LOCUS       sequence-388237-        9960 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9960)
  AUTHORS   Garg R, Koo CY, Infante E, Giacomini C, Ridley AJ, Morris JDH
  TITLE     Rnd3 interacts with TAO kinases and contributes to mitotic cell 
            rounding and spindle positioning.
  JOURNAL   J Cell Sci. 2020 Mar 16;133(6):jcs235895. doi: 10.1242/jcs.235895.
  PUBMED    32041905
REFERENCE   2  (bases 1 to 9960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2020-03-16"; volume: "133(6):jcs235895. doi"; pages: "
            10.1242/jcs.235895"
COMMENT     Sequence Label: CB6-GFP-TAOK1
FEATURES             Location/Qualifiers
     source          1..9960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(944..946,947..1657)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKGDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(986..1007)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     1594..1615
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1668..1684
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1669..1685
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4719..5341
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4918..4937)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5365..5385)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5383..5680
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5734..6528
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5788..5807)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6398..6417
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7180..7314
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7217..7236)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7271..7290
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7319..7338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7320..7338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7352..7368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7352..7368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7365..7387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7376..7392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7400..7406,7407..7424,7425..7430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7445..7466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7583..7600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7754..8342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7834..7853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8513..9304,9305..9373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9136..9155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9374..9478)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9505..9933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9592..9611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9802..9823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attaattcag atctggtacc accatgagca agggcgagga
      961 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     1021 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1081 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     1141 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     1201 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaagggcg acggcaacta
     1261 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     1321 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     1381 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     1441 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     1501 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     1561 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1621 cgccgggatc actctcggca tggacgagct gtacaagggc ggccgcctcg aggtcgacgg
     1681 tatcgataag cttgatatcg aattcatgcc atcaactaac agagcaggca gcctgaagga
     1741 ccctgaaatt gcagagctct tcttcaaaga agatccagag aagctcttca cagatctcag
     1801 agaaattggc catggaagct ttggagcagt gtattttgca cgagatgtgc gtaccaatga
     1861 agtggtggcc atcaagaaaa tgtcttatag tggaaagcag tctactgaga aatggcagga
     1921 tattattaag gaagtcaagt ttctacaaag aataaaacat cccaacagta tagaatacaa
     1981 aggctgttat ttacgtgaac acacagcatg gcttgtaatg gaatattgtt taggatctgc
     2041 ttcggattta ctagaagttc acaaaaagcc attacaagaa gtggaaatag cagcaattac
     2101 acatggtgct cttcagggat tagcctactt acattctcat actatgattc atagagatat
     2161 caaagcagga aatatccttc tgacagaacc aggccaggtg aaacttgctg actttggctc
     2221 tgcttccatg gcatcacctg ccaattcctt tgtgggaacg ccgtattgga tggccccaga
     2281 agtaatttta gccatggatg aaggacaata tgatggcaaa gtagatgtgt ggtctcttgg
     2341 aataacatgt attgaactag cggaaaggaa gcctccttta tttaatatga atgcaatgag
     2401 tgccttatat cacatagccc aaaatgaatc ccctacacta cagtctaatg aatggtctga
     2461 ttattttcgc aactttgtag attcttgcct ccagaaaatc cctcaagatc gacctacatc
     2521 agaggaactt ttaaagcaca tatttgttct tcgggagcgc cctgaaaccg tgttaataga
     2581 tctcattcag aggacaaagg atgcagtaag agagctggac aatctgcagt atcgaaagat
     2641 gaagaaactc cttttccagg aggcacataa tggaccagca gtagaagcac aggaagaaga
     2701 agaggaacaa gatcatggtg ttggccggac aggaacagtt aatagtgttg gaagtaatca
     2761 atccattccc agcatgtcca tcagtgccag cagccaaagc agtagtgtta acagtcttcc
     2821 agatgtctca gatgacaaga gtgagctaga catgatggag ggagaccaca cagtgatgtc
     2881 taacagttct gttatccatt taaaaccaga ggaagaaaat tacagagaag agggagatcc
     2941 tagaacaaga gcatcagatc cacaatctcc accccaagta tctcgtcaca aatcacacta
     3001 tcgtaatcga gaacactttg ctactatacg gacagcatca ctggttacga ggcaaatgca
     3061 agaacatgag caggactctg agcttagaga acaaatgtct ggctataagc gaatgaggcg
     3121 acaacatcaa aagcaactga tgactctgga aaacaagcta aaggctgaga tggatgaaca
     3181 tcgcctcaga ttagacaaag atcttgaaac tcagcgtaac aattttgctg cagaaatgga
     3241 gaaacttatc aagaaacacc aggctgctat ggagaaagag gctaaagtga tgtccaatga
     3301 agagaaaaaa tttcagcaac atattcaggc ccaacagaag aaagaactga atagttttct
     3361 cgagtcccag aaaagagagt ataaacttcg aaaagagcag cttaaagagg agctaaatga
     3421 aaaccagagt acccccaaaa aagaaaaaca ggagtggctt tcaaagcaga aggagaatat
     3481 acagcatttc caagcagaag aagaagctaa ccttcttcga cgtcaaagac aatacctaga
     3541 gctggaatgc cgtcgcttca agagaagaat gttacttggg cgtcataact tagagcagga
