LOCUS       sequence-388185-       10638 bp    DNA     circular SYN 14-APR-2025
DEFINITION  study roles of human BET proteins in human pluripotency 
            reprogramming.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10638)
  AUTHORS   Hossain ME, Cevallos RR, Zhang R, Hu K
  TITLE     Attenuating iPSC reprogramming stress with dominant-negative BET 
            peptides.
  JOURNAL   iScience. 2022 Dec 28;26(1):105889. doi: 10.1016/j.isci.2022.105889.
            eCollection 2023 Jan 20.
  PUBMED    36691621
REFERENCE   2  (bases 1 to 10638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-12-28"; volume: "26(1):105889. doi"; pages: " 
            10.1016/j.isci.2022.105889. eCollection 2023 Jan 20"
COMMENT     Sequence Label: pLV-EF1a-AcGFP-P2A-hBRD2
FEATURES             Location/Qualifiers
     source          1..10638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2197..2408
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          2409..3347
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3304..3324
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3364..3373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3370..4086
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             4114..4170
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6606..7194
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6659..6679)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7077..7088)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7401..8034
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8163..8179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8163..8179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8176..8198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8187..8203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8211..8217,8218..8235,8236..8241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8256..8277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8394..8411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8565..9153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8645..8664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9324..10115,10116..10184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAVWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9947..9966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10185..10289)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10337..10471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10374..10393)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10428..10447
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgtgccc gtcagtgggc agagcgcaca tcgcccacag
     2221 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
     2281 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
     2341 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
     2401 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc
     2461 cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat cccgagcttc
     2521 gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct tcgcctcgtg
     2581 cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc
     2641 gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga tgacctgctg
     2701 cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg cacactggta
     2761 tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg
     2821 cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct caagctggcc
     2881 ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc
     2941 tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag
     3001 ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca cccacacaaa
     3061 ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag taccgggcgc
     3121 cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta ggttgggggg
     3181 aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa gttaggccag
     3241 cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga tcttggttca
     3301 ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg tcgtgacccg
     3361 gtcgccacca tggtgagcaa gggcgccgag ctgttcaccg gcatcgtgcc catcctgatc
     3421 gagctgaatg gcgatgtgaa tggccacaag ttcagcgtga gcggcgaggg cgagggcgat
     3481 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcctgtgccc
     3541 tggcccaccc tggtgaccac cctgagctac ggcgtgcagt gcttctcacg ctaccccgat
     3601 cacatgaagc agcacgactt cttcaagagc gccatgcctg agggctacat ccaggagcgc
     3661 accatcttct tcgaggatga cggcaactac aagtcgcgcg ccgaggtgaa gttcgagggc
     3721 gataccctgg tgaatcgcat cgagctgacc ggcaccgatt tcaaggagga tggcaacatc
     3781 ctgggcaata agatggagta caactacaac gcccacaatg tgtacatcat gaccgacaag
     3841 gccaagaatg gcatcaaggt gaacttcaag atccgccaca acatcgagga tggcagcgtg
     3901 cagctggccg accactacca gcagaatacc cccatcggcg atggccctgt gctgctgccc
     3961 gataaccact acctgtccac ccagagcgcc ctgtccaagg accccaacga gaagcgcgat
     4021 cacatgatct acttcggctt cgtgaccgcc gccgccatca cccacggcat ggatgagctg
     4081 tacaagtccg gactcagatc tcgaggatct ggagcaacaa acttctcact actcaaacaa
     4141 gcaggtgacg tggaggagaa tcccggcccc atgctgcaaa acgtgactcc ccacaataag
     4201 ctccctgggg aagggaatgc agggttgctg gggctgggcc cagaagcagc agcaccaggg
     4261 aagaggattc gaaaaccctc tctcttgtat gagggctttg agagccccac aatggcttcg
     4321 gtgcctgctt tgcaacttac ccctgccaac ccaccacccc cggaggtgtc caatcccaaa
     4381 aagccaggac gagttaccaa ccagctgcaa tacctacaca aggtagtgat gaaggctctg
     4441 tggaaacatc agttcgcatg gccattccgg cagcctgtgg atgctgtcaa actgggtcta
     4501 ccggattatc acaaaattat aaaacagcct atggacatgg gtactattaa gaggagactt
     4561 gaaaacaatt attattgggc tgcttcagag tgtatgcaag attttaatac catgttcacc
     4621 aactgttaca tttacaacaa gcccactgat gatattgtcc taatggcaca aacgctggaa
     4681 aagatattcc tacagaaggt tgcatcaatg ccacaagaag aacaagagct ggtagtgacc
