LOCUS       sequence-387860-       14169 bp    DNA     circular SYN 14-APR-2025
DEFINITION  T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14169)
  AUTHORS   Li Z, Zhong Z, Wu Z, Pausch P, Al-Shayeb B, Amerasekera J, Doudna 
            JA, Jacobsen SE
  TITLE     Genome editing in plants using the compact editor CasPhi.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jan 24;120(4):e2216822120. doi: 
            10.1073/pnas.2216822120. Epub 2023 Jan 18.
  PUBMED    36652483
REFERENCE   2  (bases 1 to 14169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-01-24"; volume: "120(4):e2216822120. 
            doi"; pages: " 10.1073/pnas.2216822120. Epub 2023 Jan 18"
COMMENT     Sequence Label: pC1300_pUB10_pco-nCASphi_E9t_MCS_version2
FEATURES             Location/Qualifiers
     source          1..14169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10..26)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(23..45)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     928..948
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2825..3619
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3706..4294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4195..4214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4448..4465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    4480..4620
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(4546..4565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4706..4728
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      4964..5158
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(5224..6297)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6726..7355)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    8656..8680
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     8868..8890
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8882..8899
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8883..8899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          11905..12093
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             13412..13432
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13439..13459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13478..13525
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata
     2821 ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     2881 cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     2941 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     3001 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     3061 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     3121 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     3181 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     3241 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     3301 ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     3361 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     3421 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     3481 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     3541 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     3601 actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc
     3661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     3841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     4441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     4501 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     4561 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     4621 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     4681 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     4741 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     4801 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     4861 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     4921 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     4981 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     5041 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     5101 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     5161 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     5221 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     5281 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     5341 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     5401 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     5461 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     5521 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
     5581 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
     5641 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
     5701 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
     5761 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
     5821 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
     5881 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
     5941 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
     6001 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
     6061 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
     6121 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
     6181 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
     6241 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
     6301 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt
     6361 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
     6421 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
     6481 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
     6541 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
     6601 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
     6661 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
     6721 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
     6781 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
     6841 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
     6901 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
     6961 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
     7021 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
     7081 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
     7141 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
     7201 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
     7261 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
     7321 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
     7381 ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac
     7441 atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg
     7501 ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg
     7561 cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga
     7621 aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt
     7681 ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg
     7741 cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg
     7801 atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc
     7861 gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg
     7921 ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc
     7981 ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca
     8041 cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag
     8101 taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg
     8161 gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt
