LOCUS       sequence-38744-e         291 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Stephan J, Stemmer V, Niederweis M
  TITLE     Consecutive gene deletions in Mycobacterium smegmatis using the 
            yeast FLP recombinase.
  JOURNAL   Gene. 2004 Dec 8;343(1):181-90.
  PUBMED    15563844
REFERENCE   2  (bases 1 to 291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "2004-12-8"; volume: "343(1)"; pages: "181-90"
COMMENT     Sequence Label: pMN252
FEATURES             Location/Qualifiers
     source          1..291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc a
//
