LOCUS       sequence-389121-       19325 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Mammalian cell expression of wt FLAG-BIRC6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19325)
  AUTHORS   Hunkeler M, Jin CY, Fischer ES
  TITLE     Structures of BIRC6-client complexes provide a mechanism of 
            SMAC-mediated release of caspases.
  JOURNAL   Science. 2023 Mar 17;379(6637):1105-1111. doi: 
            10.1126/science.ade5750. Epub 2023 Feb 9.
  PUBMED    36758104
REFERENCE   2  (bases 1 to 19325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.ade5750"; journalName: "Science"; date: 
            "2023-03-17- 17"; volume: "379"; issue: "6637"; pages: "1105-1111"
COMMENT     Sequence Label: pDEST_CMV_N_Flag_BIRC6
FEATURES             Location/Qualifiers
     source          1..19325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    995..1019
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(15608..15632)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    15666..16288
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(15865..15884)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(16312..16332)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        16317..16646
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      16497..16632
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(16684..16703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(16685..16703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(16717..16733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16717..16733)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(16730..16752)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    16741..16757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(16765..16771,16772..16789,16790..16795))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    16810..16831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(16948..16965)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17119..17707)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17199..17218)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(17878..18669,18670..18738))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     18501..18520
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(18739..18843)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      18870..19298
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(18957..18976)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     19167..19188
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      841 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
      961 tgcggccgcg aattcatcga tagatctgat atcaacaagt ttgtacaaaa aagcaggctt
     1021 aaccatggta acgggtgggg gtgcggcacc acccggcacg gtcactgagc cgctgccatc
     1081 cgtgatagtg ctttctgccg gtcgaaagat ggccgcagct gctgccgccg cctccggacc
     1141 tggttgttcc tctgccgcag gagctggggc tgcgggggtt agcgaatggc tggtactgcg
     1201 agatggctgc atgcattgcg atgcagacgg gctgcatagt ctctcctacc acccagcgct
     1261 taatgctatt ctggcggtta cctcccgcgg cacgatcaag gtcattgatg gaacaagtgg
     1321 ggcaacgctc caagcctccg cgctgtcagc caaaccaggc ggacaggtta aatgccagta
