LOCUS       sequence-386429-        9413 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses APP695 with two fluorescent proteins at the ends to follow
            processing in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Capitini C, Bigi A, Parenti N, Emanuele M, Bianchi N, Cascella R, 
            Cecchi C, Maggi L, Annunziato F, Pavone FS, Calamai M
  TITLE     APP and Bace1: Differential effect of cholesterol enrichment on 
            processing and plasma membrane mobility.
  JOURNAL   iScience. 2023 Apr 11;26(5):106611. doi: 10.1016/j.isci.2023.106611.
            eCollection 2023 May 19.
  PUBMED    37128606
REFERENCE   2  (bases 1 to 9413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-04-11"; volume: "26(5):106611. doi"; pages: " 
            10.1016/j.isci.2023.106611. eCollection 2023 May 19"
COMMENT     Sequence Label: mCherry-APP-mGFP
FEATURES             Location/Qualifiers
     source          1..9413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..173
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     165..182
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     166..182
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        343..722
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        723..926
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     877..897
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     952..971
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        952..970
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1086..1793
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1231..1249)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1499..1518
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(2091..2102)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             2582..2602
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             3870..4583
                     /codon_start=1
                     /product="monomeric green fluorescent protein, also known 
                     as mTagGFP"
                     /label=TagGFP2
                     /note="mammalian codon-optimized"
                     /translation="MSGGEELFAGIVPVLIELDGDVHGHKFSVRGEGEGDADYGKLEIK
                     FICTTGKLPVPWPTLVTTLCYGIQCFARYPEHMKMNDFFKSAMPEGYIQERTIQFQDDG
                     KYKTRGEVKFEGDTLVNRIELKGKDFKEDGNILGHKLEYSFNSHNVYIRPDKANNGLEA
                     NFKTRHNIEGGGVQLADHYQTNVPLGDGPVLIPINHYLSTQTKISKDRNEARDHMVLLE
                     SFSACCHTHGMDELYR"
     primer_bind     complement(4618..4634)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4645..5267
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4844..4863)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5291..5311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5296..5625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5476..5611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5692..6486
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5746..5765)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6356..6375
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6660..6781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6697..6716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6751..6770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6830..6846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6843..6865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6854..6870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6878..6884,6885..6902,6903..6908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6923..6944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7061..7078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7232..7820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7312..7331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7991..8782,8783..8851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8614..8633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8852..8956)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8983..9411
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9070..9089)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9280..9301
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
       61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
      121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      181 gtgccaagct gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt
      241 atatagcata aatcaatatt ggctattggc cattgcatac gttgtatcta tatcataata
      301 tgtacattta tattggctca tgtccaatat gaccgccatg ttgacattga ttattgacta
      361 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      421 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      481 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      541 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      601 gtccgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      661 tgaccttacg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      721 tggtgatgcg gttttggcag tacaccaatg ggcgtggata gcggtttgac tcacggggat
      781 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      841 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      901 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagaatttt gtaatacgac
      961 tcactatagg gcggccggga attcgtcgac tggatccggt accgaggaga tctgccgccg
     1021 cgatcgccat gctgcccggt ttggcactgc tcctgctggc cgcctggacg gctcgggcgg
     1081 ctagcatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1141 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1201 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1261 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1321 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1381 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1441 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1501 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     1561 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     1621 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     1681 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     1741 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagagtcgtg
     1801 gaacaggcgc gcctctggag gtacccactg atggtaatgc tggcctgctg gctgaacccc
     1861 agattgccat gttctgtggc agactgaaca tgcacatgaa tgtccagaat gggaagtggg
     1921 attcagatcc atcagggacc aaaacctgca ttgataccaa ggaaggcatc ctgcagtatt
     1981 gccaagaagt ctaccctgaa ctgcagatca ccaatgtggt agaagccaac caaccagtga
     2041 ccatccagaa ctggtgcaag cggggccgca agcagtgcaa gacccatccc cactttgtga
     2101 ttccctaccg ctgcttagtt ggtgagtttg taagtgatgc ccttctcgtt cctgacaagt
     2161 gcaaattctt acaccaggag aggatggatg tttgcgaaac tcatcttcac tggcacaccg
     2221 tcgccaaaga gacatgcagt gagaagagta ccaacttgca tgactacggc atgttgctgc
     2281 cctgcggaat tgacaagttc cgaggggtag agtttgtgtg ttgcccactg gctgaagaaa
     2341 gtgacaatgt ggattctgct gatgcggagg aggatgactc ggatgtctgg tggggcggag
     2401 cagacacaga ctatgcagat gggagtgaag acaaagtagt agaagtagca gaggaggaag
     2461 aagtggctga ggtggaagaa gaagaagccg atgatgacga ggacgatgag gatggtgatg
     2521 aggtagagga agaggctgag gaaccctacg aagaagccac agagagaacc accagcattg
     2581 ccaccaccac caccaccacc acagagtctg tggaagaggt ggttcgagtt cctacaacag
     2641 cagccagtac ccctgatgcc gttgacaagt atctcgagac acctggggat gagaatgaac
     2701 atgcccattt ccagaaagcc aaagagaggc ttgaggccaa gcaccgagag agaatgtccc
     2761 aggtcatgag agaatgggaa gaggcagaac gtcaagcaaa gaacttgcct aaagctgata
