LOCUS       sequence-388073-        9232 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Plasmid expressing an antigen targeting the Plasmid expressing an 
            antigen targeting Neurofascin-155.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Collinson JM, Marshall D, Gillespie CS, Brophy PJ
  TITLE     Transient expression of neurofascin by oligodendrocytes at the onset
            of myelinogenesis: implications for mechanisms of axon-glial 
            interaction.
  JOURNAL   Glia. 1998 May;23(1):11-23
  PUBMED    9562181
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Glia."; 
            date: "1998-05"; volume: "23(1)"; pages: "11-2"
COMMENT     Sequence Label: NF-155 pcDNA3
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     238..258
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(267..695)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(377..398)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     589..608
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    741..965
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     954..971
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        991..1009
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     991..1008
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      4816..4825
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4912..4931)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4913..4931)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4976..5179)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5005..5025)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5180..5559
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5731..5750
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5825..5929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5930..5998,5999..6790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6148..6167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6961..7549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7450..7469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7703..7720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7837..7858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7873..7878,7879..7896,7897..7903)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7911..7927
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7916..7938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7935..7951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7935..7951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8000..8121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8011..8030)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8065..8084
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8295..9089)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8406..8425)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9016..9035
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9156..9232,1..253))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9170..9232,1..73)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(join(9224..9232,1..11))
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
       61 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
      121 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
      181 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
      241 ctaactgaca cacattccac agaattaatt cgcgttaaat ttttgttaaa tcagctcatt
      301 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
      361 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
      421 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
      481 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
      541 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
      601 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
      661 acccgccgcg cttaatgcgc cgctacaggg cgcgtgggga taccccctag agccccagct
      721 ggttctttcc gcctcagaag ccatagagcc caccgcatcc ccagcatgcc tgctattgtc
      781 ttcccaatcc tcccccttgc tgtcctgccc caccccaccc cccagaatag aatgacacct
      841 actcagacaa tgcgatgcaa tttcctcatt ttattaggaa aggacagtgg gagtggcacc
      901 ttccagggtc aaggaaggca cgggggaggg gcaaacaaca gatggctggc aactagaagg
      961 cacagtcgag gctgatcagc gagctctagc atttaggtga cactatagaa tagggccctc
     1021 tagatgcatg ctcgagcggc cgcactagtg attggtcctc acagcttggc agacccccat
     1081 gttgtaccct tgtccctgaa ggtggttgtg gcttggtggc aggtctgcag tggcttcctc
     1141 tcccctctcc cctcctactt gcaaagtagt gctgtgcctg ggatgctcca tcaggccagg
     1201 gaatagatgg cattgactgg agatgtggcc tctgagctct cattgccctc ggtctcctcc
     1261 ttgtcctttc tgacagtgta ctggccaata aaggagccat cttcgttgaa ctggccctcg
     1321 ccaccctcgc cgtagtccac taggctgtcg tcactctcct gctgcttgat ggtgccgtcc
     1381 agagatgtct ggctgccctg caggggcttg ttgtcctcgt cactgtagtc aaatgaacca
     1441 tcttcttctt tggggtcttc agggcccaag gggacatcct tcttttctcg cactgggtac
     1501 ttgccgccac gactcctctt gatgaagcag acgataagca ggatcagcac cagaagggca
     1561 atggcacaca tgagcccgat gaaccagccc tgggtggcga tgtcagtctg gttgttggtg
     1621 taagctgcag ttggagtagc ttcatttgga ggtgttgggg actcctccgt ggctgcttct
     1681 ccagagccca cctgcgtcct ggcactgagg gagaagcggt aacgggacac ggggtctgct
     1741 ctctgcaccg agaacttggt ctgattggga gagaagtttt ccaccatctg ctttcccagt
     1801 ttggttccat taaagggcac gtatctgagc gtgtatccaa tcaggatccc gttggggtgc
     1861 tctgggtgat cccattccag gttgatggtc tccaggttgg gctgtcggac tctgaaacgc
     1921 ctgggggcac tgggtactcc ttccggggtg gtgaattcct tggtttcact tcgaggccca
     1981 tcacctctcc cattgaccac aaccatctcc agcttgtagt tactgtaggg gaacaggcga
     2041 cccaccacgc cccgggggcg gtcgccaggg aagctggcct gctgtctctt ctgagacacc
     2101 cacaggttct tcagcaaact gctttccctc cagtagtaag ctcgatactc tctgagctgg
     2161 ccctggaccg tgtcagagta gacgcggttc cactgaaggc tgatggctgt gctgttcagg
     2221 actcggattt taacttcagt gggtgcagcc ctgggataat cttcccccga gtacccaatg
     2281 acggtttcag gctccgggcc tttcccaaag tcattttcag cctggactcg gatctcatag
     2341 ggtacgtaga caggggtctg ccccaccaca tagcgagagc cccacacggt gacattgttc
     2401 caagtctctc gggtttctct ccgtcgccac ttgacaatgt agcgcaggtt ggggccaaag
     2461 gcggaggtag cattcatagg cgtccatgtg atctccatat tgttttttcg tgtcccttcg
     2521 cccttcacat cactggggtt ggattcaggg ggtgccccgc ttgttcggta ccgctcggat