     1381 catttcagca gtagataagg ttattttcgt cgatgactat gccgtggggt gtcggaaaga
     1441 tttgaatggg atactcctgc tggatacggc gctccagacc ccggtcagta agcaggacga
     1501 cgttgtccag ctggaactcc ccgtgaccga agcacagcaa ttgctctctg cctgcctcga
     1561 gaaagtagat atcagcagca ccgaaggtta cgacctcttc attacgcagc ttaaggatgg
     1621 cctgaagaat accagccacg agacagcagc gaatcacaag gtagctaaat gggcgaccgt
     1681 aacattccac cttcctcacc acgtgcttaa atctatagct tctgctatag tcaacgagct
     1741 gaaaaaaata aatcagaacg tcgctgccct tccagtggct agttcagtta tggatcgctt
     1801 gagctacctc ttgcctagcg cgcgcccaga gttgggagtt ggccccgggc ggtccgtcga
     1861 tcgatcactt atgtactctg aagctaatcg gagagaaact tttacctcct ggccccatgt
     1921 cggctatcga tgggctcagc cagacccgat ggcccaggct ggattttacc atcaacctgc
     1981 gtcttcaggt gatgacagag cgatgtgctt tacctgctca gtatgcctgg tatgttggga
     2041 gccgaccgat gagccttgga gcgagcatga acggcattca ccgaattgcc cgtttgtcaa
     2101 gggagaacac acgcagaatg taccactctc tgttactctg gctacgtccc ccgcacaatt
     2161 tccgtgtacc gacggaacag atcgaatatc atgctttggg agcgggtcct gtccacactt
     2221 ccttgcagcg gctaccaaac gcggaaaaat atgcatctgg gatgtctcaa agcttatgaa
     2281 agtccatttg aaattcgaaa tcaatgcata tgaccctgcc atcgtacagc aacttatctt
     2341 gagtggcgat ccctcctccg gtgtagatag ccgaagacct acgcttgcgt ggctcgagga
     2401 ttcatcatct tgctctgaca tcccgaaact cgaaggagat agtgatgacc tgctcgagga
     2461 ttcagactcc gaagaacaca gcaggtccga tagcgttact gggcatacct cacaaaaaga
     2521 ggccatggaa gtctccctcg acattaccgc gctttctatc ctccaacagc ctgaaaaact
     2581 ccaatgggaa atagtggcca atgtgcttga agacactgta aaagatcttg aggaactggg
     2641 agcgaatccc tgcctgacta actccaaatc tgaaaaaacg aaggagaagc accaggagca
     2701 gcataacatt cctttcccct gtctgcttgc gggcggactt ctgacataca aatccccagc
     2761 tacttcacct attagttcaa attctcatag gtcactcgat ggcctttctc gaacgcaggg
     2821 tgaatccatt agtgagcagg gttcaacgga taatgaatcc tgtactaata gcgagctcaa
     2881 ttctcccttg gtcagacgca ctttgcctgt attgcttctg tattctatca aggagagcga
     2941 tgaaaaagca gggaaaatat ttagccaaat gaacaatatt atgtccaaaa gtcttcatga
     3001 tgatggcttc acggtcccgc aaattatcga gatggaactg gattcacaag aacaattgct
     3061 ccttcaagac ccacctgtta catatattca acaattcgca gacgccgcag caaatctcac
     3121 gagccctgat tccgagaaat ggaattcagt attccccaag cctggtacgc tggttcagtg
     3181 tttgcgactg cctaaattcg cggaagaaga gaatctttgc atagactcaa taacgccttg
     3241 tgcagatggt attcatttgc tggtgggact gcgcacgtgc ccagtcgaga gcctctcagc
     3301 aataaaccaa gttgaagctt tgaacaacct gaacaagctg aatagcgcac tgtgtaatag
     3361 acggaaaggc gaactcgaat ccaatttggc ggttgtcaac ggtgcaaata tctccgttat
     3421 ccaacatgaa tcccccgctg atgttcaaac tcccctcata attcagcctg aacaaaggaa
     3481 cgtaagtggg ggatatttgg tgctctacaa aatgaattac gctacacgca tagtaactct
     3541 tgaggaagag cccataaaga ttcaacatat caaagacccg caagacacga tcacatcact
     3601 cattctcctc ccccccgaca tcctcgacaa tcgggaagac gactgtgagg aaccaataga
     3661 ggacatgcaa cttacgtcca aaaacggctt cgaacgggag aagacttcag acatcagtac
     3721 actgggacac ctggtaatta cgactcaagg cggctacgtt aagatccttg atttgagcaa
     3781 tttcgaaatc ctggcgaagg ttgaaccgcc caaaaaagaa ggaacagaag agcaagacac
     3841 attcgtctca gtgatctact gtagtggcac tgaccgcctt tgcgcgtgca ccaagggcgg
