LOCUS       sequence-386578-        4575 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Donor Vector containing ZNF467 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR223-ZNF467
FEATURES             Location/Qualifiers
     source          1..4575
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1886..1985)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2002..2021)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2003..2021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2026..2042)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2026..2042)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2473..3264
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3357..3945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3846..3865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4099..4116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4275..4302)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4394..4480)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4529..4551
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4543..4560
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4544..4560
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagttggca tgagagagac cttggaggcc
      121 ctcagctccc tgggattctc tgtgggacag ccagagatgg ccccccaaag tgagcccagg
      181 gaaggatccc ataatgccca ggagcagatg tcctcttcta gggaagagag agcactgggg
      241 gtgtgctcag ggcacgaggc ccctacaccg gaggaaggtg cccacacaga acaagccgag
      301 gctccctgca gaggccaggc gtgctcagca cagaaggctc agcctgtggg tacctgccca
      361 ggagaggagt ggatgattcg gaaggtgaag gtggaggacg aagatcagga ggcagaagag
      421 gaggtcgaat ggccccagca tctatcgtta cttcccagcc cctttcccgc gcctgacctg
      481 gggcatctgg ctgccgcgta caaactggag ccaggggccc cgggggcact gagtgggctc
      541 gcgctgtctg ggtggggtcc gatgccggag aagccctacg gctgcgggga gtgtgagcgg
      601 cgcttccggg accagctgac gttgcgactg caccagcggc tgcaccgggg cgagggcccc
      661 tgcgcctgcc cggactgcgg ccgcagcttc acgcagcgcg cccacatgct actgcatcag
      721 cgcagccacc gcggcgagcg gcctttcccg tgctccgagt gcgacaagcg cttcagcaag
      781 aaggcccatc tgacccgcca cctgcgcacg cacacgggcg agcggcccta cccgtgcgcg
      841 gagtgcggca agcgcttcag ccagaagatc cacctgggct cgcaccaaaa gacccacacc
      901 ggcgagcggc ccttcccctg cacggaatgc gagaagcgct ttcgcaagaa gacgcacttg
      961 attcggcacc agcgcatcca tacgggcgag aggccctacc agtgcgcaca gtgcgcacgc
     1021 agcttcacgc acaagcagca cttggtgcgg caccaaaggg tgcaccagac ggccggcccg
     1081 gccaggccct ctcccgactc gtccgcttct cctcattcca ctgccccgtc cccgacccca
     1141 tcctttcccg ggccaaagcc tttcgcgtgc tccgactgcg gcttgagctt cggctggaaa
     1201 aagaacctcg ccacgcacca gtgtctgcac cgcagcgagg gtcgcccctt tgggtgcgat
     1261 gagtgcgcac tgggcgccac cgtggatgcc cccgccgcca agcccctggc cagcgcgcct
     1321 ggcggaccgg gctgcggccc aggatccgat cccgtggtgc cccagcgcgc cccctcgggc
     1381 gagcggtcct tcttctgccc ggactgcggg cgcggcttct cccatgggca gcacctggcg
     1441 cggcacccgc gcgtgcacac gggcgaacgg cccttcgcct gcacgcagtg tgaccgccgc
     1501 ttcggctcgc ggcctaatct ggtcgcccac tccagggccc acagcggcgc caggcctttc
     1561 gcctgcgctc agtgcggccg ccgcttcagc cgcaagtcgc acctgggccg ccaccaggcg
     1621 gtgcacactg gcagccgccc ccacgcctgc gccgtctgcg cccgcagctt cagctccaaa
     1681 accaacctag tccgccacca ggcgatccac acaggctccc gccccttctc ctgcccgcag
     1741 tgcggaaaga gcttcagccg caagacccac ctggtgcggc accagctcat tcacggcgaa
     1801 gccgcccacg cggccccgga cgccgccctt gcggccccag cctggtccgc tccccccgag
     1861 gtggcgccgc ccccgctctt cttctgccca actttcttgt acaaagttgg cattataaga
     1921 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     1981 atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc
     2041 tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     2101 tcatcatgcc tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag
     2161 gttgccgggt gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc
     2221 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     2281 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     2341 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     2401 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     2461 aagttaaaca ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     2521 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     2581 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     2641 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     2701 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     2761 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     2821 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     2881 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     2941 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     3001 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     3061 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     3121 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     3181 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     3241 atcaccaagg tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg
     3301 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     4021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     4081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     4141 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     4201 gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag
     4261 tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg
     4321 cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat
     4381 ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
     4441 gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact
     4501 ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt
     4561 cttaagctcg ggccc
//
