vasconcelos F, Huguet-Tapia JC, Gassmann W, 
            Pauwels L, White FF, Dong H, Yang B
  TITLE     Efficient CRISPR-Cas9 based cytosine base editors for 
            phytopathogenic bacteria.
  JOURNAL   Commun Biol. 2023 Jan 17;6(1):56. doi: 10.1038/s42003-023-04451-8.
  PUBMED    36646768
REFERENCE   2  (bases 1 to 16266)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2023-01-17"; volume: "6(1):56. doi"; pages: " 
            10.1038/s42003-023-04451-8"
COMMENT     Sequence Label: pHMattR3-attR4-GFP
FEATURES             Location/Qualifiers
     source          1..16266
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(96..684)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(176..195)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     745..767
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     764..780
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     764..780
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    800..923
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(964..1269)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(1066..1085)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1589..2269)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     2184..2203
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2323..2329,2330..2347,2348..2353))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(2378..2501)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2509..2526)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2509..2525)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2518..2540)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     RBS             2702..2713
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2720..3433
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      3445..3516
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3532..3559
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     oriT            6920..7029
                     /note="incP origin of transfer"
     primer_bind     complement(8094..8113)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(8178..8197)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(8911..9702)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      13761..14196
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             complement(14233..15204)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein of plasmid pSa"
                     /label=RepA
                     /translation="MPKNNKAPGHRINEIIKTSLALEMEDAREAGLVGYMARCLVQATM
                     PHTDPKTSYFERTNGIVTLSIMGKPSIGLPYGSMPRTLLAWICTEAVRTKDPVLNLGRS
                     QSEFLQRLGMHTDGRYTATLRNQAQRLFSSMISLAGEQGNDFGIENVVIAKRAFLFWNP
                     KRPEDRALWDSTLTLTGDFFEEVTRSPVPIRIDYLHALRQSPLAMDIYTWLTYRVFLLR
                     AKGRPFVQIPWVALQAQFGSSYGSRARNSPELDDKARERAERAALASFKYNFKKRLREV
                     LIVYPEASDCIEDDGECLRIKSTRLHVTRAPGKGARIGPPPT"
ORIGIN
        1 aacgtcagaa gccgactgca ctatagcagc ggaggggttg gagcaaaagg ccagcaaaag
       61 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      121 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      181 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      241 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      301 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      361 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      421 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      481 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      541 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      601 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      661 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacgaa ttcattggta
      721 cccggggatc ctctagaggg cccgagcgga taacaatttc acacaggaaa cagctatgac