     3901 ggaacttcat tttctgcaga taggaggcac atgtgacgac atagacgagg ccgacatatt
     3961 ggtcgatggg agccttagta aggggataga accgtcttct gagggctcca agccccttag
     4021 caacccttcc agtccgggta tctctggcgt tgacctgttg gtagatcaac cgttcacgct
     4081 cgaaatcttg acttcactgg tggagctgac tcgatttgag accctgactc caagattctc
     4141 cgcaactgtt cctccctgtt gggtagaagt ccagcaagag cagcagcagc gacggcaccc
     4201 acaacacctc caccaacaac atcacgggga tgccgcgcaa catacgagaa cgtggaaact
     4261 gcagaccgat tcaaacagtt gggacgaaca cgtattcgaa ttggtcctcc ctaaggcatg
     4321 catggttgga catgtcgact tcaaatttgt cctcaatagt aacattacta atattccgca
     4381 aattcaagtt actctgctga aaaacaaggc accggggttg ggaaaagtca atgcgttgaa
     4441 tatagaagtc gagcaaaacg gaaaaccctc attggttgac cttaatgaag agatgcaaca
     4501 catggatgtt gaggaaagtc agtgcctcag actttgtccc ttccttgaag accacaaaga
     4561 agatattctc tgcggtcccg tttggttggc ctccggcctg gacttgtccg gccacgcagg
     4621 gatgcttaca cttacgagtc caaagctggt gaaagggatg gcgggaggca agtacaggtc
     4681 tttcctcata cacgttaaag cagtaaacga acgggggact gaggaaatat gcaacggggg
     4741 tatgaggcca gtcgttcgcc tcccttcact caaacatcag agcaacaaag gctactccct
     4801 cgctagtttg ctcgcaaaag tggctgcggg gaaggagaaa tcttccaatg ttaagaatga
     4861 gaacaccagt ggcactcgca aatctgaaaa tcttcgaggc tgcgacctct tgcaggaagt
     4921 ttcagtgaca atcaggcgat tcaaaaaaac ttccatatct aaggagagag tacaacgctg
     4981 tgctatgttg caatttagcg aatttcatga aaagctcgtt aatacactgt gtcgaaaaac
     5041 ggatgatggg cagataaccg agcatgcgca gtctctggtc cttgacacac tctgctggct
     5101 ggcgggggta cactccaatg gcccaggtag ctccaaggaa ggtaacgaaa atctcctgtc
     5161 aaaaacccgg aaattcctga gtgatattgt gcgcgtctgc tttttcgaag ccgggcgaag
     5221 tatagcacac aaatgcgcta ggtttctcgc gctctgtatc tcaaacggta agtgcgatcc
     5281 ttgccaaccc gcgttcggtc cagtcctttt gaaggccctc ctcgataaca tgtcatttct
     5341 gccggctgcc acgactgggg gctcagtgta ctggtatttt gttcttctga actacgtcaa
     5401 ggatgaagat cttgctggct gcagtacggc ctgtgcctca cttttgactg ctgtctcccg
     5461 ccaactgcag gatcgcttga caccgatgga agcgcttctc cagacacgat atggactcta
     5521 ctcctctccg ttcgacccag tgctgttcga ccttgagatg tctgggtctt catgcaagaa
     5581 tgtatataac agcagcatag gggtccagtc tgacgaaata gatttgtccg acgtactcag
     5641 tggaaacggt aaggtttcaa gttgtacggc ggctgaaggc tcatttacta gccttaccgg
     5701 acttcttgaa gtcgagccgc tccatttcac atgtgtttca acatctgacg ggacgcggat
     5761 tgagcgagat gatgctatga gtagcttcgg tgtgacaccg gcggtgggag ggctctcctc
     5821 aggaacggta ggggaggcat caacggctct gagttccgct gcacaggtgg ctctgcagtc
     5881 tctgtcccat gccatggcca gtgcggagca gcaactccag gttctgcagg aaaagcagca
     5941 acaacttctt aagctgcaac aacagaaggc gaagttggag gcgaaactgc accaaacaac
     6001 cgcagcggca gccgcagctg cgtcagctgt cggccccgta cacaatagcg tccctagcaa
     6061 ccccgtcgct gccccgggct tctttataca cccaagcgat gtgatcccgc ctactccaaa
     6121 aacgacaccc ctgtttatga ctccaccctt gacgccgcca aatgaagctg tttctgtcgt
     6181 catcaacgca gagttggccc agcttttccc gggttcagtt attgacccgc ctgccgtcaa
     6241 tctcgcagca cacaacaaaa actcaaacaa atcccgaatg aaccctttgg ggtcaggact
     6301 tgctcttgcc atctcacacg ctagccactt ccttcagccc cctccccatc aatccatcat