     2821 agaaggcagt tatccagcat ttccaggaga aagtggaatc tttggaacag gaagcagcca
     2881 acgagagaca gcagctggtg gagacacaca tggccagagt ggaagccatg ctcaatgacc
     2941 gccgccgcct ggccctggag aactacatca ccgctctgca ggctgttcct cctcggcctc
     3001 gtcacgtgtt caatatgcta aagaagtatg tccgcgcaga acagaaggac agacagcaca
     3061 ccctaaagca tttcgagcat gtgcgcatgg tggatcccaa gaaagccgct cagatccggt
     3121 cccaggttat gacacacctc cgtgtgattt atgagcgcat gaatcagtct ctctccctgc
     3181 tctacaacgt gcctgcagtg gccgaggaga ttcaggatga agttgatgag ctgcttcaga
     3241 aagagcaaaa ctattcagat gacgtcttgg ccaacatgat tagtgaacca aggatcagtt
     3301 acggaaacga tgctctcatg ccatctttga ccgaaacgaa aaccaccgtg gagctccttc
     3361 ccgtgaatgg agagttcagc ctggacgatc tccagccgtg gcattctttt ggggctgact
     3421 ctgtgccagc caacacagaa aacgaagttg agcctgttga tgcccgccct gctgccgacc
     3481 gaggactgac cactcgacca ggttctgggt tgacaaatat caagacggag gagatctctg
     3541 aagtgaagat ggatgcagaa ttccgacatg actcaggata tgaagttcat catcaaaaat
     3601 tggtgttctt tgcagaagat gtgggttcaa acaaaggtgc aatcattgga ctcatggtgg
     3661 gcggtgttgt catagcgaca gtgatcgtca tcaccttggt gatgctgaag aagaaacagt
     3721 acacatccat tcatcatggt gtggtggagg ttgacgccgc tgtcacccca gaggagcgcc
     3781 acctgtccaa gatgcagcag aacggctacg aaaatccaac ctacaagttc tttgagcaga
     3841 tgcagaacac gcgtacgcgg ccgctcgaga tgagcggggg cgaggagctg ttcgccggca
     3901 tcgtgcccgt gctgatcgag ctggacggcg acgtgcacgg ccacaagttc agcgtgcgcg
     3961 gcgagggcga gggcgacgcc gactacggca agctggagat caagttcatc tgcaccaccg
     4021 gcaagctgcc cgtgccctgg cccaccctgg tgaccaccct ctgctacggc atccagtgct
     4081 tcgcccgcta ccccgagcac atgaagatga acgacttctt caagagcgcc atgcccgagg
     4141 gctacatcca ggagcgcacc atccagttcc aggacgacgg caagtacaag acccgcggcg
     4201 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc aaggacttca
     4261 aggaggacgg caacatcctg ggccacaagc tggagtacag cttcaacagc cacaacgtgt
     4321 acatccgccc cgacaaggcc aacaacggcc tggaggctaa cttcaagacc cgccacaaca
     4381 tcgagggcgg cggcgtgcag ctggccgacc actaccagac caacgtgccc ctgggcgacg
     4441 gccccgtgct gatccccatc aaccactacc tgagcactca gaccaagatc agcaaggacc
     4501 gcaacgaggc ccgcgaccac atggtgctcc tggagtcctt cagcgcctgc tgccacaccc
     4561 acggcatgga cgagctgtac aggtccggac tcagataagt ttaaacggcc ggccgcggtc
     4621 atagctgttt cctgaacaga tcccgggtgg catccctgtg acccctcccc agtgcctctc
     4681 ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa attaagttgc
     4741 atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg ggtggtatgg
     4801 agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg ggaaccaagc
     4861 tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt tcaagcgatt
     4921 ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag gctcagctaa
     4981 tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt ctccaactcc
     5041 taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca ggcgtgaacc
     5101 actgctccct tccctgtcct tctgatttta aaataactat accagcagga ggacgtccag
     5161 acacagcata ggctacctgg ccatgcccaa ccggtgggac atttgagttg cttgcttggc
     5221 actgtcctct catgcgttgg gtccactcag tagatgcctg ttgaattggg tacgcggcca
     5281 gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     5341 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     5401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     5461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     5521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     5581 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     5641 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     5701 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     5761 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     5821 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5881 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5941 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     6001 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     6061 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     6121 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     6181 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     6241 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     6301 tctggattca tcgactgtgg ccggctgggt gtggccgacc gctatcagga catagcgttg
     6361 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     6421 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     6481 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     6541 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     6601 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     6661 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6721 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6781 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     6841 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6901 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6961 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     7021 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     7081 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     7141 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     7201 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     7261 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     7321 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     7381 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     7441 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     7501 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     7561 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     7621 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     7681 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     7741 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     7801 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     7861 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     7921 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     7981 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     8041 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     8101 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     8161 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     8221 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     8281 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     8341 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     8401 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     8461 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     8521 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     8581 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     8641 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     8701 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     8761 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     8821 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     8881 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     8941 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
     9001 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9061 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9121 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9181 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9241 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9301 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9361 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat ttt
//
