LOCUS       sequence-389096-       10895 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in 
            the RUSH system; visualized with HaloTag. Reporter only (no hook). 
            SYT1 is mutated to disrupt palmitoylation and glycosylation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10895)
  AUTHORS   Watson ET, Pauers MM, Seibert MJ, Vevea JD, Chapman ER
  TITLE     Synaptic vesicle proteins are selectively delivered to axons in 
            mammalian neurons.
  JOURNAL   Elife. 2023 Feb 2;12:e82568. doi: 10.7554/eLife.82568.
  PUBMED    36729040
REFERENCE   2  (bases 1 to 10895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2023-02-2"; volume: "12:e82568. doi"; pages: " 
            10.7554/eLife.82568"
COMMENT     Sequence Label: pFsynW SYT1-PGM reporter
FEATURES             Location/Qualifiers
     source          1..10895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(119..910,911..979))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     742..761
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(980..1084)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1159..1178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1350..1729
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1731..1929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1884..1904
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1947..2127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2174..2299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2792..3025
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3210..3254
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3552..3669
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3714..4160
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      4177..4186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4297..4410
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             5758..6642
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             6649..6672
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    6699..7287
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6752..6772)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7170..7181)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7290..7306)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7291..7307)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7812..7992
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8018..8035)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8024..8248
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8294..8722
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8381..8400)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8591..8612
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8731..8751)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8736..9065
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8916..9051
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8978..8997
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9113..9160
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9179..9553
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9683..9804
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9720..9739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9774..9793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9853..9869)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9853..9869)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9866..9888)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9877..9893
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9901..9907,9908..9925,9926..9931))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9946..9967
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10084..10101)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10255..10843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10335..10354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
       61 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
      121 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
      181 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
      241 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
      301 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
      361 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
      421 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
      481 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
      541 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
      601 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
      661 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
      721 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
      781 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      841 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      901 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      961 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     1021 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     1081 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
     1141 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
     1201 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
     1261 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
     1321 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
     1381 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     1441 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     1501 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     1561 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     1621 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     1681 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     1741 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     1801 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     1861 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     1921 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2101 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     2161 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     2221 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     2281 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     2341 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     2401 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     2461 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     2521 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     2581 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     2641 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     2701 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     2761 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     2821 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     2881 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     2941 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     3001 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     3061 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     3121 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     3181 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     3241 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3301 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     3361 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     3421 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     3481 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     3541 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3601 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3661 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taaagtgcaa
     3721 gtggttttag gaccaggatg aggcggggtg ggggtgccta cctgacgacc gaccccgacc
     3781 cactggacaa gcacccaacc cccattcccc aaattgcgca tcccctatca gagaggggga
     3841 ggggaaacag gatgcggcga ggcgcgtgcg cactgccagc ttcagcaccg cggacagtgc