     6361 aattgaacgg atgcatagtg gagctagacg cttcgttacg ttggatttcg gaaggccaat
     6421 cctcctgaca gacgttctta tacccacctg tggcgacttg gctagccttt ccattgatat
     6481 atggaccctg ggtgaagagg ttgacggtcg cagactggtt gtagcaacag acattagcac
     6541 tcactccctg attttgcatg atctcattcc gccccccgta tgtcgcttca tgaaaattac
     6601 agtcatcggc cggtatggtt caacgaatgc aagggcgaag ataccactcg gtttttacta
     6661 tggacacacg tacattcttc cttgggagtc cgagcttaag ttgatgcatg acccattgaa
     6721 aggcgaaggg gaatctgcca atcagcccga gattgatcag caccttgcca tgatggtggc
     6781 attgcaagaa gacattcagt gtcgctacaa tctggcttgt caccgcttgg aaaccttgct
     6841 ccagtccatt gatcttccac cattgaatag cgctaacaac gcgcagtact tcctgaggaa
     6901 acccgacaaa gctgtcgagg aagattctag ggtcttctct gcataccagg actgtattca
     6961 acttcagctt cagctcaacc ttgcccacaa tgctgttcaa cgcctgaagg ttgccttggg
     7021 ggcctcaaga aagatgctct ctgaaacaag taatcccgaa gacctgatac agacaagctc
     7081 cacagaacaa ctgcgaacga tcatacggta cttgctggac acgcttctct ccttgttgca
     7141 cgcaagcaat ggacattccg tgccagcagt cctgcagagc acattccatg cgcaagcttg
     7201 tgaggaactc ttcaagcatt tgtgtatttc agggacacca aagattaggc ttcacacagg
     7261 gcttttgctg gtgcaattgt gcggcgggga gaggtggtgg ggtcaatttc tttccaacgt
     7321 tttgcaggag ctttataatt ctgaacaact tttgatattt ccgcaagacc gggtgttcat
     7381 gttgctcagc tgcattggac agcgaagctt gtctaatagc ggtgtactcg aatccctgct
     7441 gaacctcttg gataatttgc tttccccgtt gcagccacag ctccccatgc accgacggac
     7501 tgaaggggtt cttgacatcc caatgatatc atgggtcgta atgttggtca gtcggttgct
     7561 tgactatgtg gcaaccgtag aggatgaagc agccgcagca aaaaaacctt tgaatggcaa
     7621 tcagtggtct ttcataaaca ataatcttca tacacaatcc ttgaaccgct ctagcaaagg
     7681 gtcaagtagc ctggatagat tgtactcaag aaaaatccgg aaacaactcg ttcatcataa
     7741 gcagcagctt aacctcctca aggcgaaaca aaaggctctg gttgagcaga tggagaaaga
     7801 aaaaattcag tcaaataagg gatctagcta taagttgctc gttgagcagg ccaaacttaa
     7861 gcaagcaacg agtaagcatt ttaaagatct cattaggctg agacggacgg ccgagtggag
     7921 tagaagtaat cttgatacag aagttaccac ggccaaggag tctccggaaa ttgaaccact
     7981 gcctttcact ttggcccatg aaaggtgcat tagcgtggtt caaaagcttg ttcttttctt
     8041 gttgtctatg gatttcacat gtcatgccga cctcttgctc ttcgtttgta aggtcttggc
     8101 acgaattgct aatgcgacca ggccaacaat acacttgtgc gaaattgtaa atgaacccca
     8161 actggaaaga ttgctgctct tgttggtcgg gacggacttc aaccgaggcg acatcagctg
     8221 gggcggcgcc tgggcccaat actcattgac ttgtatgctc caagatatac tcgcaggaga
     8281 gttgcttgct ccggtggccg ccgaagcgat ggaagaaggg accgttggtg atgatgttgg
     8341 agctacggcg ggggattccg atgatagtct gcaacaatca tcagttcagc ttctggagac
     8401 cattgacgaa ccccttaccc acgacatcac tggtgcaccc cccctgtcat cacttgagaa
     8461 agacaaagag atagatctcg agcttctcca ggaccttatg gaagtagaca tcgatccact
     8521 cgacatagac ctggagaagg acccattggc ggcaaaggta ttcaaaccca tctcatctac
     8581 ttggtacgat tattggggcg cggactacgg tacatataac tataatcctt acataggtgg
     8641 acttggaatc ccagttgcga aacctccagc gaatactgag aagaacggta gtcagactgt
     8701 atccgtgtcc gtttctcaag ccctggatgc tcgcctggaa gtgggtttgg agcagcaggc
     8761 agagttgatg ttgaaaatga tgagcactct tgaagcggac agtatattgc aggctctgac
     8821 caatacgtca cctaccttgt ctcagagccc aactgggacc gatgattcac tccttggtgg