      781 catgattacg ccaagctatc aactttgtat agaaaagttg aacgagaaac gtaaaatgat
      841 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      901 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
      961 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
     1021 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
     1081 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg ggactttatc
     1141 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
     1201 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
     1261 aaactgcatt tcaccaggcc ctgttctcgt cggcaaaaga gccgttcatt tcaataaacc
     1321 gggcgacctc agccatccct tcttgatttt ccgctttcca gcgttcggca cgcagacgac
     1381 gggcttcatt ctgcatggtt gtgcttaccg aaccggagat actgacatca tatatgcctt
     1441 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
     1501 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     1561 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
     1621 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     1681 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     1741 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     1801 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     1861 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     1921 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
     1981 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
     2041 ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
     2101 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
     2161 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
     2221 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
     2281 tagctcctga aaatctcgac ggatcctaac tcaaaatcca cacattatac gagccggaag
     2341 cataaagtgt aaagcctggg gtgcctaatg cggccgccat agtgactgga tatgttgtgt
     2401 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
     2461 atcattttac gtttctcgtt caactttatt atacatagtt gataattcac tggccgtcgt
     2521 tttacaacgt cgtgactggg aaaacgagct caagcttgtg aaacgtgatt tcatgcgtca
     2581 ttttgaatat tttgtaaatc ttatttaata atgtgtgcgg caattcacat ttaatttatg
     2641 aatgttttct taacatcgcg gcaactcaag aaacggcagg ttcggatctt agctactaga
     2701 gaaagaggag aaatactaga tgcgtaaagg cgaagagctg ttcactggtg tcgtccctat
     2761 tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg gcgagggtga
     2821 aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg gtaaactgcc
     2881 ggttccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct ttgctcgtta
     2941 tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag gctatgtgca
     3001 ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg aagtgaaatt
     3061 tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta aagaggacgg
     3121 caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgttt acatcaccgc
     3181 cgataaacaa aaaaatggca ttaaagcgaa ttttaaaatt cgccacaacg tggaggatgg
     3241 cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg gtcctgttct
     3301 gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc cgaacgagaa
     3361 acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc atggtatgga
     3421 tgaactgtac aaatgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     3481 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     3541 tcgggtgggc ctttctgcgt ttataagctt accaggatcg ggggcggcct cccggatcct
     3601 ggtacgccca tcgctgccga cctggcagcg tccagcaccg tgatgtggga acaagatgcg
     3661 gcccccttcg caggggcagc ggatgatttc ccggcattca acgaagagga gctcgcatgg
     3721 ttgatggagc tattgcctca gtcaggctca gtcggaggga cgatctgagg ggcggcaggg
     3781 attcgagtaa gaaacctcta ctgacagcaa gttagctcac ttttggctgt gttttacacg
     3841 aatccctgcc gaccctcttc tacaatccca accgccgcca cggttcaact ggcgacctgt
     3901 cccctgtagc gtttgaatgg cgctacgcgc agcgagggtc ttgagtgact accgaagcct
     3961 gggcatagca gtttgccccg catcaacttc gcgcattgct gggcgaacac ccaggcagcc
