LOCUS       sequence-388194-        3186 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Cloning of promoter of interest in front of mkate2 reporter gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Bernauw AJ, Crabbe V, Ryssegem F, Willaert R, Bervoets I, Peeters E
  TITLE     Molecular mechanisms of regulation by a beta-alanine-responsive 
            Lrp-type transcription factor from Acidianus hospitalis.
  JOURNAL   Microbiologyopen. 2023 Jun;12(3):e1356. doi: 10.1002/mbo3.1356.
  PUBMED    37379425
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiologyopen."; date: "2023-06"; volume: "12(3):e1356. doi"; 
            pages: " 10.1002/mbo3.1356"
COMMENT     Sequence Label: pPRC6
FEATURES             Location/Qualifiers
     source          1..3186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(153..947)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(264..283)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     874..893
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     RBS             1407..1415
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     rep_origin      2442..3030
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2931..2950
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgagatcc ccgcgctgga ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc
       61 caacctttca tagaaggcgg cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc
      121 ttggtcggtc atttcgaacc ccagagtccc gctcagaaga actcgtcaag aaggcgatag
      181 aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc
      241 cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc ctgatagcgg
      301 tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt ttccaccatg
      361 atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc gggcatgcgc
      421 gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc gtccagatca
      481 tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg atgtttcgct
      541 tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat tgcatcagcc
      601 atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg ccccggcact
      661 tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa
      721 ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg
      781 gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga cagccggaac
      841 acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa tagcctctcc
      901 acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcct
      961 catcctgtct cttgatcaga tcttgatccc ctgcgccatc agatccttgg cggcaagaaa
     1021 gccatccagt ttactttgca gggcttccca accttaccag agggcgcccc agctggcaat
     1081 tccggttcgc ttgctgtcca taaaaccgcc cagtctagct atcgccatgt aagcccactg
     1141 caagctacct gctttctctt tgcgcttgcg ttttcccttg tccagatagc ccagtagctg
     1201 acattcatcc ggggtcagca ccgtttctgc ggactggctt tctacgtgtt ccgcttcctt
     1261 tagcagccct tgcgccctga gtgcttgcgg cagcgtgggg gatcttgaag ggatccaaaa
     1321 aaaaaccccg ccgaagcggg gttttttttt gacgatggta gtcagctgcc tactaagctg
     1381 tctagataca atgcgatatc atcgcctaag gaggttaagc atatggttag cgagctgatc
     1441 aaagaaaaca tgcacatgaa actgtatatg gaaggcaccg tgaataacca ccactttaaa
     1501 tgtaccagcg aaggtgaagg taaaccgtat gaaggcaccc agaccatgcg tattaaagca
     1561 gttgaaggtg gtccgctgcc gtttgcattt gatattctgg caaccagctt tatgtatggc
     1621 agcaaaacct ttattaacca tacccagggt atcccggatt ttttcaaaca gagctttccg
     1681 gaaggtttta cctgggaacg tgttaccacc tatgaagatg gtggtgttct gaccgcaacc
     1741 caggatacca gtctgcagga tggttgtctg atttataatg tgaaaattcg cggtgtgaac
     1801 tttccgagca atggtccggt tatgcagaaa aaaaccctgg gttgggaagc aagcaccgaa
     1861 accctgtatc cggcagatgg tggtctggaa ggtcgtgcag atatggcact gaaactggtt
     1921 ggtggtggtc atctgatttg caatctgaaa accacctatc gtagcaaaaa accggcaaaa
     1981 aatctgaaaa tgcctggcgt gtattatgtt gatcgtcgtc tggaacgtat taaagaggca
     2041 gataaagaaa cctatgtgga acagcatgaa gttgcagttg cacgttattg tgatctgccg
     2101 agcaaactgg gtcaccgctg ataaccatgg gctagcggtt tgaagggtat tggtcggtca
     2161 gtttcacctg atttacgtaa aaacccgctt cggcgggttt ttgcttttgg aggggcagaa
     2221 agatgaatga ctgtcctttt attggagagg tggacaagtg gcatcagagt tcactcttaa
     2281 ttctgaacat acccgtcttt ttcgcctctt ttacgtgatt aactccagcg ctgggcggcc
     2341 gcttactaga acggtaccga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2401 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2461 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2521 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2581 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2641 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2701 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2761 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2821 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2881 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2941 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     3001 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     3061 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     3121 gataaccgta ttaccgcctt tgagtgagag agcgcttttg aagctggggt gggcgaagaa
     3181 ctccag
//