     8881 gcttcaagct gcgaatcaaa ccagccagct gattatacag ctctcatctg tacctatgct
     8941 gaacgtttgt ttcaacaaac ttttctccat gctccaggtt caccacgtgc agcttgagtc
     9001 cttgctccaa ctgtggctta cactttccct gaatagttcc tctacaggca ataaggaaaa
     9061 tggtgcagat atctttctgt acaatgccaa tagaataccg gtgattagtc ttaatcaagc
     9121 ctctatcacc agcttcctca ccgtgctggc gtggtatccc aacacacttt tgcgcacctg
     9181 gtgcttggtt ttgcatagtc tgaccctgat gacaaatatg caattgaatt caggtagttc
     9241 cagcgctata ggaactcagg aatctacggc acacctgttg gtgtcagatc caaacctgat
     9301 ccacgtactg gttaaattcc tctctggtac ttccccgcat ggtacgaacc aacacagccc
     9361 acaagttgga cctacagcca cccaggcgat gcaggagttt ttgaccagac tccaggtaca
     9421 cctttcttca acctgccctc agatattctc agaattcctc ctcaagctta ttcatatcct
     9481 cagtaccgaa agaggggcgt tccagacggg gcaaggtcct ttggatgctc aagtcaagct
     9541 gttggagttc acccttgaac aaaacttcga ggttgtaagt gtctctacta tatctgctgt
     9601 catcgaaagc gttacgttcc ttgtacacca ttatatcaca tgctcagata aggtcatgtc
     9661 aagaagtggc tcagacagta gtgtgggcgc tcgggcttgt tttggtggtc tcttcgctaa
     9721 tctgatccga cctggagacg caaaagcagt gtgcggagag atgaccagag atcagttgat
     9781 gttcgatctt ctgaagctcg tcaacatcct tgtgcaactg cctttgagtg gcaataggga
     9841 atacagtgct agagtaagcg tcacaactaa tacgacggat tccgtttccg acgaagaaaa
     9901 agtgtccggg ggtaaagatg gaaatggttc cagcaccagc gtacaaggat ccccagccta
     9961 cgtggcggat ctcgtgctcg ccaatcaaca gataatgagt caaattctgt ccgcactcgg
    10021 cttgtgcaat tcctctgcaa tggccatgat tataggagct tctgggttgc atttgactaa
    10081 gcatgaaaac tttcatggcg gtttggatgc gatctctgtg ggagatggac ttttcacgat
    10141 tctgactact ttgagcaaga aagcgtctac ggttcacatg atgctgcagc cgatcttgac
    10201 atatatggca tgtggataca tgggccggca ggggtcactt gccacatgcc agttgtctga
    10261 gcctctgctg tggttcatac tccgagtcct cgatacttct gacgcgctta aagccttcca
    10321 tgacatggga ggagtgcagc tcatctgcaa taacatggtt acaagcacta gagcgattgt
    10381 caacacagca agatctatgg tctccaccat tatgaagttc cttgactctg ggccgaacaa
    10441 ggcagtcgat tctaccttga agacgaggat ccttgcctct gagccagata atgctgaagg
    10501 aatacacaat tttgcacctt tgggtacaat tacgagctct tctccaacgg cacagccggc
    10561 ggaagtcctg cttcaagcca ctcctcctca ccggagggcc agatccgcag cttggtcata
    10621 tatattcttg ccagaggaag catggtgcga tctcactatt catctccctg ccgcggtgct
    10681 ccttaaggaa atccatattc agcctcacct ggcttctttg gctacgtgtc catcctctgt
    10741 atcagtcgag gttagtgcgg atggagtaaa tatgctcccg ctcagtactc cggtcgtaac
    10801 ttcaggactt acctacataa agatccaact tgtaaaagct gaggtagctt ccgcggtctg
    10861 cttgcgcttg caccgccccc gcgacgcttc cacgctcggg ctttcccaga ttaaactcct
    10921 ggggcttaca gcgtttggga ccacatcttc tgctacggta aataatccgt ttttgccttc
    10981 cgaagatcaa gttagcaaaa catctatcgg atggctccgg ctcctccatc actgtctcac
    11041 gcatatctca gatctggaag gaatgatggc ttcagcggct gcccccacgg ccaatcttct
    11101 ccagacttgc gccgcacttc tcatgtcccc ctattgcgga atgcactcac ccaatattga
    11161 ggtcgtcttg gttaaaatcg gcctccagtc aactcggata ggtctcaagc ttatagacat
    11221 cttgttgcgg aattgtgcag ctagtggttc tgatcctaca gatctgaaca gtccactgtt
    11281 gtttggtcgg ttgaatggcc tctctagcga tagtacaata gacattttgt atcaacttgg
    11341 aactacccaa gatccgggaa ccaaagatcg gattcaggca ttgctcaaat gggtaagcga