     8221 ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc
     8281 ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat
     8341 ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac
     8401 aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga
     8461 catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg
     8521 gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac
     8581 aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac
     8641 gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg
     8701 aaggcgggaa acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct
     8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
     8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
     8881 ttgtaaaacg acggccagtg ccaagcttgc atgcctgcag gtcgactcta gatcactagt
     8941 atcctaggaa ggtaccagtc tagctcaaca gagcttttaa cccaaattgg tacaatagaa
     9001 tacaacttta gatcataatt ctcaaaagaa agagattcct tagctattct atctgccact
     9061 ccatttcctt ctcggcttgt atgcacaagc ataaaatcct caaacttgct aagtagatac
     9121 tttatgtctt ggataattgg attgagactt gacaagcata actttcatgt aaccaaagac
     9181 acaagttgct gagaatccac ctcaaaaatg atcttcctat aattgaatcg ggataatgac
     9241 agcacagccc atctaagagc ctccacttct acttccagca cgcttcttac ttttaccaca
     9301 gctcttgcac ctaaccataa caccttccct gtatgatcgc gaagcaccca ccctaagcca
     9361 cattttaatc cttctgttgg ccatgcccca tcaaagttgc acttaaccca agattgtggt
     9421 ggagcttccc atgtttctcg tctgtcccga cggtgttgtg gttggtgctt tccttacatt
     9481 ctgagcctct ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc
     9541 attggttcca aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc
     9601 aagtatccaa gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg
     9661 ttgaactcta tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc
     9721 atagcgaact tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa
     9781 aaaatattat tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa
     9841 gatccattga tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt
     9901 ttttaacgag acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa
     9961 tcatacaaaa atatccaata acactaaaaa attaaaagaa atggataatt tcacaatatg
    10021 ttatacgata aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat
    10081 tagataaatg gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa
    10141 atacgaaata cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc
    10201 caccgtatat ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt
    10261 aaatctcaac ggctggatct tatgacgacc gttagaaatt gtggttgtcg acgagtcagt
    10321 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
    10381 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
    10441 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
    10501 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
    10561 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
    10621 ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt
    10681 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
    10741 cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
    10801 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt
    10861 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtcgattaa
    10921 tctgagtttt tctgattaac agatgccgaa gcccgccgtc gaatcagagt tttccaaagt
    10981 cctcaagaaa cactttcctg gggagcgttt taggtctagc tatatgaaga gggggggtaa
    11041 aattctggca gcacaaggcg aggaagctgt agtggcgtac ttgcagggaa agagtgagga
    11101 ggaaccgccg aattttcagc cgccggcgaa gtgccacgtg gtcaccaaaa gcagggattt
    11161 cgcagaatgg cccataatga aagcctctga agccatacag aggtacatct atgcgctcag
    11221 cactacagag cgagctgcct gcaaaccggg taagagctca gaaagtcacg cggcctggtt
    11281 cgcggctaca ggggtgagca atcacggcta ttctcatgta caaggtctta acctgatctt
    11341 tgaccacacg ctaggacgat acgatggcgt tttaaagaaa gtacagcttc gaaatgagaa
    11401 ggcccgagct agattggctg caataaacgc cgctcgagct gcagctggat tgccggaaat
    11461 caaggccgag gaggaggagg tagctacaaa tgaaacaggt catctactac agccgccagg
    11521 cataaaccca tcattctacg tctaccagac catatctccg caggcttacc gaccaaggga
    11581 cgaaatagtg ttaccacccg agtacgccgg ttacgtcagg gatccgaacg ctccgattcc
    11641 actgggcgtg gtcaggaacc gttgtgacat acagaagggt tgcccgggat atatacccga
    11701 gtggcagagg gaagctggta cggcaattag tcccaagaca ggaaaagcag tgacggttcc
    11761 aggacttagc ccgaagaaga ataaacgtat gcgtaggtac tggaggtcag aaaaggagaa
    11821 ggctcaagat gcacttctcg taactgtaag gataggtacc gattgggtag tcatagacgt
    11881 aagaggatta ctaagaaacg ccaggtaagt ttctgcttct acctttgata tatatataat
    11941 aattatcatt aattagtagt aatataatat ttcaaatatt tttttcaaaa taaaagaatg
    12001 tagtatatag caattgcttt tctgtagttt ataagtgtgt atattttaat ttataacttt
    12061 tctaatatat gaccaaaatt tgttgatgtg caggtggagg actattgctc ccaaggacat
    12121 aagtttaaat gcacttctag atttatttac cggtgatcca gtcatcgatg tcagacgaaa
    12181 catcgtgacc ttcacctata ccttggacgc ttgcggaact tatgctagaa aatggactct
    12241 caagggaaaa cagacaaaag caaccttaga taaactgaca gcgacacaaa ctgtggcctt
    12301 agttgctata gatctgggac aaacaaaccc aattagcgcg ggtatcagtc gtgtcacaca
    12361 ggagaacggg gccctccagt gcgaaccgct tgatcgtttt acattgcctg atgacctttt
    12421 gaaagatatt tctgcgtacc gaattgcatg ggaccgtaac gaggaggaac tcagggccag
    12481 atccgttgag gcactcccag aggcacaaca agcagaggtc agagctctgg acggggtctc
    12541 caaagagacc gcgcgtacac agttgtgcgc ggacttcggt ctggacccaa agcgactacc
    12601 gtgggataaa atgagtagca ataccacgtt cataagcgag gcgctccttt ccaacagcgt
    12661 atcccgtgac caagtattct ttaccccggc cccaaagaaa ggagccaaga agaaagcacc
    12721 ggtggaagtg atgcgaaaag acaggacatg ggcgcgagcg tacaaaccac gactctcagt
    12781 agaagcacaa aagttgaaaa atgaggctct ttgggctttg aagcgtacct ctccagaata
    12841 tctaaagttg tcacgacgta aagaagaatt gtgtaggagg tccattaatt acgtgataga
    12901 aaaaactaga aggcgaaccc aatgccaaat tgtgatcccc gttatagaag atctaaatgt
    12961 ccgatttttc cacgggtctg gcaaacgact cccgggctgg gataacttct tcacggcaaa
    13021 gaaggaaaac cgatggttta tccaagggct acataaggcg ttttctgact taaggaccca
    13081 ccgatccttc tacgtgttcg aggtgcgtcc agagagaaca tcaatcacct gtccgaaatg
    13141 cgggcactgt gaagtgggga acagggatgg agaagccttt cagtgtctca gttgcggtaa
    13201 gacatgcaac gcagatctgg acgtagcgac acataacttg actcaagtgg cgctcaccgg
    13261 caaaacaatg ccgaagagag aggagcctag agatgcacaa gggacagccc ccgcgagaaa
    13321 gacgaagaaa gctagcaagt caaaggcacc cccggctgaa cgtgaggatc agactcccgc
    13381 tcaagagccg tcccaaacgt ccggatccgg accgaagaaa aagcgaaagg tagaggatcc
    13441 taaaaagaag cgtaaagtct ccttgggttc tggctccgac tataaggatg acgatgacaa
    13501 agactataag gatgacgacg acaagtgaag agctttcgtt cgtatcatcg gtttcgacaa
    13561 cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct actgagtttg
    13621 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
    13681 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
    13741 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
    13801 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
    13861 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca cttgtagttg
    13921 taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa agctgcaaat
    13981 gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt cctttatgta
    14041 attttccaga atccttgtca gattctaatc attgctttat aattatagtt atactcatgg
    14101 atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca attgattgac
    14161 aacgaattc
//
