LOCUS       sequence-389277-       10564 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Encoding human ErbB1 mutant..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10564)
  AUTHORS   Matsuda K, Hirayama D, Hino N, Kuno S, Sakaue-Sawano A, Miyawaki A, 
            Matsuda M, Terai K
  TITLE     Knockout of all ErbB-family genes delineates their roles in 
            proliferation, survival, and migration.
  JOURNAL   J Cell Sci. 2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199.
  PUBMED    37519219
REFERENCE   2  (bases 1 to 10564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell Sci.
            2023 Jul 31:jcs.261199. doi: 10.1242/jcs.261199."
COMMENT     Sequence Label: pPBpuro-EGFR(M721)
FEATURES             Location/Qualifiers
     source          1..10564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1392..1667
                     /label=chicken beta-actin promoter
     intron          1668..2684
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2608..2630
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2692..2711
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(2760..2831,2832..4694,4695..4763,4764..6392)
                     /codon_start=1
                     /product="precursor of human epidermal growth factor 
                     receptor"
                     /label=EGFR
                     /translation="MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFE
                     DHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENL
                     QIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQ
                     WRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGR
                     CRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNP
                     EGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGI
                     GIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVK
                     EITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISD
                     GDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWG
                     PEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGP
                     DNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEG
                     CPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTP
                     SGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIMELREATSP
                     KANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQ
                     YLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHA
                     EGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKG
                     ERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHL
                     PSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVA
                     CIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQN
                     PVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQIS
                     LDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA"
     misc_feature    6470..7019
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6614..6631)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6841..6860
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7022..7621
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7022..7041)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7518..7538
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7648..7665)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7654..7878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7925..7941)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7949..7965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7973..7979,7980..7997,7998..8003))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8018..8039
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   8273..8335
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8376..8396)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8376..8394)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8415..8431)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8439..8455
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8463..8469,8470..8487,8488..8493))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8508..8529
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8646..8663)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8817..9405)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8897..8916)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9576..10367,10368..10436))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10437..10541)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc
     1441 cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg
     1501 gggggggggg gggcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc
     1561 ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga
     1621 ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc
     1681 gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac
     1741 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1801 agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc
     1861 tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg
     1921 tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1981 cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc
     2041 ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt
     2101 gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag
     2161 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
     2221 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
     2281 ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
     2341 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
     2401 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
     2461 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
     2521 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
     2581 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2641 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2701 tggttattgt gctgtctcat cattttggca aagaattggg atccgaattc ctcgagacca
     2761 tgcgaccctc cgggacggcc ggggcagcgc tcctggcgct gctggctgcg ctctgcccgg
     2821 cgagtcgggc tctggaggaa aagaaagttt gccaaggcac gagtaacaag ctcacgcagt
     2881 tgggcacttt tgaagatcat tttctcagcc tccagaggat gttcaataac tgtgaggtgg
     2941 tccttgggaa tttggaaatt acctatgtgc agaggaatta tgatctttcc ttcttaaaga
     3001 ccatccagga ggtggctggt tatgtcctca ttgccctcaa cacagtggag cgaattcctt
     3061 tggaaaacct gcagatcatc agaggaaata tgtactacga aaattcctat gccttagcag
     3121 tcttatctaa ctatgatgca aataaaaccg gactgaagga gctgcccatg agaaatttac
     3181 aggaaatcct gcatggcgcc gtgcggttca gcaacaaccc tgccctgtgc aacgtggaga
     3241 gcatccagtg gcgggacata gtcagcagtg actttctcag caacatgtcg atggacttcc
     3301 agaaccacct gggcagctgc caaaagtgtg atccaagctg tcccaatggg agctgctggg
     3361 gtgcaggaga ggagaactgc cagaaactga ccaaaatcat ctgtgcccag cagtgctccg
     3421 ggcgctgccg tggcaagtcc cccagtgact gctgccacaa ccagtgtgct gcaggctgca
     3481 caggcccccg ggagagcgac tgcctggtct gccgcaaatt ccgagacgaa gccacgtgca
     3541 aggacacctg ccccccactc atgctctaca accccaccac gtaccagatg gatgtgaacc
     3601 ccgagggcaa atacagcttt ggtgccacct gcgtgaagaa gtgtccccgt aattatgtgg
     3661 tgacagatca cggctcgtgc gtccgagcct gtggggccga cagctatgag atggaggaag
     3721 acggcgtccg caagtgtaag aagtgcgaag ggccttgccg caaagtgtgt aacggaatag
     3781 gtattggtga atttaaagac tcactctcca taaatgctac gaatattaaa cacttcaaaa
     3841 actgcacctc catcagtggc gatctccaca tcctgccggt ggcatttagg ggtgactcct