    11401 tagcgcgcgc gtagcagcta tgaaaagatc tggccgaatg aattacatgt gcccgaattc
    11461 cagcactgta gaatacgggc ttctgatgcc gtcaccgagt cacctgcatt gcgttgcggc
    11521 aattctgtgg cactcctatg agttgctcgt tgaatacgac ttgccagcac tgctggatca
    11581 ggagctcttt gagcttctgt ttaattggtc catgtctctg ccgtgtaata tggttctcaa
    11641 aaaggctgtt gacagcttgc tttgctctat gtgtcacgta catcctaact acttctctct
    11701 gttgatgggt tggatgggca taaccccccc gccggttcaa tgccatcacc gcttgtccat
    11761 gacagatgac agcaaaaagc aagacttgtc ttcatctctg acagatgatt ctaaaaatgc
    11821 ccaagcaccc ttggcgctga ctgaaagcca cttggctacc ctcgcaagtt cttcacagtc
    11881 tcctgaagct attaagcagc tcctggacag cgggctgccg agcctccttg ttcggagctt
    11941 ggcttcattc tgtttttccc acataagtag tagtgaatcc attgcacaga gcattgacat
    12001 ttcacaggat aaactccggc ggcatcatgt tccccagcaa tgtaacaaaa tgccgattac
    12061 ggcggatctc gtcgccccga tattgcggtt ccttaccgag gtcggtaaca gccacatcat
    12121 gaaagactgg ctgggtggtt ccgaagtcaa tcctctctgg actgcactgc tgttcttgtt
    12181 gtgtcattct gggagtacca gcggctcaca caatctcggg gcccagcaga cgagcgctag
    12241 gtctgccagc ttgagttctg ccgctactac gggtcttaca acacaacaaa gaaccgctat
    12301 agaaaacgcg acagtggcgt tttttctcca gtgcataagt tgccacccaa ataatcaaaa
    12361 gcttatggct caagtgcttt gcgagctctt tcagaccagt ccacagaggg gcaatctccc
    12421 gacgtctggc aacataagcg gttttatcag acgactcttc cttcaactca tgctggagga
    12481 cgaaaaggtc actatgttcc tgcagagccc gtgcccgctc tataagggaa ggataaatgc
    12541 cacgagccat gtcattcagc acccgatgta cggggccggg cacaaattta gaacactcca
    12601 cctgccagta tctacgacgc tttcagatgt ccttgatagg gtctccgata ctccgagcat
    12661 caccgccaag cttatctccg agcagaaaga tgacaaagaa aaaaaaaacc acgaggaaaa
    12721 agagaaagtc aaagccgaaa atggctttca ggataactac tccgtggtag tcgcctccgg
    12781 gctcaagagt caaagcaaga gggcggtcag cgcgacccct cctcgaccac catcacgccg
    12841 cgggaggacg ataccggaca agatcggaag cacgagcggc gccgaggcag cgaataaaat
    12901 catcacggta ccagtgtttc atctgttcca taagcttctc gcaggtcagc cactgccagc
    12961 ggaaatgact ctggctcaac tgctcaccct gctttatgac cgcaagttgc ctcaggggta
    13021 cagatctata gatcttacgg ttaagctggg cagtcgggtg attacagacc cttcactgtc
    13081 caaaactgat tcctataagc gcttgcaccc tgaaaaggat cacggtgacc tccttgcttc
    13141 ctgcccagag gacgaggcct tgacacccgg cgacgaatgc atggacggaa ttttggatga
    13201 aagcctgctg gaaacctgtc cgatacaatc cccgctccag gtattcgccg ggatgggtgg
    13261 tcttgctctc atcgccgaga ggttgcccat gctttatcct gaagtgatcc agcaggtatc
    13321 agctccggtt gtaacctcaa ccacccaaga gaaacctaaa gattcagatc aatttgaatg
    13381 ggtgacaatt gagcagagcg gcgaattggt gtatgaggcc cccgaaacgg ttgctgccga
    13441 gccgccccca ataaaatccg ccgtccagac aatgtctcca ataccagcgc actctttggc
    13501 ggcatttggg ctttttctta ggctccctgg ttacgctgaa gtcctgctga aagaacgcaa
    13561 gcatgcgcaa tgccttctgc ggctcgtttt gggggtgacg gacgatggag aaggttccca
    13621 tatccttcag agccccagcg cgaacgtatt gcccacactt ccttttcatg tgctccgatc
    13681 cctcttttca actacccccc tcacaactga tgacggcgtc cttctgcgac gaatggccct
    13741 tgagatcggg gccttgcacc tcattctcgt gtgcctcagt gcgttgagcc accacagtcc
    13801 ccgggtccca aacagcagcg tgaaccagac ggaaccacag gtgagcagct cccacaaccc
