LOCUS       sequence-389043-        6089 bp    DNA     circular SYN 14-APR-2025
DEFINITION  bacteria reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Schulte M, Olschewski K, Hensel M
  TITLE     The protected physiological state of intracellular Salmonella 
            enterica persisters reduces host cell-imposed stress.
  JOURNAL   Commun Biol. 2021 May 4;4(1):520. doi: 10.1038/s42003-021-02049-6.
  PUBMED    33947954
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-05-4"; volume: "4(1):520. doi"; pages: " 
            10.1038/s42003-021-02049-6"
COMMENT     Sequence Label: p5084
FEATURES             Location/Qualifiers
     source          1..6089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..48
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             89..766
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASTEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(219..237)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(269..289)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     487..506
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     681..704
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             2138..2329
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(2323..2345)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    2431..2571
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     2486..2505
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2586..2603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2757..3345)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2837..2856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3516..4307,4308..4376))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4139..4158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4377..4468)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(4487..4514)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4606..4692)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4845..4862
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(4904..5617)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(5956..5975)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgactc agggcccact
       61 agtggtaccc gggaaagagg agaaaagtat ggcatccacc gaggacgtca tcaaggagtt
      121 catgcgcttc aaagtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      181 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      241 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
      301 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
      361 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      421 ctccctgcag gacggctgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
      481 cgacggcccc gtaatgcaga agaagactat gggctgggag ccctccaccg agcgcctgta
      541 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
      601 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc aactgcccgg
      661 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
      721 ggagcagtac gaacgcaccg agggccgcca ccacctgttc ctgtagccat ggatcgatag
      781 ctggtcgaca accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
      841 gcttcctaat gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca
      901 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
      961 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     1021 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     1081 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     1141 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     1201 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     1261 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     1321 gatcgctcgc ggctcttacc agcctaactt cgatcattgg accgctgatc gtcacggcga
     1381 tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat
     1441 accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa
     1501 tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt
     1561 cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc
     1621 catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg
     1681 catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta
     1741 gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc
     1801 gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg
     1861 gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc
     1921 ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct
     1981 ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc
     2041 atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac
     2101 ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc
     2161 ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga
     2221 gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct
     2281 ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2341 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2401 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
     2461 cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2521 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     2581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     2881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     2941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3181 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     3901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     3961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     4141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4441 tttgaatgta tttagaaaaa taaacaaaag agtttgtaga aacgcaaaaa ggccatccgt
     4501 caggatggcc ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4561 ccctccgggc cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag
     4621 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt
     4681 tcgttttatt tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat
     4741 cggcgctacg gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac
     4801 tgccgccagg caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca
     4861 ggctgaaaat cttctctcat ccgccaaaac agaagtttta ttatttatac agttcatcca
     4921 tgccatgcgt aatacccgcc gcggtcacaa attccagcag caccatatgg tcgcgttttt
     4981 cgttcggatc tttgctcagg acgctctggg tgctcagata atggttatcc ggcagcagca
     5041 ccgggccatc gccaatcggg gtgttctgct gataatgatc cgccagctgc acgctgccat
     5101 cttccacgtt gtggcggatt ttaaagttcg ctttgatgcc gtttttctgt ttatccgcgg
     5161 taatatacac gttatggctg ttaaagttgt attccagttt atggcccagg atgttgccat
     5221 cttctttaaa atcgatgcct ttcagttcaa tgcggttcac cagggtatcg ccttcaaatt
     5281 tcacttccgc acgggtttta taggtgccat catctttaaa gctaatggtg cgttcctgca
     5341 catagccttc cggcatcgcg cttttaaaaa aatcatgctg tttcatatga tccggataac
     5401 gcgcaaagca ctgcacgcca taggtcaggg tggtcaccag ggtcggccac ggcaccggca
     5461 gtttgcccgt ggtgcaaatg aatttcaggg tcagtttgcc gttggtcgca tcgccttcgc
     5521 cttcgccacg cacgctaaat ttatggccgt tcacatcgcc atccagttcc accagaatcg
     5581 gcaccacacc ggtaaacagt tcttcgcctt tgcgcatgag ctattctccc gaaagcgtta
     5641 attggcgtta aggttgtaac gagacgcatc tttgcacaca ataacaacat taatgtatct
     5701 ggatttaacc ataagaaata tttgggcagt cgtctgcttt tcaatcgaaa ttgttgattt
     5761 tatgttaagc cgcggagcgg tagtgtgatt ttttccaggg gtgggaatag gggatattca
     5821 ggagaaaatg tgccacatat ccgtcagtta tgttgggtta gcttactgtg cctgagcagt
     5881 tctgcggtag ccgcaaatgt tcgtctgaaa gtcgaagggc cggcatcacc ggcgccacag
     5941 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     6001 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     6061 tgttgggcgc catctccttc catggacgt
//