     4021 tgatcgacgc tgcagcagac cgcgtggagg gatcccgggc cctcgacgga atgttcaatc
     4081 ctgtagcgca tttgctccgt catcctttgg cctccaaata cagtgcccat gcttcctcta
     4141 taatgacgcc ttgcacgacg ccacgccgca tagcttcctc tgcgatttct tcggaaacgt
     4201 gcggcagcac tttggcgtgc acctggccgg tgcgctcgct cttcttgcga ccgggctttc
     4261 cgcgcggcga ccgatcaacg aaccccctcg cctcccctac tgcgtcagcc ttggcgatgg
     4321 ccgcttcgct ggccggcttc gcggtccgtt tcagcgaccc tagatctagt ttgaaatcat
     4381 tcatcgcagt gcctccgtta gcttcttaaa tattgccatg gcgaaggcct cggcgttttc
     4441 gattgccgcc gccatattgc cttgcttcga tttcattgaa tggagattgc cgccgaactg
     4501 aaaaagcgcg gaatatgccg tccgctctac aagtggcggt tcgatgattt ctactccgtt
     4561 ttcgatgagt gatgccttga tgccggtgta ttcatgtgaa cggatcgcgg ggctaaccat
     4621 ggtgaacacg accgcatgag ctattttccg gtctattgct tcttcttctt ctgcaatcag
     4681 ctgtagcgac tgcgcgccta tggttgcgtc cagtgccttg gggcgcattg gaattagcac
     4741 caagtctgct tgagatattg ctctagagac catgcgcgac gctacacctt caaggtcaac
     4801 aatgaccacg gctccatcaa cgtcatgctg cttgatcgtc ttgacgatgc tggattcggt
     4861 tacgtcgctt agagctgtga tattctccgg gacttccccc gcgtttgccc aaatagtcaa
     4921 tgaccggtta gggtcgcaat ccaacatggt gacggggaca cccttatgcg caagctcggt
     4981 tcccagcacc acggcggtgg tggatttccc tgccccgccc tttgatgaag ccactacgac
     5041 aacaggcata tgtcattctc cggttaaatc gataatctgg tttattcaaa tacccggtaa
     5101 aagttgatat cggattaata attaaatccg gtaaatgaat tgaccggatt taaacacaca
     5161 cccggttttg attgcaaccg ggtatttaca caaatccgat aattacaatt accggataag
     5221 cgaaattacc cggtatttca atgaaccggg tatcgcatca aatcggatat gccgactggc
     5281 taaagtaaag aagcagttaa ttttcaatgc ggggaattgg tgtctcgcac acggcttcga
     5341 cggcgtttct ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac
     5401 cagcccggtg agcgtcggaa aggcgctgga agccccgtag cgacgcggag aggggcgaga
     5461 caagccaagg gcgcaggctc gatgcgcagc acgacatagc cggttctcgc aaggacgaga
     5521 atttccctgc ggtgcccctc aagtgtcaat gaaagtttcc aacgcgagcc attcgcgaga
     5581 gccttgagtc cacgctagat ccctccacgc acgttgtgat atgtagatga taatcattat
     5641 cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct cgcgggtttt
     5701 cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc ataacttaat
     5761 gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa agcgagcttt
     5821 ttgggatctc gtctaccgtg tacccactct cacggccgac gaaacgctta aaggaacaat
     5881 agccatacgt tcgcttctca cgctgcaacg ttatcaggca tggcggcagc tcgttgctga
     5941 atagcttctc attgaaaaaa tcgtaggctc tctgaagttc atcgtaggtt tcgggtgtag
     6001 gcaaattcac tgctaacctc acatatatgt attgtgcaat acacataatt tagatgatcg
     6061 gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc ggcgcgcgga
     6121 tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc ctggtccttc
     6181 agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa gctctcgggt
     6241 aacatcaagg cccgatctgc tcggccattc cgacgtctct acgacgatga tttacacgca
     6301 tgtgctgaaa gttggcggtg ccggagtgcg ctcaccgctt gatgcgctgc cgcccctcac
     6361 tagtgagagg tagggcagcg caagtcaatc ctggcggatt cactacccct gcgcgaaggc
     6421 catcggtgcc gcatcgaacg gccggttgcg gaaagtcctc cctgcgtccg ctgatggccg
     6481 gcagcagccc gtcgttgcct gatggatcag caagcagaat tttcatttgc ttaatatcat
     6541 cttccgaaag tgacggtttt cttcccccta cacggcccct tgcgcgagca gctgcgagtc
     6601 cagatcgtgg catcaccgaa ccgcgccgtg cgcgggtcgt cggtgagcca gagtttcagc
     6661 aggccgccca ggcggcccag gtcgccattg atgcgggcca gctcgcggac gtgctcatag
     6721 tccacgacgc ccgtgatttt gtagccctgg ccgacggcca gcaggtaggc cgacaggctc
     6781 atgccggccg ccgccgcctt ttcctcaatc gctcttcgtt cgtctggaag gcagtacacc
     6841 ttgataggtg ggctgccctt cctggttggc ttggtttcat cagccatccg cttgccctca
     6901 tctgttacgc cggcggtagc cggccagcct cgcagagcag gattcccgtt gagcaccgcc
     6961 aggtgcgaat aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct
