LOCUS       sequence-386194-        2257 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A2 Proximal promoter sequence consisting of three times the target 
            sequence for the gRNA1 (GB1838) flanked by random sequences. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Moreno-Gimenez E, Selma S, Calvache C, Orzaez D
  TITLE     GB_SynP: A Modular dCas9-Regulated Synthetic Promoter Collection for
            Fine-Tuned Recombinant Gene Expression in Plants.
  JOURNAL   ACS Synth Biol. 2022 Sep 16;11(9):3037-3048. doi: 
            10.1021/acssynbio.2c00238. Epub 2022 Aug 31.
  PUBMED    36044643
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00238"; journalName: "ACS Synth Biol"; date: 
            "2022-09-16- 16"; volume: "11"; issue: "9"; pages: "3037-3048"
COMMENT     Sequence Label: GB3277
FEATURES             Location/Qualifiers
     source          1..2257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(445..502)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    711..732
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      735..778
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(782..800)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             962..1621
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1028..1047)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1642..1736
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1919..2257,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctgtggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcctgc ataacgcgaa gtaatctttt cggttttaaa gaaaaagggc agggtggtga
      481 caccttgccc ttttttgccg gactgcagcg gccgctacta gtcgcgatga gtggtctcag
      541 ggaaacgaac ggcctcggac tggttggtga gagaagatgg ctatagagtc gatccacatc
      601 aacgccagac tggttggtga gagaagaagg ttagcacgtg gccagcacct tagcgtgaag
      661 cggtcgtgac tggttggtga gagaagaggg agtcacgaga ccactcatcg ctctagaagc
      721 ggccgcgaat tccagaaatc atccttagcg aaagctaagg atttttttta tctgaaattc
      781 tgcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
      841 tcaggtggca ctcgagttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
      901 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      961 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     1021 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     1081 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     1141 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
     1201 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     1261 aacgttttca tcactctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1321 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1381 gaatatgttt ttcgtatcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1441 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1501 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1561 tgtcggcaga atgcttaatg aattacaaca gtactgcgac gagtggcagg gcggggcgta
     1621 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     1681 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     1741 gcctcgagct gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1801 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1861 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1921 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1981 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2041 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2101 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2161 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2221 cgcagcggtc gggctgaacg gggggttcgt gcacaca
//