    13861 cacgagtacc gaggagcagc agctctactg ggcaaaaggg accggatttg ggactggctc
    13921 aaccgcctcc ggatgggatg tggaacaagc tcttacgaag caaaggttgg aggaggaaca
    13981 tgtaacttgt ctgctccagg tgcttgcgag ctatataaac cctgtgtctt cagcggtgaa
    14041 tggcgaggca caatccagtc acgaaacgcg cggtcagaat tccaatgctc tccccagtgt
    14101 gctcctggag cttcttagcc agagttgttt gatacctgcc atgagttcat accttcgaaa
    14161 cgactctgtg ctggacatgg cgagacatgt tcccttgtat agagctcttc tggaactgct
    14221 gagagctata gccagttgcg ctgccatggt gcctctcctg ctccccctga gcaccgagaa
    14281 tggcgaggag gaggaggagc agagcgagtg ccagaccagt gttggcactc tccttgccaa
    14341 aatgaaaact tgtgtcgata cttataccaa ccgcctgcgg tctaagagag agaatgttaa
    14401 aaccggagtt aagccagacg catctgacca ggaacccgaa gggcttactt tgctcgtacc
    14461 agatatacag aaaacagctg aaattgttta cgctgcaaca acatcactcc gacaggccaa
    14521 ccaggagaag aaacttgggg agtattcaaa gaaagcggcc atgaagccta agccgctgtc
    14581 cgtcctgaaa tcccttgagg aaaagtatgt cgcagtgatg aaaaagctcc aattcgacac
    14641 ttttgaaatg gtgtctgaag acgaggacgg caagctggga ttcaaagtga actatcatta
    14701 tatgagccag gtcaaaaacg ctaacgatgc aaatagtgca gctagggctc ggcgccttgc
    14761 tcaagaagcg gttaccctca gtactagtct ccccttgtca tccagtagtt cagtcttcgt
    14821 gagatgtgat gaagagagat tggatattat gaaagttctg atcaccggcc ccgcagacac
    14881 tccttatgca aacggatgct tcgagtttga cgtttacttc ccacaggatt acccttccag
    14941 cccgcccctt gtcaatttgg aaactaccgg gggtcactcc gtacgcttca accccaacct
    15001 gtataacgac ggcaaagttt gcctctcaat tctgaataca tggcatggaa gacccgaaga
    15061 aaaatggaac ccgcaaacat catcatttct ccaggtactc gtgtccgttc aatcattgat
    15121 cctcgtggcg gaaccttact ttaacgagcc tggttacgag aggtctcgag gcacaccatc
    15181 tggtactcaa tccagcaggg agtacgatgg caatatccgc caagctacag tgaagtgggc
    15241 tatgctcgaa cagattagga atccctctcc ttgcttcaag gaggtgatac ataaacattt
    15301 ctatttgaaa agagtagaaa ttatggcgca gtgtgaagag tggatagcag acatccaaca
    15361 atattcctca gacaaacggg tcggacggac tatgtctcat cacgcagcgg ctctcaaacg
    15421 acatacggcc caactccgcg aggaactctt gaaattgccg tgtcctgagg gtttggatcc
    15481 tgacacggac gacgccccag aagtatgtag agccactaca ggtgcggaag agacccttat
    15541 gcacgatcag gtaaaaccct caagttcaaa ggaacttccg tccgactttc agctttgagc
    15601 ggccgctacc cagctttctt gtacaaagtg gttgatatcg gtaccagtcg actctagagg
    15661 atcccgggtg gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca
    15721 ctccagtgcc caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg
    15781 tgtccttcta taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga
    15841 agacaacctg tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat
    15901 cttggctcac tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg
    15961 agttgttggg attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga
    16021 gacggggttt caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc
    16081 caccttggcc tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc
    16141 ttctgatttt aaaataacta taccagcagg aggacgtcca gacacagcat aggctacctg
    16201 gccatgccca accggtggga catttgagtt gcttgcttgg cactgtcctc tcatgcgttg
    16261 ggtccactca gtagatgcct gttgaattgg gtacgcggcc agcttggctg tggaatgtgt
    16321 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    16381 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    16441 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    16501 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    16561 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
    16621 tttttggagg cctaggcttt tgcaaaaagc tcctcgagga actgaaaaac cagaaagtta
    16681 attccctata gtgagtcgta ttaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa
    16741 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    16801 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    16861 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    16921 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    16981 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    17041 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    17101 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    17161 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    17221 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    17281 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    17341 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    17401 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    17461 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    17521 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    17581 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    17641 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    17701 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    17761 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    17821 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    17881 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    17941 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    18001 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    18061 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    18121 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    18181 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    18241 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    18301 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    18361 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    18421 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    18481 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    18541 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    18601 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    18661 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    18721 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    18781 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    18841 gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc
    18901 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    18961 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    19021 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    19081 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    19141 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    19201 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    19261 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    19321 aattt
//