     7021 atcctgcccg gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa
     7081 aatcctgtat atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg
     7141 aagcagggtt atgcagcgga aaagcgctgc ttccctgctg ttttgtggaa tatctaccga
     7201 ctggaaacag gcaaatgcag gaaattactg aactgagggg acaggcgaga gacgatgcca
     7261 aagagctaca ccgacgagct ggccgagtgg gttgaatccc gcgcggccaa gaagcgccgg
     7321 cgtgatgagg ctgcggttgc gttcctggcg gtgagggcgg atgtcgaggc ggcgttagcg
     7381 tccggctatg cgctcgtcac catttgggag cacatgcggg aaacggggaa ggtcaagttc
     7441 tcctacgaga cgttccgctc gcacgccagg cggcacatca aggccaagcc cgccgatgtg
     7501 cccgcaccgc aggccaaggc tgcggaaccc gcgccggcac ccaagacgcc ggagccacgg
     7561 cggccgaagc aggggggcaa ggctgaaaag ccggcccccg ctgcggcccc gaccggcttc
     7621 accttcaacc caacaccgga caaaaaggat ctatctcatc tgcgcaaggc agaacgtgaa
     7681 gacggccgcc ctggacctcg cccgcgagcg ccaggcgcac gaggccggcg cgcggacccg
     7741 cgccacggcc cacgagcgga cgccgcagca ggagcgctga ggtctgcctc gtgaagaagg
     7801 tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc
     7861 acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc
     7921 cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt
     7981 tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     8041 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     8101 tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat
     8161 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
     8221 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgatac atggcaaagg
     8281 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
     8341 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     8401 tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     8461 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     8521 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     8581 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     8641 gaaagaaatg cataagctag cttagtaaag ccctcgctag attttaatgc ggatgttgcg
     8701 attacttcgc caactattgc gataacaaga aaaagccagc ctttcatgat atatctccca
     8761 atttgtgtag ggcttattat gcacgcttaa aaataataaa agcagacttg acctgatagt
     8821 ttggctgtga gcaattatgt gcttagtgca tctaacgctt gagttaagcc gcgccgcgaa
     8881 gcggcgtcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg tgatctcgcc
     8941 tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc
     9001 ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg
     9061 ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta
     9121 ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga
     9181 gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc
     9241 aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag
     9301 caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt
     9361 gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc
     9421 gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc
     9481 cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt
     9541 caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc
     9601 gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc
     9661 tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta actttgtttt
     9721 agggcgactg ccctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
     9781 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtaccccaa aaaaacagtc
     9841 ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct
     9901 ggaccagttg cgtgagcgca tacgctactt gcattacagc ttacgaaccg aacaggctta
     9961 tgtccactgg gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg
    10021 cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc
    10081 cacgcatcgt caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga
    10141 tctgccctgg cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct
    10201 gaccccggat gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc
    10261 ccagcttctg tatggaacgg gcatgcggat cagtgagggt ttgcaactgc gggtcaagga
    10321 tctggatttc gatcacggca cgatcatcgt gcgggagggc aagggctcca aggatcgggc
    10381 cttgatgtta cccgagagct tggcacccag cctgcgcgag cagctgtcgc gtgcacgggc
    10441 atggtggctg aaggaccagg ccgagggccg cagcggcgtt gcgcttcccg acgcccttga
    10501 gcggaagtat ccgcgcgccg ggcattcctg gccgtggttc tgggtttttg cgcagcacac
    10561 gcattcgacc gatccacgga gcggtgtcgt gcgtcgccat cacatgtatg accagacctt
    10621 tcagcgcgcc ttcaaacgtg ccgtagaaca agcaggcatc acgaagcccg ccacaccgca
    10681 caccctccgc cactcgttcg cgacggcctt gctccgcagc ggttacgaca ttcgaaccgt
    10741 gcaggatctg ctcggccatt ccgacgtctc tacgacgatg atttacacgc atgtgctgaa
    10801 agttggcggt gccggagtgc gctcaccgct tgatgcgctg ccgcccctca ctagtgagag
    10861 gtagggcagc gcaagtcaat cctggcggat tcactacccc tgcgcgaagg ccatcggtgc
    10921 cgcatcgaac ggccggttgc ggaaagtcct ccctgcgtcc gctgatggcc ggcagcagcc
    10981 cgtcgttgcc tgatggatct tcctgcattg ctcacgcagc tttgaaacct gaatcggcgc
    11041 tgctacgccc acgtgttctg ccaacataac ggcggatgct tcatcacggg aaagcaccgg
    11101 aaacacggcg acctcatggg ccaaaagcca cattgaacgg gtcatgccat cagtgaaaca
    11161 caccgcgaaa tggcccagaa tcatggtcgg gcttacgtca gcaagcggaa cggggctgtc
    11221 gatgcccacg gcaaaatgcg cggcgctcga cggatatttc cggtctttag gcatgtcctg
    11281 cttcaacggg caggcttgag aatcctcgac gcctaacgcc aaccacgctc gatagaaagc
    11341 caagccattc acatacacaa tgaaatcgga ctgtgcggca tttgaagctg tcataaaggc
    11401 gtcaggatgg ttcggcacgg gcagccgcca aacaacctca ttccaagcat tcggcacgat
    11461 ttgaggcaat tcgaggggca acccgctcga ctgcgcaagc tctttatcaa gccgccgaat
    11521 ttccagccaa gcgcgacacc gccgccgtag caacatcagc atcgttagcg cccatgctct
    11581 cggcctgcgc cggttttttc catctgccgc tgtcgttgat gttgcgcggc ctgtgggttg
    11641 cggacgattc cggcgcgacc gtgcggataa ctgatttccg catggtcgtt catcttcact
    11701 ttctccgcgc ccgaaacagc ggcgcgttca tgcagaatga tcgtgcctgg gcggctatcg
    11761 tcggcctgaa tgacatagct attggtgcga ccgatcacac ggccccggta agtttcgccc
    11821 ggttctgcgt ggcggtgaaa ttcatctgcg cgggctttct ccctgcctac tgcctcgcgc
    11881 acctgttgcg cctcgatgat ggccctttct acgtcgcgct caagccggtt ggcgttggtg
    11941 ctcttagtgg cgtctggcgt ggtttctagc tctttgcggg tctcttgtag tccgctcttg
    12001 tagttctcaa gcaagggttc attgcctttg gcttctttcg gaatttcggc caattggcgg
    12061 cctgcctcga aagccagcag gcgcatggtt tccgcttcat tctggcgcga ctgctcttgc
    12121 agcttccggc gttcgtcttt ctcctgctcg cggcgcgtgt cggtcatggt ctttcctcca
    12181 tcgtgtcaag ctcgcttcgc gtgccatagt gctcgcgccg ttcaacggcg ttgccgtcct
    12241 ccactacgtt gcggcccttc gggtgactcg gcgctgtgcg ctatggcgat ctggaagtgt
    12301 cgggacgagg ggcgtcccgg cggtaaattc gacgtgcctg cggcgcgtcg aacaaggggc
    12361 gtgcccggtg tcagttcgtg gcatttttcg aggcgcgacg ccatttccaa ggctcctgag
    12421 cattcgggtc tgaccaaagg ccgagccgtt ggcggcgggc ctctttttca tactcgtaca
    12481 tctgcgcgtc ggttggtttg ccgtaataac ggtaagccca ggccatgcct tcttgaacca
    12541 tgatcgcatt gatgttggtg agttgtgttt ggccgccggg gtattgcaac ggcgcgtaaa
    12601 cgaccccaag agtgcggcca taccgatcaa cctctttttc ggtcacttga acctcttggc
    12661 gaaaggtcaa gtcggcgagc cgttggcgag cacgggagcc gaaggcttgg ccgctttccg
    12721 gtgcgtcaat atcggccaat ctcacgcgga tggtctgacg gttcaccaaa acgtcgatag
    12781 tgtcaccgtc aaggattcgg acgacttcac cccggaagtc ggcccaagcg ggcacactga
    12841 cgattaggac gacagcggcc gcgaccgcgc gaagggcggc aagggcgctt ttcattgttt
    12901 gcctcctgtt ttcaagacgg ctgtgagatt ggcgacctgc tctttgaggg cttccacctg
    12961 accttgcaga ctggcggcgc gctcgatggc ctccttggcc tgtttgcggg cctcgatagc
    13021 ctcgttatcg cgttgggtga gcttttccat gcagcggttt agttcttcgc cgctgcggcg
    13081 tttcacttcg gcaagctggt cggccagctt gtcgcgctcg cgttccatag gttcgagctg
    13141 attcactcgt tcgcggagct ggtcgttttc gcgggtgaag gtgtcggcta gttcgattgc
    13201 ttcggcaagc tgctggctga tggccgcttt gtcggcctcg atctgtttcc gatcttcgtc