     4741 atccctaaga acagccacaa gaagggggcc aagttggcag cgctccaggg cagtgttacc
     4801 agtgcccatc aggtgcctgc cgtctcttct gtgtcacaca cagccctgta tactcctcca
     4861 cctgagatac ctaccactgt cctcaacatt ccccacccat cagtcatttc ctctccactt
     4921 ctcaagtcct tgcactctgc tggacccccg ctccttgctg ttactgcagc tcctccagcc
     4981 cagccccttg ccaagaaaaa aggcgtaaag cggaaagcag atactaccac ccctacacct
     5041 acagccatct tggctcctgg ttctccagct agccctcctg ggagtcttga gcctaaggca
     5101 gcacggcttc cccctatgcg tagagagagt ggtcgcccca tcaagccccc acgcaaagac
     5161 ttgcctgact ctcagcaaca acaccagagc tctaagaaag gaaagctttc agaacagtta
     5221 aaacattgca atggcatttt gaaggagtta ctctctaaga agcatgctgc ctatgcttgg
     5281 cctttctata aaccagtgga tgcttctgca cttggcctgc atgactacca tgacatcatt
     5341 aagcacccca tggacctcag cactgtcaag cggaagatgg agaaccgtga ttaccgggat
     5401 gcacaggagt ttgctgctga tgtacggctt atgttctcca actgctataa gtacaatccc
     5461 ccagatcacg atgttgtggc aatggcacga aagctacagg atgtatttga gttccgttat
     5521 gccaagatgc cagatgaacc actagaacca gggcctttac cagtctctac tgccatgccc
     5581 cctggcttgg ccaaatcgtc ttcagagtcc tccagtgagg aaagtagcag tgagagctcc
     5641 tctgaggaag aggaggagga agatgaggag gacgaggagg aagaagagag tgaaagctca
     5701 gactcagagg aagaaagggc tcatcgctta gcagaactac aggaacagct tcgggcagta
     5761 catgaacaac tggctgctct gtcccagggt ccaatatcca agcccaagag gaaaagagag
     5821 aaaaaagaga aaaagaagaa acggaaggca gagaagcatc gaggccgagc tggggccgat
     5881 gaagatgaca aggggcctag ggcaccccgc ccacctcaac ctaagaagtc caagaaagca
     5941 agtggcagtg ggggtggcag tgctgcttta ggcccttctg gctttggacc ttctggagga
     6001 agtggcacca agctccccaa aaaggccaca aagacagccc cacctgccct gcctacaggt
     6061 tatgattcag aggaggagga agagagcagg cccatgagtt acgatgagaa gcggcagctg
     6121 agcctggaca tcaacaaatt acctggggag aagctgggcc gagttgtgca tataatccaa
     6181 gccagggagc cctctttacg tgattcaaac ccagaagaga ttgagattga ttttgaaaca
     6241 ctcaagccat ccacacttag agagcttgag cgctatgtcc tttcctgcct acgtaagaaa
     6301 ccccggaagc cctacaccat taagaagcct gtgggaaaga caaaggagga actggctttg
     6361 gagaaaaagc gggaattaga aaagcggtta caagatgtca gcggacagct caattctact
     6421 aaaaagcccc ccaagaaagc gaatgagaaa acagagtcat cctctgcaca gcaagtagca
     6481 gtgtcacgcc ttagcgcttc cagctccagc tcagattcca gctcctcctc ttcctcgtcg
     6541 tcgtcttcag acaccagtga ttcagactca ggctaaggga tccaccggat ctaggcgtct
     6601 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6661 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     6721 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     6781 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     6841 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     6901 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     6961 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7021 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7081 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7141 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7201 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7261 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7321 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7381 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7441 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     7501 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     7561 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     7621 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     7681 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     7741 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     7801 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     7861 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7921 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7981 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8041 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8101 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
     8161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     8281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     8341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     8401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     8461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     8521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     8581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     8641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     8701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     8761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     8821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     8881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     8941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     9301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     9361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     9421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     9481 gctccagatt tatcagcaat aaaccagaca gccggaaggg ccgagcgcag aagtggtcct
     9541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     9601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     9661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     9721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     9781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     9841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     9901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    10321 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
    10381 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
    10441 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
    10501 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
    10561 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
    10621 tactacaaac ttagtagt
//