     3901 cttcgccccc gcctggcggc gcgcgccacc gccgcctcag cactgaaggc gcgctgacgt
     3961 cactcgccgg tcccccgtaa actccccttc ccggccacct tggtcgcgtc cgcgccgccg
     4021 ccggcccagc cggaccgcac cacgcgaggc gcgagatagg ggggcacggg cgcgaccatc
     4081 tgcgctgcgg cgccggcgac tcagcgctgc ctcagtctgc ggtgggcagc ggaggagtcg
     4141 tgtcgtgcct gagagcgcag gtacggctag gccactgcca ccatggacag taagggtagc
     4201 tcccagaaag gcagcaggct gcttttgctt ctcgttgtat ctaatctttt gctctgccag
     4261 ggcgtagttt ccgacattgg aaccacgagg ggttccatgg atgagaagac gacgggttgg
     4321 cggggtggcc atgtggtaga gggcttggca ggtgagcttg aacagctcag agctaggttg
     4381 gaacaccatc cacaaggtca acgagagccg ggcagcggct caagcgggtc ctccggaagt
     4441 tctggttcat ctatggtgag tgccagtcat cctgaggccc tggccgcccc tgtcgccgct
     4501 gttgcgaccc ttgtcccaca ccaggccact gagccagcca gtcctgggga agggaaggaa
     4561 gatgcctttt ccaagctgaa gcagaagttt atgaatgagc tgcataaaat tccattgcca
     4621 ccgtgggcct taatagccat agccatagtt gcggtccttc tagtcgtgac cgcagccttc
     4681 gctgtcgcta agaaagcttt gttcaaaaag aaaaacaaga agaaggggaa ggaaaaggga
     4741 ggaaagaacg ccattaacat gaaagacgtg aaagacttag ggaagaccat gaaggatcag
     4801 gcccttaagg atgacgatgc tgaaaccgga ctgactgatg gagaagaaaa ggaagagccc
     4861 aaggaagagg agaaactggg aaagctccaa tattcactgg actatgactt ccagaataac
     4921 cagctgttgg tgggaatcat ccaggctgct gaactgcccg ccctggacat ggggggtaca
     4981 tccgatccat acgtcaaagt cttcctgctg cctgacaaaa agaagaaatt tgagactaaa
     5041 gtccaccgga aaaccctcaa tccagtcttc aatgaacaat ttactttcaa ggtaccctac
     5101 tcggaattag gtggcaaaac cctggtgatg gctgtgtatg actttgatcg cttctccaag
     5161 cacgacatca tcggagagtt caaagttcct atgaacaccg tggattttgg ccatgtgacc
     5221 gaggagtggc gcgatctcca gagcgctgag aaagaagagc aagagaaact gggtgacatc
     5281 tgcttctccc tccgctacgt ccctactgcc ggcaaactga ctgttgtcat tctggaagcc
     5341 aagaacctga agaagatgga tgtgggtggc ttatctgatc cctacgtgaa gatccacctg
     5401 atgcagaacg gtaagaggct gaagaagaaa aagacgacga ttaagaagaa cacactcaac
     5461 ccctactaca acgagtcctt cagctttgaa gttccgttcg agcaaatcca gaaagtgcaa
     5521 gtggtggtaa ctgttttgga ctatgacaag attggcaaga acgacgccat cggaaaagtc
     5581 ttcgttggtt acaacagcac tggggcggag ctgcgacact ggtcagacat gctggccaac
     5641 ccccggcgac ccatcgcaca gtggcacact ctgcaggtag aggaggaggt tgatgccatg
     5701 ctggctgtca agaagggatc cggaagttcg gggtccagcg gttcttcagg cagttccgaa
     5761 atcggtactg gctttccatt cgacccccat tatgtggaag tcctgggcga gcgcatgcac
     5821 tacgtcgatg ttggtccgcg cgatggcacc cctgtgctgt tcctgcacgg taacccgacc
     5881 tcctcctacg tgtggcgcaa catcatcccg catgttgcac cgacccatcg ctgcattgct
     5941 ccagacctga tcggtatggg caaatccgac aaaccagacc tgggttattt cttcgacgac
     6001 cacgtccgct tcatggatgc cttcatcgaa gccctgggtc tggaagaggt cgtcctggtc
     6061 attcacgact ggggctccgc tctgggtttc cactgggcca agcgcaatcc agagcgcgtc
     6121 aaaggtattg catttatgga gttcatccgc cctatcccga cctgggacga atggccagaa
     6181 tttgcccgcg agaccttcca ggccttccgc accaccgacg tcggccgcaa gctgatcatc
     6241 gatcagaacg tttttatcga gggtacgctg ccgatgggtg tcgtccgccc gctgactgaa
     6301 gtcgagatgg accattaccg cgagccgttc ctgaatcctg ttgaccgcga gccactgtgg
     6361 cgcttcccaa acgagctgcc aatcgccggt gagccagcga acatcgtcgc gctggtcgaa
     6421 gaatacatgg actggctgca ccagtcccct gtcccgaagc tgctgttctg gggcacccca
     6481 ggcgttctga tcccaccggc cgaagccgct cgcctggcca aaagcctgcc taactgcaag
     6541 gctgtggaca tcggcccggg tctgaatctg ctgcaagaag acaacccgga cctgatcggc
     6601 agcgagatcg cgcgctggct gtctactctg gagatttccg gttcttcaga ctacaaagac
     6661 gatgacgaca agtaaaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     6721 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     6781 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     6841 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     6901 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     6961 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     7021 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     7081 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     7141 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     7201 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     7261 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
     7321 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7381 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7441 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7501 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7561 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7621 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7681 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7741 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7801 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7861 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7921 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7981 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     8041 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     8101 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     8161 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     8221 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
     8281 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8341 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8401 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8461 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     8521 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     8581 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     8641 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     8701 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8761 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8821 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8881 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8941 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9001 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9061 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
     9121 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
     9181 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
     9241 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
     9301 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
     9361 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
     9421 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
     9481 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
     9541 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
     9601 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     9661 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     9721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9781 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     9841 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     9901 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     9961 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    10021 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    10081 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    10141 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    10201 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    10261 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    10321 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    10381 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    10441 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    10501 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    10561 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    10621 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    10681 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    10741 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    10801 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    10861 ttctacgggg tctgacgctc agtggaacga aaact
//