     2581 ggaaggctgg ggtggctgct cccaacctcg ttgacagcga tgactcggaa ctgatagttg
     2641 acatacgggg acagatggag gacggctgag ttgacactgc ctgggaactt ggagtggtca
     2701 tgccagactc ctggctggaa ctggtcctct tcaaactgga caacgtagtc tgtgatggga
     2761 ctgttgttat catcccccgg gatccaggtc agcctcacac tcctttccgc caggtcagtg
     2821 agctccaggt ccctgggtcg gtctggccgc cctagaacag tgaggtaggc ctttgccagg
     2881 tcctggtcca gctcggtgct ggccatgcac gtgtaactgc cttggtcccg ctctgccact
     2941 ccgaagatgg tcagggagtc atcttccttt ttcatcctgt ttccaatgta gagtggctcg
     3001 tcatccttca gccaggagac tgtgagtttc aaggaggggt catgcttcac acggcactcc
     3061 agctgcactg tggtgcccct cttggccacc tggtcttcag gcatcctgta gatcctggtg
     3121 gggtctttga cctccaggcg gacttgattt tcagcttttc ccaggatgtt ggtggccaca
     3181 caggtgtaga tgccttggtc ctctttgcga atcatcttga tttccaggct gccattttcg
     3241 tagacgtggt agttaccacc atccaggttg cttccttgcc cattcttaaa ccatcggagt
     3301 gttggaatgg gagacccaaa gaacggacag tccagtcgcg tccggttgta aaggatcacc
     3361 ctgatgagct ggttccgggg agacagcatc cgagggggta catctaatac gctgacgaag
     3421 gcattggcca gcaggtagcc atgttcgttg gatgtgttac actggtacac tgccctgctg
     3481 ctgatctgag tgtcccggaa gatgatagtg tctccggcca cctcacggtt ggggttgggt
     3541 ggtgccgatt gcaaagggtc tccattcacc agccactgga cggtcggctt cgggttccca
     3601 ttggctcgac acaccagcct cccatcttca ccaggagcca ggatcaagtt cttgggctca
     3661 tccagccagt atggggcagc ctttactctc accgagatcg tgtgccggat gctgcccatc
     3721 ttgttggagg ccaggcagaa atactcccca gagtcctctt cagagacatt ggtgatgcgc
     3781 agagccttgt taaaattctc gaacttggcc ttgtcagatg ggaggtcccc acctttcttg
     3841 taccatgcaa tatctggtgt tgggacccca gaggcaatgc actccagcag caggtccatg
     3901 ccgcgcagta ccatctgact gcttgacgtg ccctggggat acatgaagct aggtgttctt
     3961 tccgcaactc ctcgggcact atatatgtca gggtggtttc ttaaggacga gtcattatag
     4021 gggttgttgg tgaggacttt gagggtgaag ggattcttct gctgaatggt gtgggtgaag
     4081 tggaagcgtg cattgcagct gtagtcggtc tgcatgtcct gcagcatgac gttggagaag
     4141 tacaggtccc cgttgtgacc ctgggagaca cgcttgtcct gggtgatggg ctccatggag
     4201 ctgctcatcc agaagatgac gggggatggg aggccaggtg gggggttgca ctgcagggtt
     4261 aagggggcac cctcttgaac cacgacgggg tctaggtttt ccttgggcca caggggagat
     4321 ttggacacct gcaggcggat gcggttgcta agagctgtgc cgaatttgtt ccgggcaaag
     4381 cactggtact ccccttcgta ctcctcaggc cgcccaccac tgcggaagtc gatcaccaag
     4441 gtcccagacc tcctcctcat ggacacccgt gggtccttgg caatgttgaa gaacctgctg
     4501 ttgcgagtcc agtggaaact gggggcgggg ttccctttag cttcacattc aatcaggatg
     4561 ttatctcggg ggtccacgat gtggtccttc accgactgct tggtgatcgt tgggggttgg
     4621 gtcagatcca tcggaatctc aatggcccct ccgaggctga ggaggaagag gatgagggct
     4681 acgtggaccc agggtggcgc ctgctgcctg gccatcctca gcgccaggcc ctgctccgca
     4741 ctgcccagcc cacgggctct cgatgccttg cgccgttcct cgtgggcttt ccagacagtg
     4801 ctcagaatcc cgcggccatg gcggccggga gcatgcgacg tcgatctgca gaattccagc
     4861 acactggcgg ccgttactag tggatccgag ctcggtacca agcttgggtc tccctatagt
     4921 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     4981 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     5041 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     5101 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     5161 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     5221 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     5281 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     5341 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     5401 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     5461 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     5521 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     5581 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
     5641 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
     5701 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     5761 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6061 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6121 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6181 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6241 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6301 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6361 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6421 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6481 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6541 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6601 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6661 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6721 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6781 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6841 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7621 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7681 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7921 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     7981 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     8041 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     8101 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     8161 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     8221 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     8281 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     8341 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     8401 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     8461 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     8521 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     8581 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     8641 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     8701 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     8761 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     8821 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     8881 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     8941 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     9001 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     9061 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     9121 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     9181 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ct
//