    13261 aaaccgggcg ttggcgtgcg ccagggcgat agcccatagc gcattgccaa gctcggcaag
    13321 atgctcgttg actgcaaccg gcaatgggtc tgatgagggc agggtggcgg tcttgcggtt
    13381 tttccattca gccattgcat cggaaatggt tgtgaagcta ccgcttccga gtttcttgcg
    13441 cacggcggcc aaagtgggcc ggatgccttc ggcgtccagt tcgtcggctg ctcgccaaat
    13501 gtcttgttta gtgattgcca ttcttgcggg cctctgtact gtagtatgtt gtatgatact
    13561 acatactaca acaatttaac agagccatct tggaatctgg tgtctctgcg cctataattc
    13621 tggaacagct actttccgaa cgactcctgc gttgatcgga aatccagaag cccgagaggt
    13681 tgccgccttt cgggcttttt ctttttcaaa aaaaaaaatt tataaaacga tctgttgcgg
    13741 ccgccgggtt gtgggcaaag gcgctcgacg gtgggcaacc gcttgcggtt gtccacgggc
    13801 ggagccggtg cgcgtagcgc attgtccaca agccaagggc gaccaataat tgatatatat
    13861 attcataatt gaaaagctaa ttgaacatac tacttgctgt aactacttgc cggagcgagg
    13921 ggtgtttgca agctgttgat ctgaaagggc tattagcgtt ctcacgtgcc tttttgatta
    13981 gcgatttcac gtgaccttat tagcgatttc acgtactccg attagcgatt tcacgtaccc
    14041 tgattagcga tttcacgtgg atagtttttg gagcgggccg gaaagccccg tgaatcaagg
    14101 ctttgcgggg cattagcggt ttcacgtgga taactaccct ctatccacag gcttccgggg
    14161 ataaaaaagc ccgctcgacg gcgggctgtt ggatgggaag gcttgaccaa gccaagcgta
    14221 gcgttggcct ggtcaagtcg gaggggggcc gatgcgagcg cccttgccgg gtgcgcgggt
    14281 gacatgcagg cgtgtggatt tgatgcgcag gcattcgccg tcatcttcga tgcagtcgct
    14341 tgcctcggga tagacaatca acacttcgcg taggcgcttt ttgaagttgt atttgaagct
    14401 ggcgagtgct gcccgctctg cccgctctcg ggccttatcg tccagttcgg gcgagttgcg
    14461 tgcgcggctg ccataggatg agccgaattg cgcttgcagg gcgacccaag ggatttgcac
    14521 gaaggggcgg cccttggccc gcaacaggaa cacgcgatag gtcagccacg tgtaaatgtc
    14581 catcgcaagc ggagactgcc gcaaggcatg caggtagtcg attcggatag gaaccggtga
    14641 gcgggtgact tcctcgaaga aatcgcctgt gagggtgagg gtgctatccc atagcgcccg
    14701 atcttctggc cgcttgggat tccagaatag aaaagcgcgc ttggcaatga cgacgttctc
    14761 aatgccgaag tcattgcctt gctcgccggc aagcgaaatc atggatgaaa acaggcgttg
    14821 cgcctgattg cgaagggtgg ccgtgtaacg gccatcggtg tgcattccga gcctttgtag
    14881 aaattccgat tgcgaccggc caaggttcaa cacggggtct ttcgttcgca cggcctcggt
    14941 gcatatccaa gcaagcaagg tgcgcggcat agaaccgtag ggcaggccga tgctcggctt
    15001 gcccatgatc gacaaggtga cgatgccatt ggtgcgctca aagtagctgg tcttggggtc
    15061 ggtgtggggc atggtcgctt gcacaaggca acgggccatg tagccgacta agccagcttc
    15121 gcgggcatcc tccatttcga gcgcgaggct cgtcttgatg atctcgttga tacgatggcc
    15181 gggggctttg ttgttcttag gcatgttgtt ccctccccgg catggtgatg gttggtctag
    15241 tgtttgtggg tttgatgttc cggcgtttga tgaacaggcg caaggtgtga gggctgacgc
    15301 ctaacaactc ggctgcgcga ctttgcggca agccaaggtt cacgtatgcc tgtacttcat
    15361 caatacggct gtccagcttc aaggcgctcg atttgctgcc cttgggtcgc ccgagcgtct
    15421 tgccgcgctc tctggcgact tgtagcgcct cggtggtacg tgcctgaatg aaatgccgct
    15481 cgatctgtgc agccaagcca agcacggttg ccatgatgtc gctttgtagg ctgccgtcca
    15541 tgatgatctt ctgtttggtc acatggacga ttaggccgcg ctcgctcgcc gctttgagaa
    15601 tttccaaggc ggcgagggcg gaaccggcaa tgcgcgtaat ctccggcgtc agtagcacgt
    15661 cgccacgctc ggccttttcg atgattgctc cgagcttgcg cttgcgccag tcctttgctc
    15721 tgctggcaat ttcttcctcg atctgtagcg gcgcgaagcc tttggcgttc gcgtattcga
    15781 gcaaaccgta tttttggttt tccgggtctt ggccgtcacg cgaaacccgg agataggcat
    15841 agtattttgg catttgcagg gaaaacgtca gattcggtta aacatgcctc attctagcgc
    15901 agattaaata ggaattaaat accctgttgc ggtatagata aaacgttggt ttgttctgcc
    15961 ctatgagcgt acaaaaaagg ccgggtgagt ggcccggcct tcgtttaggt gctgaatagg
    16021 attggttctg gtgccagcct catgagaagc gcgtcataaa accacatgag ggccgacgca
    16081 ccaaggccga cgcctgcgac cgatagcatg atgtgggtct tattggccga gtccagccca
    16141 agccacatga tcggtagggt gatgagactg gcgaacgaag ccaagccgag aagaaagcgc
    16201 accgggccgc gcagccacca gagaatgagg aacaccagat gtcgttttca gaagacggct
    16261 gcactg
//