     3901 tcacacatac tcctcctctg gatccacagg aactggatat tctgaaaacc gtaaaggaaa
     3961 tcacagggtt tttgctgatt caggcttggc ctgaaaacag gacggacctc catgcctttg
     4021 agaacctaga aatcatacgc ggcaggacca agcaacatgg tcagttttct cttgcagtcg
     4081 tcagcctgaa cataacatcc ttgggattac gctccctcaa ggagataagt gatggagatg
     4141 tgataatttc aggaaacaaa aatttgtgct atgcaaatac aataaactgg aaaaaactgt
     4201 ttgggacctc cggtcagaaa accaaaatta taagcaacag aggtgaaaac agctgcaagg
     4261 ccacaggcca ggtctgccat gccttgtgct cccccgaggg ctgctggggc ccggagccca
     4321 gggactgcgt ctcttgccgg aatgtcagcc gaggcaggga atgcgtggac aagtgcaacc
     4381 ttctggaggg tgagccaagg gagtttgtgg agaactctga gtgcatacag tgccacccag
     4441 agtgcctgcc tcaggccatg aacatcacct gcacaggacg gggaccagac aactgtatcc
     4501 agtgtgccca ctacattgac ggcccccact gcgtcaagac ctgcccggca ggagtcatgg
     4561 gagaaaacaa caccctggtc tggaagtacg cagacgccgg ccatgtgtgc cacctgtgcc
     4621 atccaaactg cacctacgga tgcactgggc caggtcttga aggctgtcca acgaatgggc
     4681 ctaagatccc gtccatcgcc actgggatgg tgggggccct cctcttgctg ctggtggtgg
     4741 ccctggggat cggcctcttc atgcgaaggc gccacatcgt tcggaagcgc acgctgcgga
     4801 ggctgctgca ggagagggag cttgtggagc ctcttacacc cagtggagaa gctcccaacc
     4861 aagctctctt gaggatcttg aaggaaactg aattcaaaaa gatcaaagtg ctgggctccg
     4921 gtgcgttcgg cacggtgtat aagggactct ggatcccaga aggtgagaaa gttaaaattc
     4981 ccgtcgcgat catggaatta agagaagcaa catctccgaa agccaacaag gaaatcctcg
     5041 atgaagccta cgtgatggcc agcgtggaca acccccacgt gtgccgcctg ctgggcatct
     5101 gcctcacctc caccgtgcag ctcatcacgc agctcatgcc cttcggctgc ctcctggact
     5161 atgtccggga acacaaagac aatattggct cccagtacct gctcaactgg tgtgtgcaga
     5221 tcgcaaaggg catgaactac ttggaggacc gtcgcttggt gcaccgcgac ctggcagcca
     5281 ggaacgtact ggtgaaaaca ccgcagcatg tcaagatcac agattttggg ctggccaaac
     5341 tgctgggtgc ggaagagaaa gaataccatg cagaaggagg caaagtgcct atcaagtgga
     5401 tggcattgga atcaatttta cacagaatct atacccacca gagtgatgtc tggagctacg
     5461 gggtgaccgt ttgggagttg atgacctttg gatccaagcc atatgacgga atccctgcca
     5521 gcgagatctc ctccatcctg gagaaaggag aacgcctccc tcagccaccc atatgtacca
     5581 tcgatgtcta catgatcatg gtcaagtgct ggatgataga cgcagatagt cgcccaaagt
     5641 tccgtgagtt gatcatcgaa ttctccaaaa tggcccgaga cccccagcgc taccttgtca
     5701 ttcaggggga tgaaagaatg catttgccaa gtcctacaga ttccaacttc taccgtgccc
     5761 tgatggatga agaagacatg gacgacgtgg tggatgccga cgagtacctc atcccacagc
     5821 agggcttctt cagcagcccc tccacgtcac ggactcccct cctgagctct ctgagtgcaa
     5881 ccagcaacaa ttccaccgtg gcttgcattg atagaaatgg gctgcaaagc tgtcccatca
     5941 aggaagacag cttcttgcag cgatacagct cagaccccac aggcgccttg actgaggaca
     6001 gcatagacga caccttcctc ccagtgcctg aatacataaa ccagtccgtt cccaaaaggc
     6061 ccgctggctc tgtgcagaat cctgtctatc acaatcagcc tctgaacccc gcgcccagca
     6121 gagacccaca ctaccaggac ccccacagca ctgcagtggg caaccccgag tatctcaaca
     6181 ctgtccagcc cacctgtgtc aacagcacat tcgacagccc tgcccactgg gcccagaaag
     6241 gcagccacca aattagcctg gacaaccctg actaccagca ggacttcttt cccaaggaag
     6301 ccaagccaaa tggcatcttt aagggctcca cagctgaaaa tgcagaatac ctaagggtcg
     6361 cgccacaaag cagtgaattt attggagcat gagcggccgc tctagagtca actaacttaa
     6421 gctagcaacg gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc
     6481 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg
     6541 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct
     6601 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca
     6661 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg
     6721 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct
     6781 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa
     6841 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt
     6901 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa
     6961 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgataatac
     7021 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     7081 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7141 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7201 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7261 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7321 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7381 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7441 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7501 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7561 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7621 atcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7681 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7741 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7801 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7861 atgcggtggg ctctatggct tctgaggcgg aaagaacctg cagcccaagc ttggcgtaat
     7921 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7981 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     8041 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag cggatcttaa
     8101 aagttttgtt actttataga agaaattttg agtttttgtt tttttttaat aaataaataa
     8161 acataaataa attgtttgtt gaatttatta ttagtatgta agtgtaaata taataaaact
     8221 taatatctat tcaaattaat aaataaacct cgatatacag accgataaaa cacatgcgtc
     8281 aattttacgc atgattatct ttaacgtacg tcacaatatg attatctttc tagggttaat
     8341 ctagtatacg cgtttaatta atccagcttt tgttcccttt agtgagggtt aattgcgcgc
     8401 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8461 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8521 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8581 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8641 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8701 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8761 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8821 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8881 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8941 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9001 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9061 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9121 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9181 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9241 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9301 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     9361 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9421 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9481 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9541 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9601 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9661 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9721 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9781 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9841 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9901 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9961 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10021 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10081 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10141 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10201 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10261 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10321 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10381 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10441 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10501 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10561 ccac
//