     3601 ccttgtcagg gaggagttaa acaaaagaca gactcagaag gacttagagc atgccatgct
     3661 actccgacag catgaatcta tgcaagaact ggagttccgc cacctcaaca caattcagaa
     3721 gatgcgctgt gagttgatca gattacagca tcaaactgag ctcactaacc agctggaata
     3781 taataagcga agagaacgag aactaagacg aaagcatgtc atggaagttc gacaacagcc
     3841 taagagtttg aagtctaaag aactccaaat aaaaaagcag tttcaggata cctgcaaaat
     3901 ccaaaccaga cagtacaaag cattaagaaa tcacctgctg gagactacac caaagagtga
     3961 gcacaaagct gttctgaaac ggctcaagga ggaacagacc cggaaattag ctatcttggc
     4021 tgagcagtat gatcacagca ttaatgaaat gctctccaca caagccctgc gtttggatga
     4081 agcacaggaa gcagagtgcc aggttttgaa gatgcagctg cagcaggaac tggagctgtt
     4141 gaatgcgtat cagagcaaaa tcaagatgca agctgaggca caacatgatc gagagcttcg
     4201 cgagcttgaa cagagggtct ccctccggag ggcactctta gaacaaaaga ttgaagaaga
     4261 gatgttggct ttgcagaatg agcgcacaga acgaatacga agcctgttgg aacgtcaagc
     4321 cagagagatt gaagcttttg actctgaaag catgagacta ggttttagta atatggtcct
     4381 ttctaatctc tcccctgagg cattcagcca cagctacccg ggagcttctg gttggtcaca
     4441 caaccctact gggggtccag gacctcactg gggtcatccc atgggtggcc caccacaagc
     4501 ttggggccat ccaatgcaag gtggacccca gccatggggt cacccttcag ggccaatgca
     4561 aggggtacct cgaggtagca gtatgggagt ccgcaatagc ccccaggctc tgaggcggac
     4621 agcttctggg ggacggacag agcagggcat gagcagaagc acgagtgtca cttcacaaat
     4681 atccaatggg tcacacatgt cttatacata aggatcccgg gtggcatccc tgtgacccct
     4741 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     4801 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     4861 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     4921 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     4981 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     5041 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     5101 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     5161 tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa ctataccagc
     5221 aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg ggacatttga
     5281 gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg cctgttgaat
     5341 tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     5401 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     5461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     5521 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     5581 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     5641 cctctgagct attccagaag tagtgaggag gcttttttgg aggaattgat cagcttggga
     5701 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5761 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5821 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5881 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5941 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     6001 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     6061 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     6121 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     6181 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     6241 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     6301 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     6361 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     6421 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     6481 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     6541 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     6601 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     6661 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc cgggctcgat cccctcgcga
     6721 gttggttcag ctgctgcctg aggctggacg acctcgcgga gttctaccgg cagtgcaaat
     6781 ccgtcggcat ccaggaaacc agcagcggct atccgcgcat ccatgccccc gaactgcagg
     6841 agtggggagg cacgatggcc gctttggtcg acccggacgg gacgctcctg cgcctgatac
     6901 agaacgaatt gcttgcaggc atctcatgag tgtgtcttcc cgttttccgc ctgaggtcac
     6961 tgcgtggatg gagcgctggc gcctgctgcg cgacggcgag ctgctcacca cccactcgcc
     7021 aagctggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gccgatcata
     7081 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     7141 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     7201 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     7261 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaattcc
     7321 ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     7381 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7441 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7501 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7561 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7621 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7681 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7921 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8161 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8221 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8281 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8341 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8401 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8461 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8521 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8581 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8641 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8701 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8761 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8821 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8881 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8941 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9001 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9061 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9481 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9541 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9601 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9661 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9721 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     9781 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     9841 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     9901 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
//
